| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,272,348 | C→T | 24.4% | G89S (GGT→AGT) | garR ← | tartronate semialdehyde reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,272,348 | 0 | C | T | 24.4% | 151.7 / 49.9 | 82 | G89S (GGT→AGT) | garR | tartronate semialdehyde reductase |
| Reads supporting (aligned to +/- strand): ref base C (28/33); new base T (11/9); total (40/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
TCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGGCAG > W3110S.gb/3272282‑3272417 | tCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGc > 1:3562303/1‑71 (MQ=255)tCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGc > 1:3357519/1‑71 (MQ=255) tCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCgcgc > 1:1247336/1‑70 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCaat < 1:432846/71‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCaat < 1:2088461/71‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCaat < 1:376002/71‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaat < 1:2890193/71‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaat < 1:3303732/71‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaa < 1:1964692/70‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCa < 1:3482035/69‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCa < 1:3636314/69‑1 (MQ=255) ttCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACGTGGCTTCGCGCCTTCaataa > 1:1199109/1‑71 (MQ=255) ttCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaataa > 1:763866/1‑71 (MQ=255) ttCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaataa > 1:235336/1‑71 (MQ=255) ttCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaataa > 1:376716/1‑71 (MQ=255) ttCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaataa > 1:2930214/1‑71 (MQ=255) ttCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaataa > 1:2073673/1‑71 (MQ=255) ttCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaataa > 1:3893657/1‑71 (MQ=255) aCGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGc < 1:561087/71‑1 (MQ=255) ccAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATtctc < 1:1913225/70‑1 (MQ=255) gCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCAccc < 1:474016/71‑1 (MQ=255) gCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCAccc < 1:3827197/71‑1 (MQ=255) gCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCAccc < 1:1122007/71‑1 (MQ=255) tGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAgcg > 1:1002290/1‑71 (MQ=255) tGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAgcg > 1:2443866/1‑71 (MQ=255) cGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaataa > 1:1118256/1‑51 (MQ=255) cGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAgcgc < 1:577912/70‑1 (MQ=255) aTAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATTCCATTCTCACCCAGCGCCAc < 1:720608/71‑1 (MQ=255) aaCTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACctct < 1:73371/71‑1 (MQ=255) aCTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCtt > 1:3982787/1‑71 (MQ=255) aCTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCtt > 1:2303348/1‑71 (MQ=255) cTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGc < 1:2410789/46‑1 (MQ=255) cTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGc < 1:168038/46‑1 (MQ=255) cTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGc < 1:1439091/46‑1 (MQ=255) cTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGc < 1:3104704/46‑1 (MQ=255) cATATCGATCAATACCGTACTTGGCTTCCCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTca > 1:3813997/1‑71 (MQ=255) atatCGATCAATACCGTACTTGGCTTCGCGCCTTCaataa > 1:3631748/1‑40 (MQ=255) atatCGATCAATACCGTACTTGGCTTCGCGCCTTCaataa > 1:661474/1‑40 (MQ=255) atatCGATCAATACCGTACTTGGCTTCGCGCCTTCaataa > 1:3021207/1‑40 (MQ=255) atatCGATCAATACCGTACTTGGCTTCGCGCCTTCaataa > 1:1102235/1‑40 (MQ=255) tatCGATCAATACCGTACTTGGCTTCGCGCCTTCaat < 1:733382/37‑1 (MQ=255) tCGATCAATACCGTACTTGGCTTCGCGCCTTCaataa > 1:1383160/1‑37 (MQ=255) tCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCAGCTCTTTcaca > 1:805876/1‑69 (MQ=255) tCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt > 1:1218200/1‑70 (MQ=255) tCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATg < 1:3264429/71‑1 (MQ=255) tCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATg < 1:1172967/71‑1 (MQ=255) tCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATg < 1:3941760/71‑1 (MQ=255) tCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATg < 1:3757067/71‑1 (MQ=255) tCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATg < 1:3575705/71‑1 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCa > 1:3684322/1‑42 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCa > 1:3807268/1‑42 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCa > 1:1315065/1‑42 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCa > 1:2655861/1‑42 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACctct < 1:3917165/63‑1 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATg < 1:2599987/71‑1 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATg < 1:447664/71‑1 (MQ=255) gATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAg > 1:3349657/1‑71 (MQ=255) gATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATtct > 1:3088252/1‑44 (MQ=255) tACCGTACCTGGCTTCGCGCCTTCAATAATGCCAtt < 1:2758919/36‑1 (MQ=255) tACCGTACCTGGCTTCGCGCCTTCAATAATGCCAtt < 1:1439841/36‑1 (MQ=255) tACCGTACCTGGCTTCGCGCCTTCAATAATGCCAtt < 1:2977367/36‑1 (MQ=255) tACCGTACCTGGCTTCGCGCCTTCAATAATGCCATtct < 1:403137/38‑1 (MQ=255) tACCGTACCTGGCTTCGCGCCTTCAATAATGCCATtct < 1:2013730/38‑1 (MQ=255) tACCGTACCTGGCTTCGCGCCTTCAATAATGCCATtct < 1:404904/38‑1 (MQ=255) tACCGTACCTGGCTTCGCGCCTTCAATAATGCCATtct < 1:2443311/38‑1 (MQ=255) tACCGTACCTGGCTTCGCGCCTTCAATAATGCCATtct < 1:1519936/38‑1 (MQ=255) tACCGTACCTGGCTTCGCGCCTTCAATAATGCCATtct < 1:731368/38‑1 (MQ=255) tACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGcc < 1:259165/49‑1 (MQ=255) tACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTcac < 1:320606/60‑1 (MQ=255) tACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAg < 1:1220429/70‑1 (MQ=255) aCCGTACCTGGCTTCGCGCCTTCAATAATGCCAtt < 1:629590/35‑1 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTcaca > 1:1919003/1‑59 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTcaca > 1:1850551/1‑59 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt > 1:1533060/1‑60 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt > 1:2477590/1‑60 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt > 1:705086/1‑60 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt > 1:3725344/1‑60 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt > 1:3609427/1‑60 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt > 1:3318776/1‑60 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGttt > 1:204868/1‑71 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGttt > 1:2075679/1‑71 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGttt > 1:3666818/1‑71 (MQ=255) ccTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGgcag > 1:586009/1‑71 (MQ=255) cTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt < 1:1538828/54‑1 (MQ=255) cTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGgcag < 1:3653552/70‑1 (MQ=255) | TCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGGCAG > W3110S.gb/3272282‑3272417 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |