| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,321,282 | C→T | 6.3% | V63I (GTT→ATT) | yhbX ← | predicted hydrolase, inner membrane |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,321,282 | 0 | C | T | 6.3% | 302.4 / 7.5 | 95 | V63I (GTT→ATT) | yhbX | predicted hydrolase, inner membrane |
| Reads supporting (aligned to +/- strand): ref base C (40/49); new base T (3/3); total (43/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
CCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAG > W3110S.gb/3321212‑3321345 | ccAAAGGTACACCATGAATAGAGGCTAATACTCATG‑‑TGCCGCAGTCAGTAACACAAATGGTGCGGcaaca < 1:1930101/70‑1 (MQ=255) cAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGcaaca < 1:767613/71‑1 (MQ=255) aaaGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGcaac > 1:342097/1‑69 (MQ=255) aaaGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGcaac > 1:3822199/1‑69 (MQ=255) aaaGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGcaac > 1:1306128/1‑69 (MQ=255) aaGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAg < 1:1317981/71‑1 (MQ=255) acacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAATAAGCAGGc > 1:250166/1‑71 (MQ=255) acacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAATAAGCAGGc > 1:368489/1‑71 (MQ=255) acacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAATAAGCAGGc > 1:1183174/1‑71 (MQ=255) cacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCg < 1:487261/71‑1 (MQ=255) cacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCg < 1:335446/71‑1 (MQ=255) cacCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCg < 1:1747362/71‑1 (MQ=255) aTGAATAGAGGCTAATACTCATGTCTTCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCa > 1:3184386/1‑63 (MQ=255) aTGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCa > 1:3532870/1‑63 (MQ=255) tGAATAGAGGCTAATACTCATGTCTGCCGGAGTCAGTAACACAATTGGTGCGGCAACAAg < 1:1498740/60‑1 (MQ=255) aaTAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCgg < 1:703650/70‑1 (MQ=255) aaTAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGa < 1:3852207/71‑1 (MQ=255) aaTAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGa < 1:2981830/71‑1 (MQ=255) gagGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAATAAGCAGGCGAAGCGGAAggg < 1:193457/71‑1 (MQ=255) gagGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAATAAGCAGGCGAAGCGGAAggg < 1:945324/71‑1 (MQ=255) agGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAATAAGCAGGCGAAGCGGAAggg < 1:1466473/70‑1 (MQ=255) tAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:1950616/1‑70 (MQ=255) aTACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGata > 1:178746/1‑70 (MQ=255) aTACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGata > 1:173793/1‑70 (MQ=255) aTACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGata < 1:2377017/70‑1 (MQ=255) aTACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGata > 1:1854921/1‑70 (MQ=255) tACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGaatg < 1:1980973/62‑3 (MQ=255) tACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGa < 1:764131/59‑1 (MQ=255) tACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAgg < 1:780368/62‑1 (MQ=255) tACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAgg < 1:1478153/62‑1 (MQ=255) tACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGAtata < 1:2133702/71‑1 (MQ=255) tACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGAtata < 1:2762097/71‑1 (MQ=255) tACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGAtata < 1:1533364/71‑1 (MQ=255) aCTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGata > 1:2732712/1‑68 (MQ=255) aCTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATAc > 1:1883375/1‑71 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGaa > 1:2044852/1‑58 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACg > 1:2097840/1‑71 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACg > 1:2180071/1‑71 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACg > 1:2945211/1‑71 (MQ=255) cTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACg > 1:152592/1‑71 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGtt > 1:2909748/1‑71 (MQ=255) cATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGtt > 1:3870106/1‑71 (MQ=255) aTGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt < 1:1037043/71‑1 (MQ=255) aTGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt < 1:2832837/71‑1 (MQ=255) aTGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt < 1:2828008/71‑1 (MQ=255) aTGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:534947/1‑71 (MQ=255) aTGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt < 1:495751/71‑1 (MQ=255) aTGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGttt > 1:4017749/1‑71 (MQ=255) tCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTa > 1:3111220/1‑70 (MQ=255) tGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACaa > 1:3026314/1‑71 (MQ=255) ccGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAAc < 1:3486470/70‑1 (MQ=255) ccGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAAc < 1:3751822/70‑1 (MQ=255) ccGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAAc < 1:2017010/70‑1 (MQ=255) gCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAg > 1:3608264/1‑71 (MQ=255) gCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAg > 1:393071/1‑71 (MQ=255) gCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGCATGATATACGTTTTACAACCAg < 1:1892217/71‑1 (MQ=255) agtcagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAg < 1:3380486/69‑1 (MQ=255) tcagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTAc < 1:4002561/61‑1 (MQ=255) tcagtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACa < 1:1942057/62‑1 (MQ=255) agtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGt > 1:3359739/1‑71 (MQ=255) agtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGt > 1:3943068/1‑71 (MQ=255) gtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga < 1:2977359/46‑1 (MQ=255) gtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga < 1:3754161/46‑1 (MQ=255) gtAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga < 1:3375347/46‑1 (MQ=255) tAACACAAATTGTGCGGCAACAAGCAGGCGAAGCGGAAggg < 1:3596407/41‑1 (MQ=255) tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCTGAAggg < 1:953595/41‑1 (MQ=255) tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgat < 1:1530651/43‑1 (MQ=255) tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgat < 1:3930863/43‑1 (MQ=255) tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAgg < 1:1886196/64‑1 (MQ=255) tAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAgg < 1:2960022/64‑1 (MQ=255) aaCACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGt > 1:2599742/1‑68 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:3509340/1‑43 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:1289679/1‑43 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:328337/1‑43 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCa > 1:3849180/1‑60 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAg > 1:921773/1‑61 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAg > 1:2148382/1‑61 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGAt > 1:2229725/1‑64 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAg < 1:3383582/70‑1 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAg < 1:3572214/70‑1 (MQ=255) acacAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAg < 1:3567626/70‑1 (MQ=255) acaAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAAc > 1:2762724/1‑71 (MQ=255) acaAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAAc > 1:2602591/1‑71 (MQ=255) caAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:656147/1‑40 (MQ=255) caAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGgatga > 1:3507469/1‑40 (MQ=255) caAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAAcc < 1:1945393/56‑1 (MQ=255) caAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAAc > 1:3168632/1‑70 (MQ=255) aTGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACgaag < 1:4004764/71‑1 (MQ=255) aTGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACgaag < 1:2851904/71‑1 (MQ=255) aTGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACgaag < 1:613754/71‑1 (MQ=255) aTGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACgaag < 1:877763/71‑1 (MQ=255) gTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACgaagaa < 1:1285587/70‑1 (MQ=255) gTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACgaagaa < 1:2323868/70‑1 (MQ=255) gTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACgaagaa < 1:1349233/70‑1 (MQ=255) gTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACgaagaa < 1:218426/70‑1 (MQ=255) gTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACgaagaa < 1:3786708/70‑1 (MQ=255) gCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAg > 1:3407154/1‑71 (MQ=255) cGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAAcc > 1:3350413/1‑47 (MQ=255) cGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAg > 1:1100446/1‑49 (MQ=255) acaaGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAAc < 1:118797/41‑1 (MQ=255) | CCAAAGGTACACCATGAATAGAGGCTAATACTCATGTCTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGAAGCGGAAGGGATGATATACGTTTTACAACCAGGATGGTCAGAACGAAGAACAG > W3110S.gb/3321212‑3321345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |