| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,497,705 | T→G | 7.9% | R20R (CGT→CGG) | gldA → | glycerol dehydrogenase, NAD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,497,705 | 0 | T | G | 7.9% | 151.1 / 9.5 | 63 | R20R (CGT→CGG) | gldA | glycerol dehydrogenase, NAD |
| Reads supporting (aligned to +/- strand): ref base T (36/22); new base G (2/3); total (38/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
AGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTT > W3110S.gb/3497639‑3497774 | aGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGAGCTGATGTGATTAATCGTCTgg > 1:3124788/1‑71 (MQ=255) aaTTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCg < 1:1492195/71‑1 (MQ=255) aaTTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCg < 1:3115406/71‑1 (MQ=255) aTGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATa > 1:2104707/1‑71 (MQ=255) aTGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATa > 1:1479889/1‑71 (MQ=255) gACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCt > 1:1632730/1‑71 (MQ=255) ccGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTg > 1:509869/1‑70 (MQ=255) cGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGaa < 1:2465315/71‑1 (MQ=255) gCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAg > 1:916952/1‑71 (MQ=255) gCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAg > 1:3224173/1‑71 (MQ=255) cATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGa > 1:2602009/1‑60 (MQ=255) attattCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGcc > 1:2201414/1‑71 (MQ=255) attCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGaa > 1:1288292/1‑57 (MQ=255) attCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCgat > 1:1489032/1‑69 (MQ=255) attCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCt > 1:157596/1‑71 (MQ=255) tCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTgg > 1:2738928/1‑71 (MQ=255) aaTCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTgg > 1:546323/1‑69 (MQ=255) tCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAg > 1:2835931/1‑70 (MQ=255) cACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGaa > 1:661925/1‑50 (MQ=255) cGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGc < 1:707801/71‑1 (MQ=255) gTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTgg > 1:2576314/1‑71 (MQ=255) tAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt < 1:2655651/71‑1 (MQ=255) tAAATACATCCAGGGCGCTGATGTGATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt < 1:3583212/71‑1 (MQ=255) aaTACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt > 1:689796/1‑69 (MQ=255) aaTACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt > 1:1372592/1‑69 (MQ=255) aCATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtg > 1:1815463/1‑71 (MQ=255) cATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtg < 1:3146154/70‑1 (MQ=255) cATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtg < 1:3829506/70‑1 (MQ=255) cATACAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtgg > 1:730411/1‑71 (MQ=255) tCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGt < 1:2952952/63‑1 (MQ=255) tCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:2602016/67‑1 (MQ=255) tCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAAAGCTGGt < 1:3618731/63‑1 (MQ=255) cAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggtg > 1:454512/1‑69 (MQ=255) cAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggtg > 1:3942665/1‑69 (MQ=255) aGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTggtgggt < 1:3335724/71‑1 (MQ=255) ggCGCTGATGTGATTAATCGTCTGGGCGAATACCTg > 1:3172695/1‑36 (MQ=255) ggCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAg > 1:1229957/1‑61 (MQ=255) cTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:2199926/58‑1 (MQ=255) cTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:428980/58‑1 (MQ=255) gATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAtt > 1:485440/1‑71 (MQ=255) gATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAAt > 1:247017/1‑70 (MQ=255) gtgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGa < 1:3470388/41‑1 (MQ=255) gtgATTAATCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACa < 1:2573250/64‑1 (MQ=255) tgATTAATCGTCTGGGCGAATACCTTAAGCCGCTGGCAGAACGCTGGTTAgtggt < 1:930896/55‑1 (MQ=255) tgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt < 1:2872112/55‑1 (MQ=255) tgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt < 1:2304898/55‑1 (MQ=255) tgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgtggt < 1:3703030/55‑1 (MQ=255) tgATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGt < 1:2185687/70‑1 (MQ=255) aTTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGttt > 1:46091/1‑70 (MQ=255) aTTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGttt > 1:1276756/1‑70 (MQ=255) aTTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGttt > 1:2961722/1‑70 (MQ=255) aTTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGttt > 1:2695180/1‑70 (MQ=255) aTTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGttt > 1:1394711/1‑70 (MQ=255) ttAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:951972/49‑1 (MQ=255) ttAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGttt > 1:2617900/1‑69 (MQ=255) aaTCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAgt < 1:2465344/47‑1 (MQ=255) aaTCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAgg > 1:3849954/1‑71 (MQ=255) aTCGGCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAgg < 1:2158054/70‑1 (MQ=255) cGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGtt > 1:3732285/1‑70 (MQ=255) gTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGtggtgg > 1:3726702/1‑48 (MQ=255) gTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGtttt > 1:1593958/1‑71 (MQ=255) gTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGttt < 1:1464225/70‑1 (MQ=255) gTCTGGGCGAATACCTGAAGCCGCTGCCAGAACGCTGGTTAGtggtgg > 1:3955883/1‑48 (MQ=255) | AGCAATTATGGACCGCATTATTCAATCACCGGGTAAATACATCCAGGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAGTGGTGGGTGACAAATTTGTTTTAGGTTTT > W3110S.gb/3497639‑3497774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |