| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,602,757 | T→A | 31.3% | L260F (TTA→TTT) | trkH ← | potassium transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,602,757 | 0 | T | A | 31.3% | 82.4 / 57.3 | 64 | L260F (TTA→TTT) | trkH | potassium transporter |
| Reads supporting (aligned to +/- strand): ref base T (21/23); new base A (8/12); total (29/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.79e-01 | |||||||||||
GCGTAAACTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGTTAATAG > W3110S.gb/3602687‑3602827 | gCGTAAACTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACtt < 1:1094108/71‑1 (MQ=255) cGTAAACTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACtt < 1:311678/70‑1 (MQ=255) tAAACTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACtt < 1:3285115/68‑1 (MQ=255) tAAACTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACtt < 1:1161651/68‑1 (MQ=255) aaCTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACa > 1:1073069/1‑70 (MQ=255) aaCTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAg > 1:415674/1‑71 (MQ=255) tcTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACa < 1:2829112/68‑1 (MQ=255) tACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTaa > 1:320427/1‑63 (MQ=255) tACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTaa > 1:3052586/1‑63 (MQ=255) cGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACa > 1:2746753/1‑63 (MQ=255) cGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACa > 1:2332657/1‑63 (MQ=255) cGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAg > 1:980109/1‑71 (MQ=255) cGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAg > 1:257588/1‑71 (MQ=255) cGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAg > 1:2031280/1‑71 (MQ=255) ccGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGtt < 1:3261516/71‑2 (MQ=255) ccGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:200269/71‑1 (MQ=255) ccGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:1348692/71‑1 (MQ=255) cGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:1771997/70‑1 (MQ=255) cGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:2537062/70‑1 (MQ=255) gATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:1126035/69‑1 (MQ=255) gATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:4022518/69‑1 (MQ=255) aaaCATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGAc > 1:2730956/1‑71 (MQ=255) aTGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTa < 1:811044/71‑1 (MQ=255) aaTTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAg > 1:285543/1‑66 (MQ=255) aaTTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAg > 1:3801609/1‑66 (MQ=255) aaTTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACa < 1:2813700/71‑1 (MQ=255) aaTTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACa < 1:520206/71‑1 (MQ=255) tCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTaaa > 1:3089910/1‑41 (MQ=255) tCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTaa > 1:1936238/1‑41 (MQ=255) tCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTaa > 1:1095420/1‑41 (MQ=255) tCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTa > 1:2024202/1‑40 (MQ=255) ccGGATCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCg > 1:701599/1‑71 (MQ=255) gATCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGgag < 1:1106959/71‑1 (MQ=255) aTCGCGCCAATAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGgag < 1:2888/70‑1 (MQ=255) tCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGAt < 1:795532/71‑1 (MQ=255) ccAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:3392117/42‑1 (MQ=255) ccAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:2343858/42‑1 (MQ=255) ccAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:1877418/42‑1 (MQ=255) ccAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:3063942/42‑1 (MQ=255) aaTAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCa > 1:2871351/1‑42 (MQ=255) aaTAAACCTTCAGACTACGCCCACTAAACAGTGGAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag > 1:3499311/1‑70 (MQ=255) aaTAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag > 1:3625325/1‑70 (MQ=255) aaTAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag > 1:419306/1‑70 (MQ=255) aaTAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcaccag > 1:3395442/1‑70 (MQ=255) aTAAACCTTCAGACTACGCCCACTTAACAGTGaaaa < 1:3790531/36‑1 (MQ=255) tAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTg < 1:2578968/38‑1 (MQ=255) tAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGaa < 1:2478114/71‑1 (MQ=255) tAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGaa < 1:2836058/71‑1 (MQ=255) tAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGaa < 1:364655/71‑1 (MQ=255) tAAACCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGaa < 1:3580994/71‑1 (MQ=255) aaCCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAgg > 1:2306806/1‑67 (MQ=255) aaCCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAgg > 1:2500473/1‑67 (MQ=255) aaCCTTCAGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAgg > 1:2534000/1‑67 (MQ=255) ttCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGaa < 1:340653/65‑1 (MQ=255) aGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAAt < 1:3570258/71‑1 (MQ=255) aGACTACGCCCACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAAt < 1:3659422/71‑1 (MQ=255) cTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGAt < 1:164969/71‑1 (MQ=255) ccACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGtt < 1:1053760/71‑1 (MQ=255) ccACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGtt < 1:3222712/71‑1 (MQ=255) ccACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag < 1:2820260/50‑1 (MQ=255) ccACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag < 1:4003792/50‑1 (MQ=255) ccACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag < 1:2371622/50‑1 (MQ=255) ccACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag < 1:2197538/50‑1 (MQ=255) ccACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagcag < 1:1795814/50‑1 (MQ=255) cACTAAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagca > 1:2541306/1‑48 (MQ=255) cTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGa > 1:1256311/1‑61 (MQ=255) cTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGa > 1:2505972/1‑61 (MQ=255) cTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGTTaa > 1:827220/1‑70 (MQ=255) cTTAACAGTGAAAAGTCCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGa > 1:2724716/1‑61 (MQ=255) tAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagca > 1:3368651/1‑44 (MQ=255) tAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagca > 1:3060631/1‑44 (MQ=255) tAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATcagca > 1:2124266/1‑44 (MQ=255) tAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGTTaa > 1:1734175/1‑68 (MQ=255) tAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGTTAATAg > 1:1225101/1‑71 (MQ=255) | GCGTAAACTGTACGCCGATAAACATGCGAAATTCCGGATCGCGCCAATAAACCTTCAGACTACGCCCACTTAACAGTGAAAAGTGCAGACCGTAGTTACAGCCGGAGATCAGCAGGAAGATAGCAATGATAGTGTTAATAG > W3110S.gb/3602687‑3602827 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |