| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,404,737 | C→T | 16.1% | intergenic (+424/‑554) | yhdH → / → accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,404,737 | 0 | C | T | 16.1% | 141.9 / 17.9 | 56 | intergenic (+424/‑554) | yhdH/accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Reads supporting (aligned to +/- strand): ref base C (27/20); new base T (4/5); total (31/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
CGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGA > W3110S.gb/3404668‑3404806 | cGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGc > 1:3338398/1‑70 (MQ=255)cGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGc > 1:202119/1‑70 (MQ=255) gTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAAc < 1:3408652/71‑1 (MQ=255) tAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCg > 1:2937572/1‑71 (MQ=255) tAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCg > 1:1678698/1‑71 (MQ=255) ttttGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGc < 1:3761111/63‑1 (MQ=255) ttttGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTg < 1:1059466/71‑1 (MQ=255) ttGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGTAACCGCTGCg > 1:3166642/1‑71 (MQ=255) ttGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGTAACCGCTGCg > 1:1873927/1‑71 (MQ=255) ttGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCg > 1:658728/1‑66 (MQ=255) gTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTa < 1:2443722/69‑1 (MQ=255) ttCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAAcc < 1:2272549/50‑1 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGTAACCGCTGCGTAATGTCGTCTATCTTg > 1:3156719/1‑71 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGTAACCGCTGCGTAATGTCGTCTATCTTg > 1:2400088/1‑71 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCaa > 1:732222/1‑46 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCaa > 1:2804301/1‑46 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCt > 1:2789870/1‑65 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTg > 1:378108/1‑71 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTg > 1:2793151/1‑71 (MQ=255) cacaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTg > 1:567127/1‑71 (MQ=255) acaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTg > 1:1353363/1‑70 (MQ=255) acaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTg > 1:3247644/1‑70 (MQ=255) acaTCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGt > 1:1299917/1‑71 (MQ=255) tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGc < 1:3345533/46‑1 (MQ=255) tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGc < 1:2710361/46‑1 (MQ=255) tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGc < 1:1659132/46‑1 (MQ=255) tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgc > 1:3491489/1‑71 (MQ=255) tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgc > 1:1366714/1‑71 (MQ=255) cTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgc > 1:2147083/1‑70 (MQ=255) cTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCATGTcgc > 1:3935194/1‑70 (MQ=255) tATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCt > 1:187522/1‑45 (MQ=255) tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGTAACCGCTGCGTAATGTCGTCTATCTTGTCGCGAt < 1:3555503/70‑1 (MQ=255) tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgc < 1:2186868/67‑1 (MQ=255) tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGa < 1:2659521/69‑1 (MQ=255) tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGa < 1:3032566/69‑1 (MQ=255) cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc < 1:3272214/70‑1 (MQ=255) cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCt > 1:502278/1‑71 (MQ=255) gATGCAGTGAAAATTGGGTAATCCCCAGTAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCAt < 1:40491/71‑1 (MQ=255) tGCAGTGAAAATTGGGTAATCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATcc > 1:2717712/1‑71 (MQ=255) aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCa > 1:30359/1‑61 (MQ=255) gggTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTtgt > 1:2416443/1‑71 (MQ=255) gggTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTtg < 1:65567/70‑1 (MQ=255) ggTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTtgtg < 1:2329747/71‑1 (MQ=255) ggTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTtgtg < 1:1809318/71‑1 (MQ=255) aaTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTc > 1:1264035/1‑71 (MQ=255) aTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCt > 1:1308409/1‑71 (MQ=255) aTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCt > 1:2431011/1‑71 (MQ=255) tCCCCAGTAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc < 1:444363/43‑1 (MQ=255) tCCCCAGTAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc < 1:1781928/43‑1 (MQ=255) tCCCCAGTAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc < 1:1823380/43‑1 (MQ=255) tCCCCAGCAACCACTGCGTAATGTCGTCTATCTTGTCGCTATCCTGGCATCCCTACATTAttt < 1:1658287/63‑1 (MQ=38) cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg < 1:1155413/71‑1 (MQ=255) cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg < 1:3551241/71‑1 (MQ=255) cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg < 1:2726719/71‑1 (MQ=255) cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgt > 1:2148881/1‑70 (MQ=255) cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtg > 1:822284/1‑69 (MQ=255) cAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGaa < 1:2192863/71‑1 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATcc > 1:3450035/1‑46 (MQ=255) gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGa < 1:196650/71‑1 (MQ=255) | CGGTTAATTTTGCGTGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGA > W3110S.gb/3404668‑3404806 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |