| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,591,039 | G→A | 25.0% | intergenic (+28/+13) | yihG → / ← yihF | predicted endonuclease/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,591,039 | 0 | G | A | 25.0% | 48.2 / 14.4 | 28 | intergenic (+28/+13) | yihG/yihF | predicted endonuclease/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (13/8); new base A (3/4); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTTCTGACGTCTCTCATGTCATCACAGCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAATTCTTCTTCGCTCATCTC > W3110S.gb/3590969‑3591102 | gCTTCTGACGTCTCTCATGTCATCACAGCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAgg > 1:3239149/1‑71 (MQ=255) gACGTCTCTCATGTCATCACAGCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCgagt > 1:1143974/1‑70 (MQ=255) aTGTCATCACAGCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCGAGTTAGTAtttt < 1:2539557/69‑1 (MQ=255) aTGTCATCACAGCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTAtttt < 1:93672/69‑1 (MQ=255) aTGGCATCACAGCGTCAGAATAAGTAAGATTTACTCGCCTCTGAATGAGCAGAGACGAGTGAGTATTTTa < 1:3865248/70‑1 (MQ=255) tcatcaCAGCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATg > 1:1565027/1‑70 (MQ=255) acaGCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACaa < 1:921282/70‑1 (MQ=255) gCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACg < 1:1627310/71‑1 (MQ=255) gCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACg < 1:594165/71‑1 (MQ=255) aGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCgg > 1:1517847/1‑71 (MQ=255) aGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGACGAGTGAGTATTTTAATGAACAAAACGTCCgg > 1:1424972/1‑71 (MQ=255) aGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGACGAGTGAGTATTTTAATGAACAAAACGTCCgg > 1:846133/1‑71 (MQ=255) aTAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGACGAGTGAGTATTTTAATGAACAAAACGTCCGGCAc < 1:1837964/71‑1 (MQ=255) aTAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGACGAGTGAGTATTTTAATGAACAAAACGTCCGGCAc < 1:3663870/71‑1 (MQ=255) agtaagtTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCAc > 1:3710482/1‑68 (MQ=255) agtTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACa > 1:3990911/1‑70 (MQ=255) tttACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACgaga > 1:146318/1‑65 (MQ=255) tttACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACgaga > 1:1907774/1‑65 (MQ=255) cTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAAttc < 1:1784704/70‑1 (MQ=255) tctGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAATTCTTCTTCg > 1:2620687/1‑71 (MQ=255) tctGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAATTCTTCTTCg > 1:602400/1‑71 (MQ=255) tGAATGAGCAGAGGCGAGTGAGTATTTTAATGAAc > 1:1720386/1‑35 (MQ=255) tGAATGAGCAGAGGCGAGTGAGTATTTTAATGAAc > 1:2395685/1‑35 (MQ=255) tGAATGAGCAGAGGCGAGTGAGTATTTTAATGAAc > 1:1438776/1‑35 (MQ=255) tGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACa < 1:780786/36‑1 (MQ=255) tGAGCAGAGACGAGTGAGTATTTTAATGAACAAAACGTCCg > 1:3942244/1‑41 (MQ=255) aGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAATTCTTCTTCGCTCAtctc > 1:3491425/1‑71 (MQ=255) gCAGAGACGAGTGAGTATTTTAATGAACAAAACGTCCGTCACGAGACATAAATTCTTCTTCGCTCAtctc < 1:3618243/70‑1 (MQ=255) cAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCAc < 1:66150/41‑1 (MQ=255) | GCTTCTGACGTCTCTCATGTCATCACAGCGTCAGAATAAGTAAGTTTTACTCGCCTCTGAATGAGCAGAGGCGAGTGAGTATTTTAATGAACAAAACGTCCGGCACGAGACATAAATTCTTCTTCGCTCATCTC > W3110S.gb/3590969‑3591102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |