| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,515,054 | A→G | 15.0% | N41D (AAC→GAC) | hslU → | molecular chaperone and ATPase component of HslUV protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,515,054 | 0 | A | G | 15.0% | 96.6 / 10.6 | 40 | N41D (AAC→GAC) | hslU | molecular chaperone and ATPase component of HslUV protease |
| Reads supporting (aligned to +/- strand): ref base A (13/21); new base G (4/2); total (17/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GGCCAGGACAACGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGGTGTCGGTAA > W3110S.gb/3514988‑3515121 | ggCCAGGACAACGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCGACg > 1:2651264/1‑70 (MQ=255)ggCCAGGACAACGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACg < 1:1317870/70‑1 (MQ=255) gCCATGACAACGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACg < 1:1091449/69‑1 (MQ=255) aCGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTgcgc < 1:770299/71‑1 (MQ=255) cGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTgcgc > 1:3380716/1‑70 (MQ=255) cAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATg < 1:2071697/71‑1 (MQ=255) cAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATg < 1:207710/71‑1 (MQ=255) cAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATg < 1:3139657/71‑1 (MQ=255) cGTTCTGTGGCGTTTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGt < 1:3353823/71‑1 (MQ=255) tgGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGaa < 1:1844971/47‑1 (MQ=255) tgGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGaa < 1:2536225/47‑1 (MQ=255) ttGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAat < 1:3571320/71‑1 (MQ=255) ttGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAat < 1:1290226/71‑1 (MQ=255) tGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGaa > 1:907330/1‑55 (MQ=255) tGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGaa > 1:402369/1‑55 (MQ=255) tGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGaa > 1:2507224/1‑55 (MQ=255) tGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAata > 1:3190206/1‑71 (MQ=255) gCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCGACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAatat > 1:3520738/1‑71 (MQ=255) gCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCGACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAata > 1:2202517/1‑70 (MQ=255) gCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAata < 1:596841/70‑1 (MQ=255) gCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAata < 1:689514/70‑1 (MQ=255) ctctGCGTAACCGCTGGCGTCGCATGCAGCTCGACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAatat < 1:3500702/70‑1 (MQ=255) cGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCtgat > 1:1817525/1‑71 (MQ=255) cGTAACCGCTGGCGTCGCATACAGCTCGACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCt < 1:4009714/68‑1 (MQ=255) tAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGaaa < 1:3255942/58‑1 (MQ=255) gCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGc > 1:933704/1‑71 (MQ=255) gCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGccc < 1:308422/69‑1 (MQ=255) gCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGccc < 1:1943939/69‑1 (MQ=255) gTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTgatgat > 1:3275411/1‑61 (MQ=255) gTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTgatgat > 1:2129737/1‑61 (MQ=255) gCATGCAGCTCGACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTgatgat > 1:2520604/1‑58 (MQ=255) gCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGgt > 1:321011/1‑71 (MQ=255) gCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGgt > 1:1195596/1‑71 (MQ=255) gCATGCAGCTCAACGAAGAGCTGAGCCATGAAGTGACCCCGAAAAATATCCTgatgat > 1:3973270/1‑58 (MQ=255) cAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGcc > 1:3051892/1‑58 (MQ=255) cTCAACGAAGAGCTGCGCCATGAAGTGACCCCGaaaaa < 1:3789991/38‑1 (MQ=255) cTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGGTGTCGGTaa < 1:3129800/71‑1 (MQ=255) cTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGGTGTCGGTaa < 1:3863521/71‑1 (MQ=255) cTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGACCGACCGGTGTCGGTaa < 1:825513/71‑1 (MQ=255) tCAACGAAGAGCTGCGCCATGAAGTGACCCCGaaaaa < 1:2289602/37‑1 (MQ=255) | GGCCAGGACAACGCCAAGCGTTCTGTGGCGATTGCTCTGCGTAACCGCTGGCGTCGCATGCAGCTCAACGAAGAGCTGCGCCATGAAGTGACCCCGAAAAATATCCTGATGATCGGCCCGACCGGTGTCGGTAA > W3110S.gb/3514988‑3515121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |