| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,560,998 | A→G | 21.0% | F44S (TTT→TCT) | yihX ← | predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,560,998 | 0 | A | G | 21.0% | 75.1 / 17.4 | 38 | F44S (TTT→TCT) | yihX | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (19/11); new base G (2/6); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGCCATCTCATGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATACGCGTTAAATCGCTCCAGGCTC > W3110S.gb/3560930‑3561067 | gagCCATCTCATGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAAc > 1:3690143/1‑71 (MQ=255)gagCCATCTCATGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAAc > 1:2270938/1‑71 (MQ=255)gagCCATCTCATGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAAc > 1:2131865/1‑71 (MQ=255) tcATGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTcccc > 1:1746512/1‑71 (MQ=255) tcATGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTcccc > 1:343207/1‑71 (MQ=255) aTGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGcc < 1:2138419/64‑1 (MQ=255) aTGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTccc < 1:988202/68‑1 (MQ=255) acacAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAGACGCCTCCCCCatat > 1:397339/1‑71 (MQ=255) caGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAGACGCCTCCCCCATATGaa < 1:3528573/71‑1 (MQ=255) tGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAGACGCCTCCCCCATATGAAAACtctt < 1:2233075/69‑1 (MQ=255) tGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACtcttct > 1:578925/1‑71 (MQ=255) tGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCACCATATGAAAACtcttct > 1:3933745/1‑71 (MQ=255) cGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAGACGCCTCCCCCATATGAAAACTcttctt > 1:94290/1‑70 (MQ=255) aaCGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTc > 1:1155330/1‑47 (MQ=255) aCGCTTCGTCGCTAATTTCCTCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGc < 1:1856553/71‑1 (MQ=255) aCGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAGACGCCTCCCCCATATGAAAACTCTTCTTAAGc < 1:3638724/71‑1 (MQ=255) aCGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGc < 1:810590/71‑1 (MQ=255) aCGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGc < 1:777718/71‑1 (MQ=255) aCGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGc < 1:1341526/71‑1 (MQ=255) aCGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGc > 1:3496410/1‑71 (MQ=255) aCGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGc < 1:1884201/71‑1 (MQ=255) cgtcgCTAACTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCata > 1:3141158/1‑47 (MQ=255) gtcgCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCAAATGAAAACTCTTCTTAAGCGATGc > 1:3970517/1‑69 (MQ=255) gtcgCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCAAATGAAAACTCTTCTTAAGCGATGc > 1:1572347/1‑69 (MQ=255) tAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGaa < 1:3270131/45‑1 (MQ=255) aTTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGaaaa > 1:3860881/1‑45 (MQ=255) aTTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGaa < 1:455638/70‑1 (MQ=255) aTTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGaa < 1:176908/70‑1 (MQ=255) aTTTCCCCACGCTCATGCTAATGAAACGCCTCCCCCATATGaaaa > 1:1876443/1‑45 (MQ=255) tttCCCCACGCTCATGCTGATGAGACGCCTCCCCCATATGAAAACTCTTCTTAAGc < 1:3765549/56‑1 (MQ=255) tttCCCCACGCTCATGCTGATGAGACGCCTCCCCCATATGAAAACTCTTCTTAAGc < 1:1432496/56‑1 (MQ=255) tttCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGaa < 1:1274275/42‑1 (MQ=255) ttCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATAc > 1:1708018/1‑71 (MQ=255) ccccACGCGCATGCTGATGAGACGCCTCCCCCATATGAAAACTCTTCTTAAGc < 1:2956816/53‑1 (MQ=255) cATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATACGCGTTaaa > 1:2012823/1‑68 (MQ=255) gCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATACGCGTTAAATCGCTc > 1:504663/1‑71 (MQ=255) gaAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATACGCGTTAAATCGCTCCAGGCt > 1:1953598/1‑71 (MQ=255) gaAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATACGCGTTAAATCGCTCCAGGCt > 1:1703438/1‑71 (MQ=255) aaaCGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATACGCGTTAAATCGCTCCAGGCTc > 1:1049103/1‑71 (MQ=255) | GAGCCATCTCATGACACAGCGCCTCTGCGAACGCTTCGTCGCTAATTTCCCCACGCTCATGCTGATGAAACGCCTCCCCCATATGAAAACTCTTCTTAAGCGATGCCAGCGGAATACGCGTTAAATCGCTCCAGGCTC > W3110S.gb/3560930‑3561067 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |