| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,449,319 | A→G | 35.7% | G671G (GGT→GGC) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,449,319 | 0 | A | G | 35.7% | 20.4 / 25.8 | 28 | G671G (GGT→GGC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base A (9/9); new base G (5/5); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01 | |||||||||||
GATACACGATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGACCAGACTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTC > W3110S.gb/3449251‑3449385 | gATACACGATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTaccagac < 1:3117552/70‑1 (MQ=255)gATACACGATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTaccagac < 1:4029270/70‑1 (MQ=255)gATACACGATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTaccagac < 1:2417672/70‑1 (MQ=255)gATACACGATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTaccagac < 1:2369688/70‑1 (MQ=255) aTACACGATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTaccaggcca < 1:3509271/71‑1 (MQ=255) aTACACGATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTaccaggcca < 1:3891768/71‑1 (MQ=255) aTACACGATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTaccagacca < 1:1182213/71‑1 (MQ=255) aTACACGATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTaccagacca < 1:3616398/71‑1 (MQ=255) cacGATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTAccagaccaga < 1:1751692/70‑1 (MQ=255) gATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGGCCAGACTgg < 1:3953347/71‑1 (MQ=255) gATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGACCAGACTgg < 1:347502/71‑1 (MQ=255) gATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGACCAGACTgg < 1:3938052/71‑1 (MQ=255) gATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGACCAGACTgg < 1:1488937/71‑1 (MQ=255) gATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGACCAGACTgg < 1:2040320/71‑1 (MQ=255) cGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGACCAGACTGGAAc > 1:1868180/1‑71 (MQ=255) cGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGACCAGACTGGAAc > 1:3222532/1‑71 (MQ=255) cGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGACCAGACTGGAAc > 1:3971353/1‑71 (MQ=255) cgccgcAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTAccagaccaga > 1:2386164/1‑60 (MQ=255) cgccgcAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGGCCAGACTGGAACTGCt > 1:3687437/1‑71 (MQ=255) cgccgcAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGGCCAGACTGGAACTGCt > 1:3674838/1‑71 (MQ=255) cAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGGCCAGACTGGAACTGCTCCTGa < 1:2585574/71‑1 (MQ=255) cAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGGCCAGACTGGAACTGCTCCTGa < 1:998081/71‑1 (MQ=255) cAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGGCCAGACTGGAACTGCTCCTGa < 1:3040611/71‑1 (MQ=255) cAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGGCCAGACTGGAACTGCTCCTGa < 1:1846461/71‑1 (MQ=255) tatCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGACCAGACTGGAACTGCTCCTGAATTTCAGc > 1:3641740/1‑70 (MQ=255) tatCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGACCAGACTGGAACTGCTCCTGAATTTCAGc > 1:587005/1‑70 (MQ=255) tatCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGACCAGACTGGAACTGCTCCTGAATTTCAGCa < 1:1633398/71‑1 (MQ=255) atCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTAccagaccaga > 1:579943/1‑45 (MQ=255) atCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGACCAGACTGGAACTGCTCCTGAATTTCAGCaa > 1:1223363/1‑71 (MQ=255) gATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGGCCAGACTGGAACTGCTCCTGAATTTCAGCAActt > 1:3633140/1‑71 (MQ=255) cTTTGTTGTAGCGTTCGCCCGCAGTTACCAGACCAGACTGGAACTGCTCCTGAATTTCAGCAActtctgct < 1:289192/71‑1 (MQ=255) tttGTTGTAGCGTTCGCCCGCAGTTACCAGGCCAGACTGGAACTGCTCCTGAATTTCAGCaa > 1:1813902/1‑62 (MQ=255) ttgttgTAGCGTTCGCCCGCAGTTACCAGACCAGACTGGAACTGCTCCTGAATTTCAGCAActtctgcttc > 1:3637196/1‑71 (MQ=255) tgttgtAGCGTTCGCCCGCAGTTACCAGGCCAGACTGGAACTGCTCCTGAATTTCAGCAAc > 1:3026628/1‑61 (MQ=255) tAGCGTTCGCCCGCAGTTACCAGACCAGACTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCt < 1:1593159/70‑1 (MQ=255) cGCCCGCAGTTACCAGACCAGACTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGga > 1:3548816/1‑67 (MQ=255) gTTACCAGACCAGACTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCg < 1:2997850/57‑1 (MQ=255) ttACCAGACCAGACTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCgtgt < 1:2915905/71‑1 (MQ=255) tACCAGACCAGACTGGAACTGCTCCTGAATTTCAGCAActtctgctt > 1:83047/1‑47 (MQ=255) accaggccagACTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGtt < 1:2774611/70‑1 (MQ=255) accaggccagACTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGtt < 1:1742794/70‑1 (MQ=255) accaggacagACTGGAACTGCTCCTGAATTTCAGCAActt > 1:1768190/1‑40 (MQ=38) accagaccagACTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGttt > 1:245887/1‑71 (MQ=255) accagaccagACTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGttt > 1:684726/1‑71 (MQ=255) accagaccagACTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGtt < 1:2774310/70‑1 (MQ=255) accagaccagACTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGtt < 1:574375/70‑1 (MQ=255) ccagaccagaCTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTttc < 1:2569899/71‑1 (MQ=255) ccagaccagaCTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTttc < 1:1605155/71‑1 (MQ=255) | GATACACGATCGTTCGCCGCAGCCCAGATATCGATAACTTTGTTGTAGCGTTCGCCCGCAGTTACCAGACCAGACTGGAACTGCTCCTGAATTTCAGCAACTTCTGCTTCTGCCTCGGAGATGATTTCGTGTTTC > W3110S.gb/3449251‑3449385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |