| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 286,496 | T→C | 27.8% | F162L (TTT→CTT) | yagH → | predicted xylosidase/arabinosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 286,496 | 0 | T | C | 27.8% | 52.0 / 23.8 | 36 | F162L (TTT→CTT) | yagH | predicted xylosidase/arabinosidase |
| Reads supporting (aligned to +/- strand): ref base T (14/12); new base C (8/2); total (22/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTA > W3110S.gb/286434‑286563 | tCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGCTTGa > 1:3138544/1‑67 (MQ=255) tGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCAc < 1:1888594/71‑1 (MQ=255) gggCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGct < 1:1758623/71‑1 (MQ=255) gggCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGct < 1:2378023/71‑1 (MQ=255) gggCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGCTTGACCCGCAGACCGGCACGct < 1:2476811/71‑1 (MQ=255) cgcgCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCa > 1:3352818/1‑55 (MQ=255) gcgcCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGCTTGACCCGCAGACCGGCACGCTCTCGc > 1:1439920/1‑71 (MQ=255) gcgcCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGCTTGACCCGCAGACCGGCACGCTCTCGc > 1:428298/1‑71 (MQ=255) acaGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACTCTCTCGccc > 1:3130909/1‑65 (MQ=255) acaGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAgcg > 1:1456238/1‑70 (MQ=255) acaGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAgcg > 1:571090/1‑70 (MQ=255) caGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGAc > 1:1670283/1‑40 (MQ=255) caGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGAc > 1:2241637/1‑40 (MQ=255) aCCCGCACAACACCATCGTGTTACAGGCGCTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAAc > 1:744000/1‑70 (MQ=255) ccGCACAACACCATCGTGTTACAGGCGCTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCt < 1:3085518/71‑1 (MQ=255) cGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTg > 1:1866210/1‑71 (MQ=255) cGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTg > 1:1503835/1‑71 (MQ=255) cGCACAACACCATCGTGTTACAGGCGCTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTg > 1:566660/1‑71 (MQ=255) acaacaCCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGttt < 1:450720/71‑1 (MQ=255) acaacaCCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGttt < 1:2462703/71‑1 (MQ=255) acaacaCCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGttt < 1:1318696/71‑1 (MQ=255) caacaCCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTa < 1:3778290/71‑1 (MQ=255) acCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCgg < 1:727212/71‑1 (MQ=255) cATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCa < 1:3095201/71‑1 (MQ=255) gtgtTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTa > 1:984581/1‑61 (MQ=255) gtgtTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTa > 1:1539907/1‑61 (MQ=255) gtgtTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCACGAGCGCAAAACGCTGTTTa > 1:189907/1‑61 (MQ=255) tgtTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCAcgccg < 1:151630/71‑1 (MQ=255) tgtTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCAcgccg < 1:1180781/71‑1 (MQ=255) tgtTACAGGCGTTTGACCCGCAGACCGGCACCCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCAcgccg < 1:1113598/71‑1 (MQ=255) gtTACAGGCGCTTGACCCGCAGACCGGCACGCTCTCg > 1:3446387/1‑37 (MQ=255) gtTACAGGCGCTTGACCCGCAGACCGGCACGCTCTCGcc > 1:3177159/1‑39 (MQ=255) gtTACAGGCGCTTGACCCGCAGACCGGCACGCTCTCGcc > 1:626370/1‑39 (MQ=255) ttACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGct > 1:3546187/1‑71 (MQ=255) ttACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGct > 1:271847/1‑71 (MQ=255) gCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTa > 1:524902/1‑71 (MQ=255) | TCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTA > W3110S.gb/286434‑286563 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |