Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,566,219 A→G 40.0% intergenic (+53/‑61) yihS → / → yihR predicted glucosamine isomerase/predicted aldose‑1‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,566,2190AG40.0% 4.0 / 13.6 15intergenic (+53/‑61)yihS/yihRpredicted glucosamine isomerase/predicted aldose‑1‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (7/2);  new base G (3/3);  total (10/5)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGATCTGAAAATAGGGTGCGGGTTTTCCCCTCTCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTCCGGTGCAGTTTTTGGGTTTGACATCAGCCTCACGGGAGGCTGTATGTCGT  >  W3110S.gb/3566167‑3566286
                                                    |                                                                   
cTGATCTGAAAATAGGGTGCGGGTTTTCCCCTCTCCCTAACCCTCTCCCCAAAgg                                                                   >  1:1262621/1‑55 (MQ=255)
                               tctcCCTAACCCTC‑CCCCAAAGGGGCGAGGGGACCGTCCGGTGCAGTTTTTGGGTTTGACATCAGCCTc                     <  1:3019410/69‑1 (MQ=255)
                                 tcCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTCCGGTGc                                              >  1:507825/1‑43 (MQ=255)
                                 tcCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTCCGGTGCAGTTTTTGGGTTTGACATCAGCCTc                     <  1:1882995/68‑1 (MQ=255)
                                     tAACCCTCTCCCCAAGGGGGCGAGGGGACCGTCCGGTGCAGTTTTTGGGTTTGa                               <  1:2507782/54‑1 (MQ=255)
                                     tAACCCTCTCCCCAAGGGGGCGAGGGGACCGTCCGGTGCAGTTTTTGGGTTTGa                               <  1:25256/54‑1 (MQ=255)
                                     tAACCCTCTCCCCAAGGGGGCGAGGGGACCGTCCGGTGCAGTTTTTGGGTTTGa                               <  1:3710192/54‑1 (MQ=255)
                                      aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTCCGGTGCAGTTTTTGGGTTTGACATCAGCCTCACGGGAgg             >  1:2408663/1‑71 (MQ=255)
                                            ctcCCCAAGGGGGCGAGGGGACCGTCCGGTGCAGTTTTTGGGTTTGACATCAGCCTCACGGGAGGCTGTAt       >  1:2878209/1‑71 (MQ=255)
                                               cccAAGGGGGCGAGGGGACCGTCCGGTGCAGTTTTTGGGTTTGACATCAGCCTCACGGGAGGCTGTa        >  1:3392578/1‑67 (MQ=255)
                                               cccAAAGGGGCGAGGGGACCGTCCGGTGCAGTTTTTGGGTTTGACATCAGCCTCACGGGAGGCTGTAt       >  1:3413427/1‑68 (MQ=255)
                                               cccAAAGGGGCGAGGGGACCGTCCGGTGCAGTTTTTGGGTTTGAAATCAGCCTCACGGGAGGCTGTAt       >  1:1736394/1‑68 (MQ=255)
                                                 cAAGGGGGCGAGGGGACCGTCCGGTGCAGTTTTTGGGTTTGACATCAGCCTCACGGGAGGCTGTATGTCGt  >  1:2181853/1‑71 (MQ=255)
                                                 cAAAGGGGCGAGGGGACCGTCCGGTGCAGTTTTTGGGTTTGa                               >  1:1996402/1‑42 (MQ=255)
                                                 cAAAGGGGCGAGGGGACCGTCCGGTGCAGTTTTTGGGTTTGACATCAGCCTCACGGGAGGCTGTATGTCGt  >  1:8536/1‑71 (MQ=255)
                                                    |                                                                   
CTGATCTGAAAATAGGGTGCGGGTTTTCCCCTCTCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTCCGGTGCAGTTTTTGGGTTTGACATCAGCCTCACGGGAGGCTGTATGTCGT  >  W3110S.gb/3566167‑3566286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑