| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,383,928 | C→T | 17.5% | A66T (GCG→ACG) | mdh ← | malate dehydrogenase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,383,928 | 0 | C | T | 17.5% | 95.1 / 12.5 | 40 | A66T (GCG→ACG) | mdh | malate dehydrogenase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (16/17); new base T (5/2); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGA > W3110S.gb/3383862‑3383993 | aaCGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGa < 1:391415/71‑1 (MQ=255) aTCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCg > 1:2110544/1‑71 (MQ=255) cccGGTTTACGCGCTACGCCTGCAGAGTTAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCtt < 1:2504362/70‑1 (MQ=255) cccGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCtt < 1:1811605/70‑1 (MQ=255) ttACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCa > 1:764195/1‑69 (MQ=255) ttACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAg > 1:1037436/1‑70 (MQ=255) cgcgCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGaaaa < 1:478050/71‑1 (MQ=255) cAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt < 1:3871908/70‑1 (MQ=255) cAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt > 1:2362630/1‑70 (MQ=255) cAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt < 1:2397310/70‑1 (MQ=255) gaTAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCAcag > 1:3182168/1‑71 (MQ=255) gaTAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTAACCAGAAAAACCTTTGATTTTCAtag > 1:1460181/1‑71 (MQ=255) tAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGaaaa < 1:2762813/52‑1 (MQ=255) tAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt < 1:1389328/64‑1 (MQ=255) tAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt < 1:2267155/64‑1 (MQ=255) aGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGaaaaag > 1:3782295/1‑51 (MQ=255) aGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCAcagcag < 1:1603031/70‑1 (MQ=255) aGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCAcagcag > 1:3761619/1‑70 (MQ=255) aGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCAcagca > 1:2589668/1‑69 (MQ=255) aCGACATCTGCGACTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCAcagcag > 1:1253953/1‑67 (MQ=255) gACATCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGa < 1:2377814/71‑1 (MQ=255) tCTGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGa < 1:874035/67‑1 (MQ=255) tCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGAtttt < 1:3428646/53‑1 (MQ=255) tCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACC‑TTGATTTTCAcagcag < 1:1451004/60‑1 (MQ=255) tGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATgg > 1:1893627/1‑70 (MQ=255) tGCGCCTTCCAGCGTCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGc > 1:407915/1‑71 (MQ=255) tGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGaaaa > 1:1779860/1‑39 (MQ=255) tGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCAcagca > 1:2032142/1‑58 (MQ=255) tGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGc > 1:3467246/1‑71 (MQ=255) cgcCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATg < 1:828033/67‑1 (MQ=255) ccTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAg < 1:3989198/71‑1 (MQ=255) ccTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAg < 1:461239/71‑1 (MQ=255) cTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCAcagc < 1:3248863/52‑1 (MQ=255) ccAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATc > 1:1785273/1‑70 (MQ=255) ccAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATc > 1:2488715/1‑70 (MQ=255) cAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGa < 1:3998224/71‑1 (MQ=255) aGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGa > 1:2594816/1‑70 (MQ=255) aGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGa > 1:53502/1‑70 (MQ=255) aGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGa > 1:3129048/1‑70 (MQ=255) aGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGa > 1:1142770/1‑70 (MQ=255) | AACGATCCATACCCGGTTTACGCGCTACGCCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGA > W3110S.gb/3383862‑3383993 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |