| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,608,041 | C→T | 23.3% | L45L (CTG→TTG) | fadA → | 3‑ketoacyl‑CoA thiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,608,041 | 0 | C | T | 23.3% | 57.6 / 12.3 | 30 | L45L (CTG→TTG) | fadA | 3‑ketoacyl‑CoA thiolase |
| Reads supporting (aligned to +/- strand): ref base C (9/14); new base T (4/3); total (13/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAACGTGCGTGCAGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTGCAGCAGACGCTGGAGCAGGGTTTT > W3110S.gb/3607976‑3608106 | gTAACGTGCGTGCAGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGTTGGa < 1:2160912/70‑1 (MQ=255) cAGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTc > 1:3328773/1‑71 (MQ=255) aagaTCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCgtcggc < 1:3534803/64‑1 (MQ=255) aagaTCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCggcggc < 1:3676/64‑1 (MQ=255) aagaTCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCggcggc < 1:3483613/64‑1 (MQ=255) aagaTCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTcga > 1:737282/1‑70 (MQ=255) aagaTCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTcga > 1:170776/1‑70 (MQ=255) gaTCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTcgacga < 1:1880121/71‑1 (MQ=255) aTCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTcgac < 1:2409307/68‑1 (MQ=255) ctctcCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTcgacga > 1:69941/1‑68 (MQ=255) ctctcCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGTTGGAAGCGGCGGCCCTcgacga > 1:2823385/1‑68 (MQ=255) ctctcCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGata > 1:3548611/1‑70 (MQ=255) tcCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGat < 1:1240702/66‑1 (MQ=255) gCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTg < 1:2487924/71‑1 (MQ=255) tCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATAtt < 1:2974019/64‑1 (MQ=255) tCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATAtt < 1:1223665/64‑1 (MQ=255) tCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATAtt < 1:563950/64‑1 (MQ=255) tCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATAtt < 1:330844/64‑1 (MQ=255) aTTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATAttt < 1:2643189/63‑1 (MQ=255) tAATGCTTAGCCTGCTGGCGCGTAACCCGGCGTTGGAAg > 1:522379/1‑39 (MQ=37) tAATGCGTAGCCTGCTGGCGCGTAACCCGGCGTTGGAAg > 1:1399357/1‑39 (MQ=255) tAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTtgt > 1:364680/1‑71 (MQ=255) tAAAGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGCCGATATTTACTGGGGTtgt > 1:359700/1‑71 (MQ=255) ctgGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTGCAGCAGACGCt < 1:3540529/71‑1 (MQ=255) ctgGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTGCAGCAGACGCt < 1:2759850/71‑1 (MQ=255) gcgcGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTa > 1:4020199/1‑44 (MQ=255) gTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTGCAGCAGACGCTGGAGCAg > 1:2494911/1‑71 (MQ=255) cGGCGTTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTGCAGCAGACGCTGGAGCAGGGtttt < 1:3545297/71‑1 (MQ=255) cGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTgca > 1:2323055/1‑49 (MQ=255) cGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTGCAGCAGACGCTGGAGCAGGGtttt < 1:1775667/71‑1 (MQ=255) | GTAACGTGCGTGCAGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTTACTGGGGTTGTGTGCAGCAGACGCTGGAGCAGGGTTTT > W3110S.gb/3607976‑3608106 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |