| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,488,502 | C→T | 41.3% | L47L (CTG→TTG) | yijO → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,488,502 | 0 | C | T | 41.3% | 24.7 / 53.3 | 46 | L47L (CTG→TTG) | yijO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (6/21); new base T (9/10); total (15/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
CCAGCAGTAACGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGATGTGCTGTACGTTCC > W3110S.gb/3488437‑3488569 | ccAGCAGTAACGGCCCGTTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGa < 1:1595587/70‑1 (MQ=255)ccAGCAGTAACGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGa < 1:1692941/70‑1 (MQ=255) aCGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTCAGttt > 1:1095830/1‑71 (MQ=255) gCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt < 1:1441547/71‑1 (MQ=255) tGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATAcc < 1:1980146/71‑1 (MQ=255) tGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATAcc < 1:2609139/71‑1 (MQ=255) tGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATAcc < 1:874319/71‑1 (MQ=255) cccGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCgg < 1:2216586/71‑1 (MQ=255) aTCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGa < 1:214912/61‑1 (MQ=255) aTCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGa < 1:3503994/61‑1 (MQ=255) aTCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGa < 1:1582518/61‑1 (MQ=255) cTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTg > 1:2539096/1‑70 (MQ=255) cTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGCAAGGTGAGTTTGTTGATACCGGCGCTg > 1:1470109/1‑70 (MQ=255) cTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTg > 1:863216/1‑70 (MQ=255) gCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt < 1:1533269/54‑1 (MQ=255) gCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt < 1:914299/54‑1 (MQ=255) gCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTttgtt < 1:2901331/54‑1 (MQ=255) cGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCa < 1:2951246/71‑1 (MQ=255) cGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCa < 1:3762323/71‑1 (MQ=255) gTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGa > 1:1392476/1‑54 (MQ=255) gTATCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGa > 1:2910255/1‑54 (MQ=255) tATCAGGTCGATTTTCCACGTCTGGAAATTGTGCTGGa < 1:2324352/38‑1 (MQ=255) tCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGaa < 1:1732881/37‑1 (MQ=255) tCAGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt > 1:1435568/1‑71 (MQ=255) tCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGttt < 1:3717328/46‑1 (MQ=255) tCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGttt < 1:1302706/46‑1 (MQ=255) aGGTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt > 1:2318188/1‑69 (MQ=255) ggTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAg < 1:1945673/71‑1 (MQ=255) gTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt < 1:217149/67‑1 (MQ=255) gTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt < 1:3377181/67‑1 (MQ=255) gTCGATTTTCCACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGt < 1:3381655/67‑1 (MQ=255) gTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAg < 1:2084015/39‑1 (MQ=255) gTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAg < 1:3581862/39‑1 (MQ=255) cGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGa > 1:1236383/1‑45 (MQ=255) cGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGa > 1:3400740/1‑45 (MQ=255) cGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGa > 1:2384398/1‑45 (MQ=255) cGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGa > 1:14291/1‑45 (MQ=255) cGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTc > 1:94165/1‑71 (MQ=255) tCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGc < 1:2046589/70‑1 (MQ=255) ccACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCg < 1:3308628/70‑1 (MQ=255) ccACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCg < 1:2567763/70‑1 (MQ=255) ccACGGCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCg < 1:2140709/70‑1 (MQ=255) aCGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGa < 1:3491534/36‑1 (MQ=255) gCTGGAAATTGTGTTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGAtgt > 1:462962/1‑69 (MQ=255) gCTGGAAATTGTGTCGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGAtgtg > 1:1700679/1‑70 (MQ=255) tgCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGATGTGCTGTACGTTcc < 1:1809527/70‑1 (MQ=255) | CCAGCAGTAACGGCCCGGTGCCCGATCTTGCGTATCAGGTCGATTTTCCACGGCTGGAAATTGTGCTGGAAGGTGAGTTTGTTGATACCGGCGCTGGAGCAACGTTAGTTCCCGGCGATGTGCTGTACGTTCC > W3110S.gb/3488437‑3488569 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |