Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,359,906 T→C 31.1% M285T (ATG→ACG)  gltD → glutamate synthase, 4Fe‑4S protein, small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,359,9060TC31.1% 79.6 / 50.6 61M285T (ATG→ACG) gltDglutamate synthase, 4Fe‑4S protein, small subunit
Reads supporting (aligned to +/- strand):  ref base T (19/23);  new base C (10/9);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGC  >  W3110S.gb/3359837‑3359976
                                                                     |                                                                      
cGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATg                                                                       <  1:2019093/71‑1 (MQ=255)
  ttCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGa                                                                     <  1:2063136/71‑1 (MQ=255)
    ccTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAg                                                                   >  1:209694/1‑71 (MQ=255)
    ccTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAg                                                                   >  1:2792485/1‑71 (MQ=255)
        aTCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCa                                                                <  1:2854687/70‑1 (MQ=255)
           gCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAgg                                                                  >  1:549630/1‑65 (MQ=255)
           gCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAgg                                                                  >  1:551570/1‑65 (MQ=255)
           gCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAgg                                                                  >  1:850876/1‑65 (MQ=255)
           gCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAAcg                                                            >  1:381256/1‑71 (MQ=255)
           gCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAAcg                                                            >  1:2653024/1‑71 (MQ=255)
              aaCACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCa                                                                <  1:1426464/64‑1 (MQ=255)
              aaCACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCAAAcgcg                                                          >  1:3142094/1‑70 (MQ=255)
              aaCACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCAAACGCgt                                                         >  1:1853658/1‑71 (MQ=255)
               acacCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCgtg                                                        <  1:1597132/71‑1 (MQ=255)
                cacCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCaaa                                                              >  1:3015783/1‑64 (MQ=255)
                cacCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCaaa                                                              >  1:808930/1‑64 (MQ=255)
                 acCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAAACGTTCGTCAGCATGGAAGGCAAACGCgtggt                                                      >  1:3578555/1‑71 (MQ=255)
                  ccAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCAAACGCgtggtg                                                     >  1:2577633/1‑71 (MQ=255)
                  ccAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCAAACGCgtggtg                                                     >  1:279077/1‑71 (MQ=255)
                  ccAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCAAACGCgtggtg                                                     >  1:3351584/1‑71 (MQ=255)
                   cAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGATCCGTTCGTCAGCACGGAAGGCAAACGCGtggtgg                                                    >  1:2056288/1‑71 (MQ=255)
                   cAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAg                                                                   <  1:2868104/56‑1 (MQ=255)
                   cAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAg                                                                   <  1:2872898/56‑1 (MQ=255)
                   cAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAg                                                                   <  1:756303/56‑1 (MQ=255)
                    aaaCAGTTAATGGGCTTTGGTGAAACCCGCGACGAATCGTTCGTCAGCATGGAAGGCAAACGCGtggtgg                                                    >  1:244618/1‑70 (MQ=255)
                     aaCAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTc                                                  <  1:3109052/71‑1 (MQ=255)
                      aCAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCAAAcg                                                            <  1:2442043/60‑1 (MQ=255)
                      aCAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCAAAcg                                                            <  1:3388173/60‑1 (MQ=255)
                       cAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAAc                                                             >  1:2463995/1‑58 (MQ=255)
                        aGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCtt                                               <  1:1107620/71‑1 (MQ=255)
                          ttAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTgg                                             <  1:78261/71‑1 (MQ=255)
                            aaTGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGc                                            <  1:2554190/70‑1 (MQ=255)
                              tGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCg                                           <  1:993422/69‑1 (MQ=255)
                               gggCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCaaa                                                              >  1:1122941/1‑49 (MQ=255)
                               gggCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTc                                                  <  1:1639122/61‑1 (MQ=255)
                               gggCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGt                                         >  1:932597/1‑70 (MQ=255)
                               gggCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGt                                         >  1:2054731/1‑70 (MQ=255)
                                ggCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTgg                                       >  1:419293/1‑71 (MQ=255)
                                ggCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTg                                        >  1:926875/1‑70 (MQ=255)
                                 gCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGc                                      <  1:468269/71‑1 (MQ=255)
                                 gCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGc                                      <  1:3791792/71‑1 (MQ=255)
                                 gCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGATCCTTGGCGGTGGc                                      >  1:1273022/1‑71 (MQ=255)
                                 gCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGATCCTTGGCGGTGGc                                      >  1:1618517/1‑71 (MQ=255)
                                  cTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGc                                      >  1:493112/1‑70 (MQ=255)
                                  cTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGc                                      >  1:534336/1‑70 (MQ=255)
                                     tGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGac                                   <  1:514746/70‑1 (MQ=255)
                                     tGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGa                                    <  1:809342/69‑1 (MQ=255)
                                     tGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGa                                    <  1:2623041/69‑1 (MQ=255)
                                          aaaCCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGc                              <  1:1709750/70‑1 (MQ=255)
                                          aaaCCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGc                              <  1:2882004/70‑1 (MQ=255)
                                          aaaCCCGCGACGAACAGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGc                              <  1:2814610/70‑1 (MQ=255)
                                             cccGCGACGAACCGTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATg                          <  1:1315820/71‑1 (MQ=255)
                                             cccGCGACGAACCGTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATg                          <  1:2779676/71‑1 (MQ=255)
                                             cccGCGACGAACCGTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATg                          <  1:3102175/71‑1 (MQ=255)
                                                gcgACGAACCGTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGAc                       <  1:1812647/71‑1 (MQ=255)
                                                         cGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGTGACACTGCGATGGACTgcgtgcgt              <  1:83380/71‑1 (MQ=255)
                                                         cGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTgcgtgcgt              <  1:141345/71‑1 (MQ=255)
                                                         cGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTgcgtgcgt              <  1:1381328/71‑1 (MQ=255)
                                                             cGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGt          >  1:3487131/1‑71 (MQ=255)
                                                                 aGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGacac                                 >  1:3259855/1‑44 (MQ=255)
                                                                  gCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTc         <  1:1531466/67‑1 (MQ=255)
                                                                     tGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTgcgc  >  1:3447658/1‑71 (MQ=255)
                                                                     |                                                                      
CGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGC  >  W3110S.gb/3359837‑3359976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: