| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,232,470 | A→G | 16.0% | L111L (TTG→CTG) | ygjM ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,232,470 | 0 | A | G | 16.0% | 118.7 / 16.3 | 50 | L111L (TTG→CTG) | ygjM | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (29/13); new base G (4/4); total (33/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATA > W3110S.gb/3232400‑3232540 | gCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCaa < 1:3628299/71‑1 (MQ=255)gCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCaa < 1:200728/71‑1 (MQ=255)gCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCaa < 1:2074074/71‑1 (MQ=255) ggAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCaa < 1:3163305/68‑1 (MQ=255) ggAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCaa < 1:289903/68‑1 (MQ=255) ggAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCaa < 1:270749/68‑1 (MQ=255) ggAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCaa < 1:2215239/68‑1 (MQ=255) agaAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAcgc > 1:2621398/1‑71 (MQ=255) aaaTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt < 1:2797410/71‑1 (MQ=255) aaaTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGt < 1:2379356/71‑1 (MQ=255) aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa > 1:3413388/1‑71 (MQ=255) aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa > 1:2832249/1‑71 (MQ=255) aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa > 1:2729260/1‑71 (MQ=255) aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGa > 1:988304/1‑71 (MQ=255) aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGa > 1:721868/1‑71 (MQ=255) tGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGac < 1:2369153/71‑1 (MQ=255) ccGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGa > 1:845499/1‑68 (MQ=255) ccGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGa > 1:1355883/1‑68 (MQ=255) gAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCa < 1:259109/71‑1 (MQ=255) gcgTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGa < 1:2585470/70‑1 (MQ=255) gCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACt > 1:1264670/1‑71 (MQ=255) ttttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCaa < 1:2449962/42‑1 (MQ=255) ttttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCaa < 1:1266858/42‑1 (MQ=255) ttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAAtt > 1:2348728/1‑71 (MQ=255) tgtTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCgg > 1:573687/1‑68 (MQ=255) ttCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCgg > 1:2385823/1‑66 (MQ=255) ttCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCa < 1:3361629/68‑1 (MQ=255) ccAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGa > 1:2965301/1‑46 (MQ=255) ccAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGa > 1:1039700/1‑46 (MQ=255) ccAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGa > 1:1218078/1‑46 (MQ=255) ccAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGa > 1:555117/1‑46 (MQ=255) ccAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCgg > 1:2269366/1‑64 (MQ=255) ccAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCgg > 1:1398808/1‑64 (MQ=255) ccAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCgg > 1:1578252/1‑64 (MQ=255) ccAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCg > 1:2388467/1‑71 (MQ=255) aGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGa < 1:2287024/71‑1 (MQ=255) gCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCa < 1:3768293/53‑1 (MQ=255) gCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGaa < 1:297347/71‑1 (MQ=255) ttAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGc > 1:183346/1‑59 (MQ=255) ttAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGc > 1:809088/1‑59 (MQ=255) ttAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGc > 1:3339800/1‑59 (MQ=255) tAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAggg > 1:778534/1‑71 (MQ=255) aaTTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGt > 1:3960535/1‑71 (MQ=255) aTTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGt < 1:3613891/70‑1 (MQ=255) tttCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTa < 1:764164/71‑1 (MQ=255) tCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTaaa > 1:2595217/1‑71 (MQ=255) ttCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCa < 1:2557027/69‑1 (MQ=255) ttCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCa < 1:3756712/69‑1 (MQ=255) cccGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGAt > 1:2927751/1‑50 (MQ=255) cccGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCat < 1:372140/68‑1 (MQ=255) cccGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCat < 1:2223104/68‑1 (MQ=255) cccGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATAtt > 1:649512/1‑71 (MQ=255) cccGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATAtt > 1:2110289/1‑71 (MQ=255) cccGCTCAAAACGCGTGACACCATAGATTTACTGCCAACTTCCGGCAGATCGGAAAGGGTTAAACCat < 1:1964875/68‑1 (MQ=255) cTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATc < 1:2753943/71‑1 (MQ=255) cAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTg > 1:1219002/1‑66 (MQ=255) cAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGa > 1:3496540/1‑67 (MQ=255) cAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGa > 1:3491800/1‑67 (MQ=255) cAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGa > 1:1236356/1‑67 (MQ=255) aaaCGCGTGACACCATAGATTTACTTCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATa < 1:2649259/71‑1 (MQ=255) | GCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATA > W3110S.gb/3232400‑3232540 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |