| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,404,829 | C→G | 31.3% | intergenic (+516/‑462) | yhdH → / → accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,404,829 | 0 | C | G | 31.3% | 81.6 / 50.2 | 67 | intergenic (+516/‑462) | yhdH/accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Reads supporting (aligned to +/- strand): ref base C (26/20); new base G (10/11); total (36/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAA > W3110S.gb/3404761‑3404895 | cTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCtt > 1:3284454/1‑71 (MQ=255) gTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCg > 1:779181/1‑70 (MQ=255) tCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGc < 1:2519527/70‑1 (MQ=255) gggATCCTGGCATCCCTACATTTTTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAgg < 1:284931/69‑1 (MQ=255) cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAgg < 1:541849/70‑1 (MQ=255) aTCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct < 1:960671/71‑1 (MQ=255) aTCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct < 1:407808/71‑1 (MQ=255) cTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGggctgg < 1:3950550/70‑1 (MQ=255) cTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGggctgg < 1:3033417/70‑1 (MQ=255) ggCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgctt > 1:110712/1‑71 (MQ=255) ggCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgctt > 1:2342106/1‑71 (MQ=255) gCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttt < 1:2612832/71‑1 (MQ=255) ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagc < 1:2450972/71‑1 (MQ=255) ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagc > 1:2947892/1‑71 (MQ=255) ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagc > 1:2761726/1‑71 (MQ=255) cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagctttt > 1:1134486/1‑71 (MQ=255) cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagctttt > 1:1763465/1‑71 (MQ=255) cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagctt < 1:2929794/69‑1 (MQ=255) cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAg > 1:630452/1‑52 (MQ=255) cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt > 1:1025237/1‑71 (MQ=255) cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt > 1:2814651/1‑71 (MQ=255) cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt > 1:533322/1‑71 (MQ=255) attattTGTGGTCTGTGAAGAATATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt > 1:553761/1‑70 (MQ=255) ttattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagcttttca > 1:2700442/1‑71 (MQ=255) tattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGggctg > 1:1449926/1‑54 (MQ=255) tattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagcttttca > 1:406749/1‑70 (MQ=255) attTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCAc < 1:2461004/70‑1 (MQ=255) tgtgGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCtt > 1:326326/1‑70 (MQ=255) gTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGt < 1:3941537/70‑1 (MQ=255) gTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGt < 1:679963/70‑1 (MQ=255) tCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagcttttca < 1:3128216/61‑1 (MQ=255) tCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGt < 1:3724326/69‑1 (MQ=255) tgtgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagct > 1:2441564/1‑54 (MQ=255) tgtgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGt > 1:432170/1‑67 (MQ=255) tgtgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTtat > 1:2938370/1‑70 (MQ=255) tgAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTtat > 1:1186297/1‑68 (MQ=255) tgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTtata < 1:1660773/69‑1 (MQ=255) tgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTtata < 1:1533662/69‑1 (MQ=255) tgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTtat < 1:702399/68‑1 (MQ=255) aGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctggct < 1:1328749/41‑1 (MQ=255) ttATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagct < 1:117550/46‑1 (MQ=255) ttATCTCATTGCAGCCCCTCATCTTCGCAGGGCAGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGtt > 1:2351032/1‑70 (MQ=255) ttATCTCATTGCAGCCCCTCATCTTCGCAGGGCAGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGt > 1:1273267/1‑69 (MQ=255) aTCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagcttt > 1:1634419/1‑46 (MQ=255) aTCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagcttt > 1:3894893/1‑46 (MQ=255) aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:3310243/68‑1 (MQ=255) aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:2370121/68‑1 (MQ=255) aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:3047169/68‑1 (MQ=255) aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgat < 1:3255104/71‑1 (MQ=255) tGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatga > 1:2836976/1‑71 (MQ=255) gCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatgat > 1:3595236/1‑71 (MQ=255) gCAGCCCCTCATCTTCGAAGGGCTGGCTTTTCAGCTTTTCACATTACGTTATAAGAAGTTCCGTCgatga > 1:3135594/1‑70 (MQ=255) cAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatgat < 1:3052343/70‑1 (MQ=255) cAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatgat < 1:1367316/70‑1 (MQ=255) cccTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgat < 1:3852411/63‑1 (MQ=255) cccTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgat < 1:2527212/63‑1 (MQ=255) cccTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgat < 1:1441325/63‑1 (MQ=255) tCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatgat < 1:3962385/63‑1 (MQ=255) tCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatgat < 1:3229720/63‑1 (MQ=255) tCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATTATGGCGCTaa > 1:458343/1‑71 (MQ=255) cATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATgg > 1:157287/1‑64 (MQ=255) cATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATgg > 1:741996/1‑64 (MQ=255) cATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACATTACGTTATAAGAAGTTCCGTCGATGATgg > 1:1907927/1‑64 (MQ=255) aTCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaagaag > 1:2348530/1‑48 (MQ=255) aTCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaagaag > 1:3739735/1‑48 (MQ=255) tCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaagaag > 1:2337159/1‑47 (MQ=255) tCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaagaag > 1:194821/1‑47 (MQ=255) | CTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAA > W3110S.gb/3404761‑3404895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |