| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,560,351 | G→A | 19.1% | P62L (CCC→CTC) | rbn ← | tRNA processing exoribonuclease BN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,560,351 | 0 | G | A | 19.1% | 153.8 / 28.4 | 68 | P62L (CCC→CTC) | rbn | tRNA processing exoribonuclease BN |
| Reads supporting (aligned to +/- strand): ref base G (34/21); new base A (8/5); total (42/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01 | |||||||||||
TAGCAGGCAGAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCAAG > W3110S.gb/3560289‑3560421 | tAGCAGGCAGAAAGTTGTCAAAAATAAAGTGACGTAACTGAATGCTGATGTCGGAAAACATgaga < 1:2604191/65‑1 (MQ=255)tAGCAGGCAGAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATgaga < 1:522803/65‑1 (MQ=255) aGCAGGCAGAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAgcgg > 1:3264704/1‑71 (MQ=255) gcaggcagAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGaaa > 1:2593881/1‑65 (MQ=255) gcaggcagAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGaaa > 1:2473033/1‑65 (MQ=255) gcaggcagAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGaaa > 1:3891415/1‑65 (MQ=255) gcagAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGaaa < 1:4056210/71‑1 (MQ=255) agAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAg > 1:1012641/1‑71 (MQ=255) agAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAg > 1:2207902/1‑71 (MQ=255) agAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAg > 1:3492996/1‑71 (MQ=255) gAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAgc < 1:160778/61‑1 (MQ=255) aaaGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAgcg > 1:3630349/1‑71 (MQ=255) aaGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAgcg > 1:4001572/1‑70 (MQ=255) aaGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAgcg > 1:1693684/1‑70 (MQ=255) aaGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAgcg > 1:559211/1‑70 (MQ=255) aaGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAgcg > 1:2400676/1‑70 (MQ=255) aaGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAgcg > 1:2849235/1‑70 (MQ=255) aGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGaaaa > 1:2885621/1‑66 (MQ=255) ggCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCaaaaa > 1:3621216/1‑71 (MQ=255) ggCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCaaaaa > 1:3046442/1‑71 (MQ=255) gCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaac < 1:302412/71‑1 (MQ=255) gCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCAAAaac < 1:425503/71‑1 (MQ=255) gCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCAAAaac < 1:3259071/71‑1 (MQ=255) cAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAaaca < 1:180136/71‑1 (MQ=255) aaaaTAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGa > 1:2028173/1‑45 (MQ=255) aaTAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACgg > 1:482372/1‑71 (MQ=255) aaTAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACgg > 1:982836/1‑71 (MQ=255) tAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGata < 1:3138353/43‑3 (MQ=255) tAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGaaa < 1:1882950/43‑1 (MQ=255) tAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACTACGGCa < 1:522409/71‑1 (MQ=255) tAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCa < 1:2379224/71‑1 (MQ=255) cGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCgg < 1:3850866/71‑1 (MQ=255) gTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCgg < 1:254481/70‑1 (MQ=255) aCTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCgg > 1:2497653/1‑67 (MQ=255) aCTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCAc > 1:3554827/1‑70 (MQ=255) aCTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACt < 1:3715504/71‑1 (MQ=255) aCTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACt < 1:2538621/71‑1 (MQ=255) aCTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACt < 1:2012246/71‑1 (MQ=255) aCTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACt < 1:2455042/71‑1 (MQ=255) tGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTaa > 1:2402478/1‑71 (MQ=255) tGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTaa > 1:2669681/1‑71 (MQ=255) tGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTaa > 1:3275583/1‑71 (MQ=255) tGAATGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCaaa < 1:47424/47‑1 (MQ=255) aaTGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAAt < 1:2682182/70‑1 (MQ=255) aaTGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCaaaaa > 1:2414019/1‑47 (MQ=255) aaTGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCaaaaa > 1:302856/1‑47 (MQ=255) aaTGCTGACGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCaaaaa > 1:908235/1‑47 (MQ=255) aTGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAAt < 1:3349648/69‑1 (MQ=255) cTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAg > 1:3483050/1‑35 (MQ=255) cTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGagag > 1:3052717/1‑71 (MQ=255) cTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGagag > 1:11051/1‑71 (MQ=255) cTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGagag > 1:1982288/1‑71 (MQ=255) cTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGagag > 1:474472/1‑71 (MQ=255) cTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGagag > 1:2826090/1‑71 (MQ=255) cTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGagag > 1:2807250/1‑71 (MQ=255) aCGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCaa > 1:780758/1‑71 (MQ=255) aCGTCGGAAAACATGAGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGc < 1:2469169/69‑1 (MQ=255) cGGAAAACATGAGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGa > 1:3607616/1‑70 (MQ=255) ggAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGaca > 1:919524/1‑71 (MQ=255) ggAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGaca > 1:1125429/1‑71 (MQ=255) ggAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGaca > 1:118355/1‑71 (MQ=255) ggAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGaca < 1:1494379/71‑1 (MQ=255) ggAAAACATGAGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCCGCACTAATGAGAGCAACGaca < 1:3167326/71‑1 (MQ=255) aaaaCATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGAcaca < 1:1856709/71‑1 (MQ=255) aaaCATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACAt < 1:2837863/71‑1 (MQ=255) aaaCATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACAt < 1:1013905/71‑1 (MQ=255) cATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAgg > 1:1761749/1‑71 (MQ=255) cATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAgg > 1:3502915/1‑71 (MQ=255) cATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAgg > 1:3156470/1‑71 (MQ=255) aTGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGc < 1:2436363/71‑1 (MQ=255) gggAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGAcaca < 1:2974573/64‑1 (MQ=255) ggAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCaa < 1:1890338/70‑1 (MQ=255) ggAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCAAg > 1:409993/1‑71 (MQ=255) ggAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCAAg > 1:2039734/1‑71 (MQ=255) ggAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCAAg > 1:2714678/1‑71 (MQ=255) ggAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCAAg > 1:3543342/1‑71 (MQ=255) | TAGCAGGCAGAAAGTTGGCAAAAATAAAGTGACGTAACTGAATGCTGACGTCGGAAAACATGGGAAAAGCGGCGAAAAGCGCAAAAACAACGGCAACCAGCGGCACTAATGAGAGCAACGACACATAGGCAAG > W3110S.gb/3560289‑3560421 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |