| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,331,819 | A→G | 17.1% | F258L (TTT→CTT) | yhbE ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,331,819 | 0 | A | G | 17.1% | 75.1 / 11.1 | 35 | F258L (TTT→CTT) | yhbE | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (17/12); new base G (2/4); total (19/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-01 | |||||||||||
TTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGC > W3110S.gb/3331752‑3331888 | tttAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAGCAg > 1:1104592/1‑71 (MQ=255) ttAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGt > 1:465209/1‑71 (MQ=255) tAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTaaa > 1:2626522/1‑66 (MQ=255) tAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTaaa > 1:3666767/1‑66 (MQ=255) tAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTg > 1:3259169/1‑71 (MQ=255) aaCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTg < 1:59296/70‑1 (MQ=255) aCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTggg < 1:3850579/71‑1 (MQ=255) aCATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTgg < 1:1401576/70‑1 (MQ=255) tCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGt > 1:3244769/1‑70 (MQ=255) tCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGt > 1:2010292/1‑70 (MQ=255) tCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGt > 1:2236098/1‑70 (MQ=255) ggTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTaaa > 1:2221952/1‑59 (MQ=255) ggTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAGCAGTGGGGTGAg < 1:555042/71‑1 (MQ=255) ggTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAGCAGTGGGGTGAg < 1:1050321/71‑1 (MQ=255) gCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAGCAGTGGGGTGAGCGtgat > 1:1446546/1‑71 (MQ=255) gCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGtgat > 1:3757518/1‑71 (MQ=255) gaaATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGt > 1:3880378/1‑52 (MQ=255) gaaATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTggg > 1:3490137/1‑55 (MQ=255) gaaATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACACCGTAAACAGTggg > 1:3280445/1‑55 (MQ=255) cGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTggg > 1:2194660/1‑50 (MQ=255) agTGATAAATGATCTGTAAAAAACAGCGTAAGCAGTGGGGTGAg < 1:1084743/44‑1 (MQ=37) gTGATAAAAGATCTGAAAAAAACAGCGTAAGCAGTggg < 1:1113142/38‑1 (MQ=255) gTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTgg < 1:2525784/37‑1 (MQ=255) gTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTgg < 1:1722539/37‑1 (MQ=255) gATAAAAGATCTGAAAAAGACAGCGTAAACAGTGGGGTGa > 1:1862961/1‑40 (MQ=255) tAAAAGATCTGAAAAAAACAGCATAAACAGTGGGGTGAGCGtg < 1:380596/43‑1 (MQ=255) aaaaGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGctgcctgc > 1:434549/1‑69 (MQ=255) aaaGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGtgatg < 1:1179275/44‑1 (MQ=255) aaaGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGtgat > 1:3107193/1‑43 (MQ=255) gATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCa > 1:1214292/1‑67 (MQ=255) gATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGc > 1:3446368/1‑69 (MQ=255) aaaaaaaCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCa < 1:1063351/69‑1 (MQ=255) aaaaaaaCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCAt < 1:782002/70‑1 (MQ=255) aaaaaaaCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATc < 1:479749/71‑1 (MQ=255) aaaaaaaCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATc < 1:2658487/71‑1 (MQ=255) aCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCt > 1:2993654/1‑37 (MQ=255) aCAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCt > 1:2746445/1‑37 (MQ=255) gCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGcc < 1:3463030/71‑1 (MQ=255) aaCAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGgc > 1:3334035/1‑71 (MQ=255) | TTTAACATCGGTCTGGCGAAGAAATCGGGCCAGGCCAGTGATAAAAGATCTGAAAAAAACAGCGTAAACAGTGGGGTGAGCGTGATGATCGCGCTCACCTGCGCTGCCTGCCAGCGAGCCATCGCTTCCGCCAGGGC > W3110S.gb/3331752‑3331888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |