| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,360,083 | T→C | 40.0% | F344S (TTC→TCC) | gltD → | glutamate synthase, 4Fe‑4S protein, small subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,360,083 | 0 | T | C | 40.0% | 14.6 / 32.4 | 30 | F344S (TTC→TCC) | gltD | glutamate synthase, 4Fe‑4S protein, small subunit |
| Reads supporting (aligned to +/- strand): ref base T (9/9); new base C (9/3); total (18/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAATGGTGC > W3110S.gb/3360014‑3360148 | atgaAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTc < 1:3545157/71‑1 (MQ=255) gaAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATCCaa > 1:3965760/1‑71 (MQ=255) gaAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATCCaa > 1:1706603/1‑71 (MQ=255) gaAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATCCaa > 1:2918227/1‑71 (MQ=255) tGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAAc > 1:1309806/1‑62 (MQ=255) gCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATCCaa > 1:2967732/1‑60 (MQ=255) cgccgcGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATCCAACGTCCAGCCGCTGGGTATTGa < 1:822984/71‑1 (MQ=255) gcgAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTg < 1:2077514/71‑1 (MQ=255) aGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATCCAACGTCCAGCCGCTGGGTATTg < 1:2352106/62‑1 (MQ=255) gAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATCCAACGTCCAGCCGCTGGGTATTGaa > 1:1889779/1‑61 (MQ=255) gAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATCCAACGTCCAGCCGCTGGGTATTGaa > 1:288282/1‑61 (MQ=255) aaaaaCGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTaa > 1:3482376/1‑71 (MQ=255) cgcgcgGGAAGAAGGCGTAGAGTTCAAATCCAACGTCCAGCCGCTGGGTAt > 1:1736190/1‑51 (MQ=255) aagaagGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAgc > 1:1390028/1‑71 (MQ=255) aagaagGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAgc > 1:1584438/1‑71 (MQ=255) tAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGa < 1:278734/37‑1 (MQ=255) tAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGa < 1:1044773/37‑1 (MQ=255) tAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCaaa < 1:2734526/56‑1 (MQ=255) tAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCaaa < 1:2967049/56‑1 (MQ=255) tAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCaaa < 1:381569/56‑1 (MQ=255) tAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCa < 1:2123141/54‑1 (MQ=255) gagTTCAAATTCAACGTCCAGCCGCTGGGTATTGaa > 1:1436404/1‑36 (MQ=255) gagTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAgcgg > 1:2272121/1‑62 (MQ=255) gagTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAAt < 1:3718964/71‑1 (MQ=255) gagTTCAAATCCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAAt > 1:3447861/1‑71 (MQ=255) gagTTCAAATCCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAAt < 1:1704859/71‑1 (MQ=255) ttCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAATGGt > 1:1583358/1‑71 (MQ=255) cAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTaa > 1:759358/1‑42 (MQ=255) cAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAATGGTGc > 1:848089/1‑71 (MQ=255) cAAATCCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAATGGTGc > 1:3989245/1‑71 (MQ=255) | ATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCGTAGAGTTCAAATTCAACGTCCAGCCGCTGGGTATTGAAGTGAACGGTAACGGCAAAGTCAGCGGCGTAAAAATGGTGC > W3110S.gb/3360014‑3360148 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |