| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,442,070 | A→G | 18.4% | T497A (ACG→GCG) | thiC → | thiamin (pyrimidine moiety) biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,442,070 | 0 | A | G | 18.4% | 106.1 / 19.4 | 49 | T497A (ACG→GCG) | thiC | thiamin (pyrimidine moiety) biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base A (19/21); new base G (2/7); total (21/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 | |||||||||||
ACCACTTCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATT > W3110S.gb/3442002‑3442138 | aCCACTTCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACg > 1:1135255/1‑71 (MQ=255) tCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:194183/1‑69 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:2425015/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:3422977/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:3487759/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:3706619/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:3758739/1‑68 (MQ=255) cACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCaa > 1:4031233/1‑68 (MQ=255) gTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGc < 1:1495588/71‑1 (MQ=255) gTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGc < 1:2464096/71‑1 (MQ=255) tCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCa < 1:3371667/71‑1 (MQ=255) tCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCa < 1:2957858/71‑1 (MQ=255) gATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTg > 1:2507763/1‑70 (MQ=255) aTTGGTGCGGCGATGATTGGCTGGTTTGGTTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTggg < 1:3788940/71‑1 (MQ=255) aTTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTggg > 1:3732918/1‑71 (MQ=255) aTTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTggg < 1:1366099/71‑1 (MQ=255) aTTGGTGCGGCGATGATTGGCTGGTTTGGCT‑‑GCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTc > 1:1249628/1‑71 (MQ=255) ggTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCt > 1:1400025/1‑71 (MQ=255) ggTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCt > 1:915467/1‑71 (MQ=255) atgatTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATa > 1:2094733/1‑70 (MQ=255) atgatTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATa > 1:2009242/1‑70 (MQ=255) atgatTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATa > 1:2004602/1‑70 (MQ=255) ttGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:398588/71‑1 (MQ=255) tGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCAt > 1:2644865/1‑49 (MQ=255) cTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATg < 1:270778/71‑1 (MQ=255) gTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAagag < 1:1389619/40‑1 (MQ=255) gTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAagag < 1:3866531/40‑1 (MQ=255) gTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAagag < 1:2178243/40‑1 (MQ=255) gTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAagag < 1:1859788/40‑1 (MQ=255) ggCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGc < 1:1622368/70‑1 (MQ=255) ggCTGCGCGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGc < 1:626410/70‑1 (MQ=255) gcgcGATGCTCTGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGc < 1:1834339/66‑1 (MQ=255) tGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGccc < 1:565884/41‑1 (MQ=255) tctGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGtt < 1:820362/53‑1 (MQ=255) tctGTTACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGtt < 1:756229/53‑1 (MQ=255) ttACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtata < 1:1095693/71‑1 (MQ=255) ttACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaag < 1:2898300/44‑1 (MQ=255) ttACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAaagaa < 1:4004442/43‑1 (MQ=255) tACGTAGCGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtatt < 1:2791400/70‑2 (MQ=255) aCGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAagaaga > 1:261041/1‑43 (MQ=255) aCGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAagaaga > 1:1423003/1‑43 (MQ=255) aCGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAagaaga > 1:2056239/1‑43 (MQ=255) aCGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCtat > 1:4045430/1‑68 (MQ=255) tAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTa < 1:1475069/45‑1 (MQ=255) tAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATaa < 1:2574235/67‑1 (MQ=255) tAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATaa < 1:1978463/67‑1 (MQ=255) tAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGAtt < 1:2709178/71‑1 (MQ=255) tAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGAtt < 1:4041536/71‑1 (MQ=255) tAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGAtt < 1:502046/71‑1 (MQ=255) | ACCACTTCACGTCGGGGATTGGTGCGGCGATGATTGGCTGGTTTGGCTGCGCGATGCTCTGTTACGTAACGCCAAAAGAGCATCTGGGTCTGCCCAATAAAGAAGATGTTAAGCAGGGGCTTATCACCTATAAGATT > W3110S.gb/3442002‑3442138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |