| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,343,341 | T→C | 23.1% | I36T (ATC→ACC) | yhbN → | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,343,341 | 0 | T | C | 23.1% | 122.5 / 38.0 | 65 | I36T (ATC→ACC) | yhbN | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (29/21); new base C (10/5); total (39/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTC > W3110S.gb/3343279‑3343408 | cAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTg < 1:1310155/71‑1 (MQ=255)cAGCTCACTTCTGGCC‑CCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGa < 1:1415428/71‑1 (MQ=255) gCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTTATCAGCCGACCCACATTg < 1:4041705/69‑1 (MQ=255) gCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTg < 1:2875032/69‑1 (MQ=255) gCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTg < 1:1798108/69‑1 (MQ=255) cACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCg > 1:176838/1‑71 (MQ=255) aCTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATc < 1:2684616/69‑1 (MQ=255) cTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGAc > 1:1620545/1‑69 (MQ=255) gccgccAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCa < 1:751402/71‑1 (MQ=255) gccAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAAtc > 1:3968628/1‑71 (MQ=255) gccAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAAtc > 1:3750150/1‑71 (MQ=255) aTTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGa > 1:2733129/1‑71 (MQ=255) ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCgg > 1:1014326/1‑52 (MQ=255) ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGatat > 1:1494244/1‑71 (MQ=255) ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGatat > 1:1865090/1‑71 (MQ=255) cGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGatat < 1:2205044/70‑1 (MQ=255) gCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGAcc > 1:107023/1‑52 (MQ=255) tttGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCa < 1:1218132/53‑1 (MQ=255) tttGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCa < 1:2728435/53‑1 (MQ=255) tttGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGc < 1:470765/52‑1 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAAt > 1:3239679/1‑53 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAAt > 1:207337/1‑53 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGata > 1:3252512/1‑62 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGata > 1:2947714/1‑62 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAg > 1:2765894/1‑68 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc < 1:600876/70‑1 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGC‑ATCTCTTGATATGCAAGGCa < 1:2007395/70‑1 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGCCCAGCAATCTCTTGATATGCAAGGc > 1:1739662/1‑70 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc > 1:3469335/1‑70 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc > 1:893856/1‑70 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc > 1:3536347/1‑70 (MQ=255) ccGTAACCGGAGACACTGATCAGCCGATCCACATTGAATc > 1:3334241/1‑40 (MQ=255) ccGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAAc > 1:1048332/1‑71 (MQ=255) tAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGTAAccac < 1:2138188/71‑4 (MQ=255) tAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTg < 1:3891818/71‑1 (MQ=255) aCCGGAGACACTGATCAGCCGATCCACATTGAATCGTACCAGCAATCTCTTGATATg > 1:245895/1‑57 (MQ=255) aCCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCaa > 1:4004620/1‑45 (MQ=255) aCCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATg > 1:2175012/1‑57 (MQ=255) aCCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGt > 1:3112137/1‑71 (MQ=255) ggAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc < 1:2962873/71‑1 (MQ=255) acacTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCtt < 1:2099908/44‑1 (MQ=255) cacTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTa > 1:3362159/1‑71 (MQ=255) cacTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTa > 1:3384317/1‑71 (MQ=255) cacTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTa > 1:3388900/1‑71 (MQ=255) cacTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTa > 1:4052099/1‑71 (MQ=255) acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc > 1:2615952/1‑65 (MQ=255) acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc > 1:2607043/1‑65 (MQ=255) acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc > 1:1031787/1‑65 (MQ=255) acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc > 1:1231385/1‑65 (MQ=255) acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAc > 1:1498025/1‑71 (MQ=255) acTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAc > 1:2056210/1‑71 (MQ=255) cTGATCAGCCGATCCACATTGAATCGGACCAGCAAtc < 1:210819/37‑1 (MQ=255) cTGATCAGCCGATCCACATTGAATCGGACCAGCAAtc < 1:3720426/37‑1 (MQ=255) cTGATCAGCCGATCCACATTGAATCGGACCAGCAAtc < 1:2488220/37‑1 (MQ=255) cTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAcc > 1:412456/1‑71 (MQ=255) cTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAcc > 1:1028040/1‑71 (MQ=255) tGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAcct > 1:847952/1‑70 (MQ=255) gATCAGCCGATCCACATTGAATCGGACCAGCAAtctc > 1:3405822/1‑37 (MQ=255) aTCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt < 1:3796675/71‑1 (MQ=255) aTCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt < 1:1906312/71‑1 (MQ=255) tCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTa < 1:2329325/71‑1 (MQ=255) cAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTgg > 1:921636/1‑55 (MQ=255) cGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTTGTTACCTTTACCGGTAATGTc < 1:3597418/71‑1 (MQ=255) aTCCACATTGAATCGGACCAGCAATCTCTTGATATGCTAGGCa < 1:3596639/43‑1 (MQ=255) aTCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCa < 1:2753523/43‑1 (MQ=255) | CAGCTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTC > W3110S.gb/3343279‑3343408 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |