| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 237,726 | A→G | 15.1% | N131S (AAC→AGC) | yafT → | predicted aminopeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 237,726 | 0 | A | G | 15.1% | 130.9 / 16.1 | 53 | N131S (AAC→AGC) | yafT | predicted aminopeptidase |
| Reads supporting (aligned to +/- strand): ref base A (15/30); new base G (3/5); total (18/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.44e-01 | |||||||||||
TCCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGA > W3110S.gb/237656‑237793 | tCCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGaa > 1:2254214/1‑71 (MQ=255)tCCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGaa > 1:2823377/1‑71 (MQ=255) cacaTTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAAc < 1:1671658/70‑1 (MQ=255) cacaTTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAAc > 1:2302119/1‑70 (MQ=255) ttCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGa > 1:378731/1‑71 (MQ=255) ctctGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAGCGAGGATg > 1:3202735/1‑71 (MQ=255) gTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGcc < 1:2168022/71‑1 (MQ=255) gTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGcc < 1:3688723/71‑1 (MQ=255) aTCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAg > 1:1650108/1‑71 (MQ=255) tCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGa > 1:1794973/1‑61 (MQ=255) tCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGa < 1:3911819/61‑1 (MQ=255) ccGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGaa > 1:1914132/1‑53 (MQ=255) gACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGa < 1:3269600/56‑1 (MQ=255) cAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAg < 1:2812726/48‑1 (MQ=255) cAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAg < 1:3898115/48‑1 (MQ=255) aaaaGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATg < 1:773735/71‑1 (MQ=255) aaaaGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATg < 1:3057957/71‑1 (MQ=255) aaaGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAGCGAg < 1:2468712/46‑1 (MQ=255) aaaGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAGCGAg < 1:2548850/46‑1 (MQ=255) aaaGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAGCGAg < 1:3060550/46‑1 (MQ=255) aaaGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGa < 1:1315300/71‑1 (MQ=255) gCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAGCGAGGATg > 1:1888453/1‑47 (MQ=255) gCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACt > 1:3432119/1‑71 (MQ=255) ccTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTg < 1:2509125/71‑1 (MQ=255) tctGACTTGTAACAAAAACAAAGATAAAAATGAGAGCGAg < 1:1102464/40‑1 (MQ=255) tctGACTTGTAACAAAAACAAAGATAAAAATGAGAGCGAg < 1:2081320/40‑1 (MQ=255) tctGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGGTGAGaa < 1:4865/60‑1 (MQ=255) tctGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGaa < 1:2733830/60‑1 (MQ=255) tctGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGaa < 1:10099/60‑1 (MQ=255) tctGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGaa < 1:4062042/60‑1 (MQ=255) tctGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGaa < 1:2172397/60‑1 (MQ=255) tctGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGaa < 1:2279721/60‑1 (MQ=255) tctGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGaa < 1:2377353/60‑1 (MQ=255) tctGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGaa < 1:2918660/60‑1 (MQ=255) tGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAg > 1:1152407/1‑38 (MQ=255) tGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATg > 1:3970633/1‑71 (MQ=255) aCTTGTAACAAAAACAAAGATAAAAATGAGAGCGAGGATGTTGCCAGTGCTGAGAATATGAACTGGa > 1:360891/1‑67 (MQ=255) aCTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATg > 1:418927/1‑40 (MQ=255) aCTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTg < 1:4055585/65‑1 (MQ=255) aCTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTg < 1:112929/65‑1 (MQ=255) aCTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTg < 1:2113808/65‑1 (MQ=255) aCTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTg < 1:1260321/65‑1 (MQ=255) gTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCa > 1:3811652/1‑71 (MQ=255) acaaaaacaaaGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTg > 1:3138446/1‑71 (MQ=255) acaaaaacaaaGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTg > 1:308638/1‑71 (MQ=255) acaaaaacaaaGATAAAAATGACAACGAGGATGTTGca > 1:2654417/1‑37 (MQ=39) aaaaacaaaGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCa < 1:2283303/62‑1 (MQ=255) aaaaacaaaGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCa < 1:696829/62‑1 (MQ=255) aaacaaaGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATg < 1:802690/49‑1 (MQ=255) aaacaaaGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATg < 1:845163/49‑1 (MQ=255) aacaaaGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCa > 1:317780/1‑59 (MQ=255) gATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGa > 1:788476/1‑49 (MQ=255) gATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGa > 1:801163/1‑49 (MQ=255) tGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAg < 1:2532883/71‑1 (MQ=255) agaACGAGGATGTTGCCAGAGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGa < 1:3305234/71‑1 (MQ=255) gaACGAGGATGTTGCCAGTGCTGAGAATATGAACTg < 1:2285944/36‑1 (MQ=255) | TCCACATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGA > W3110S.gb/237656‑237793 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |