| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,230,217 | A→G | 36.0% | intergenic (+322/‑104) | ygjK → / → fadH | predicted glycosyl hydrolase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,230,217 | 0 | A | G | 36.0% | 43.0 / 50.2 | 50 | intergenic (+322/‑104) | ygjK/fadH | predicted glycosyl hydrolase/2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked |
| Reads supporting (aligned to +/- strand): ref base A (20/12); new base G (13/5); total (33/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
CAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAG > W3110S.gb/3230147‑3230274 | cAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGaa > 1:358672/1‑71 (MQ=255) aTTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTc > 1:2156360/1‑71 (MQ=255) ccGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCa > 1:3679501/1‑71 (MQ=255) ccGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCa > 1:3600122/1‑71 (MQ=255) ccGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCa > 1:2922643/1‑71 (MQ=255) aaCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAAt > 1:1285792/1‑63 (MQ=255) aaCCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAAt > 1:1510738/1‑63 (MQ=255) ccTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACaa < 1:1343663/71‑1 (MQ=255) gATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACAAAccc > 1:2679363/1‑71 (MQ=255) gATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACAAAccc > 1:2311686/1‑71 (MQ=255) gATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAAccc > 1:3769814/1‑71 (MQ=255) tAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACaaa > 1:1150334/1‑66 (MQ=255) cgcgACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATc > 1:126555/1‑51 (MQ=255) cgcgACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATc > 1:2025715/1‑51 (MQ=255) cgcgACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATc > 1:1543312/1‑51 (MQ=255) cgACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTccc > 1:2455361/1‑71 (MQ=255) cgACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTccc > 1:2541469/1‑71 (MQ=255) gACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTcccc < 1:3415286/71‑1 (MQ=255) gACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTcccc < 1:1649220/71‑1 (MQ=255) aCAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTcccc < 1:361705/70‑1 (MQ=255) aaGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACAAACCCCGCACt > 1:1504044/1‑64 (MQ=255) tCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCcccttttccc < 1:1707603/70‑1 (MQ=255) gCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACAAACCCCGCACTCcccttttcccttt < 1:75907/71‑1 (MQ=255) gCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACaaa > 1:177241/1‑48 (MQ=255) gCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACaaa > 1:3703571/1‑48 (MQ=255) cGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTcc < 1:2149599/71‑1 (MQ=255) cGGCGTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTcc < 1:2670407/71‑1 (MQ=255) cGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTtctc > 1:865034/1‑70 (MQ=255) cGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGc < 1:3266604/71‑1 (MQ=255) gTTATCACCGGGCGTATTCTTTTTGAGTCCCATCACAAACCCCGCACTCcccttttccctt > 1:3669544/1‑61 (MQ=255) gTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCg < 1:3660511/44‑1 (MQ=255) aTCACCGGGCGTATTCTTTTTGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACg > 1:2003816/1‑71 (MQ=255) aTCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGAc > 1:3100477/1‑70 (MQ=255) aCCGGGCGTATTCTTTTTGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCt < 1:198460/71‑1 (MQ=255) tATTCTTTTTGAGTCCCATCACAAACCCCGCACTcc < 1:842733/36‑1 (MQ=255) tATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGc < 1:2709083/56‑1 (MQ=255) tATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGc < 1:3670858/56‑1 (MQ=255) tATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTa < 1:856140/64‑1 (MQ=255) ttCTTTTTGAGTCCCATCACAAACCCCGCACTcccc > 1:3410139/1‑36 (MQ=255) ttCTTTTTGAGTCCCATCACAAACCCCGCACTcccc > 1:3706909/1‑36 (MQ=255) ttCTTTTTGAGTCCCATCACAAACCCCGCACTcccc > 1:695313/1‑36 (MQ=255) ttCTTTTTGAGTCCCATCACAAACCCCGCACTccc > 1:2392806/1‑35 (MQ=255) ttCTTTTTGAATCCCATCACAAACCCCGCCCTCcccttttcccttt > 1:3811347/1‑46 (MQ=255) ttCTTTTTGAATCCCATCACAAACCCCGCACTCcccttttcccttt > 1:2839759/1‑46 (MQ=255) ttCTTTTTGAATCCCATCACAAACCCCGCACTCcccttttcccttt > 1:2053496/1‑46 (MQ=255) ttCTTTTTGAATCCCATCACAAACCCCGCACTCcccttttccc < 1:1735840/43‑1 (MQ=255) cTTTTTGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCgg > 1:2956347/1‑51 (MQ=255) cTTTTTGAGTCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCg > 1:1578786/1‑50 (MQ=255) cTTTTTGAATCCCATCACAAACCCCGCACTCcccttttccctt > 1:1441420/1‑43 (MQ=255) cTTTTTGAATCCCATCACAAACCCCGCACTCcccttttccctt > 1:1464299/1‑43 (MQ=255) tttttGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAg < 1:3479397/65‑1 (MQ=255) | CAATTCCGTAACCTGGATAAAACGCGACAAGCGTCGCATCCGGCGTTATCACCGGGCGTATTCTTTTTGAATCCCATCACAAACCCCGCACTCCCCTTTTCCCTTTTCTCCGGCGACGGCTAAATTAG > W3110S.gb/3230147‑3230274 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |