| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,633,778 | T→C | 22.2% | C97C (TGT→TGC) | yigG → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,633,778 | 0 | T | C | 22.2% | 156.2 / 46.0 | 81 | C97C (TGT→TGC) | yigG | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (24/39); new base C (9/9); total (33/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01 | |||||||||||
ATGTTTTTGGTACATTAGCAGTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAATGCTTGC > W3110S.gb/3633711‑3633843 | aTGTTTTTGGTACATTAGCAGTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGtt > 1:1423617/1‑69 (MQ=255) aGCAGTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCgatga > 1:3098846/1‑71 (MQ=255) aGCAGTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCgatga > 1:3103576/1‑71 (MQ=255) aGCAGTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCgatga > 1:151596/1‑71 (MQ=255) tatataTCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGc < 1:3025368/53‑1 (MQ=255) tatataTCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGc < 1:3014295/53‑1 (MQ=255) tatataTCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGc < 1:2112234/53‑1 (MQ=255) tatataTCATCTCTATCATCACAATGATACCCGAAGATTTTGCCTGTTCAGGc < 1:3213101/53‑1 (MQ=255) tatatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCaa > 1:3637448/1‑53 (MQ=255) tatatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAAt > 1:3755146/1‑54 (MQ=255) tatatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGAttttttt > 1:4052915/1‑71 (MQ=255) tatatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGAttttttt > 1:1525374/1‑71 (MQ=255) tatatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGAttttt > 1:3020365/1‑69 (MQ=255) tatatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGCTCAGGCAATGGTTCGATGAttttttt > 1:3699876/1‑71 (MQ=255) tatatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGCTCAGGCAATGGTTCGATGAtttttt > 1:2865025/1‑70 (MQ=255) tatatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGCTCAGGCAATGGTTCGATGAtttttt > 1:1989108/1‑70 (MQ=255) tatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGa > 1:29354/1‑71 (MQ=255) tatCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTAGATGATTTTTTTGa > 1:2381784/1‑71 (MQ=255) atcatcTCTATCATCACAATGATAGCCGAAGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGAt > 1:2155149/1‑71 (MQ=255) tcatcTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGAtttt < 1:1226611/64‑1 (MQ=255) tcatcTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGAtt > 1:1747172/1‑71 (MQ=255) tcatcTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGAt < 1:710501/70‑1 (MQ=255) tcatcTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGAt < 1:4030245/70‑1 (MQ=255) tctATCGTCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTa > 1:525853/1‑71 (MQ=255) tctATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTa > 1:2764340/1‑71 (MQ=255) tctATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTa > 1:4029064/1‑71 (MQ=255) tATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGt < 1:3946982/67‑1 (MQ=255) tATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGt < 1:4006902/67‑1 (MQ=255) atcatcACAATGATAGCCGAAGATTTTGCCTGCTCAGGc < 1:639849/39‑1 (MQ=255) atcatcACAATGATAGCCGAAGATTTTGCCTGCTCAGGc < 1:209978/39‑1 (MQ=255) tcatcaCAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTAtttt < 1:2240992/71‑1 (MQ=255) tcatcaCAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTAt < 1:2724394/68‑1 (MQ=255) atcaCAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTc > 1:2128647/1‑71 (MQ=255) atcaCAATGATAGCCGAAGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTc > 1:3797784/1‑71 (MQ=255) cacaATGATAGCCGAAGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCa > 1:2954946/1‑71 (MQ=255) tGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACtt < 1:2085505/69‑1 (MQ=255) tGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACtt < 1:963778/69‑1 (MQ=255) aTAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAg < 1:2892080/71‑1 (MQ=255) aTAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAg < 1:1047330/71‑1 (MQ=255) aTAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAg > 1:413138/1‑71 (MQ=255) aTAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAg < 1:991912/71‑1 (MQ=255) aTAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAg < 1:1057580/71‑1 (MQ=255) aTAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAg < 1:2874476/71‑1 (MQ=255) aTAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAg < 1:1511967/71‑1 (MQ=255) aTAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAg > 1:1385610/1‑71 (MQ=255) aTAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAg < 1:2887422/71‑1 (MQ=255) tAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGAttttttt < 1:310489/46‑1 (MQ=255) tAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGAttttttt < 1:1693879/46‑1 (MQ=255) tAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAg < 1:1463582/70‑1 (MQ=255) tAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAg < 1:2688250/70‑1 (MQ=255) tAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAg < 1:254397/70‑1 (MQ=255) tAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGt < 1:3489967/71‑1 (MQ=255) tAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCAACTTTCAg < 1:1402131/70‑1 (MQ=255) tAGCCGAAGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTg < 1:23804/52‑1 (MQ=255) aGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTa > 1:3945624/1‑71 (MQ=255) aGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTa > 1:3945622/1‑71 (MQ=255) gCCGAAGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTAt < 1:138946/71‑1 (MQ=255) gAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCt < 1:3844669/71‑1 (MQ=255) gAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCt < 1:3349739/71‑1 (MQ=255) gAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCt < 1:3222227/71‑1 (MQ=255) gAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCt < 1:2723305/71‑1 (MQ=255) gAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCt < 1:806658/71‑1 (MQ=255) gAAGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCt < 1:2593936/71‑1 (MQ=255) aaGATTTTGCCTGTTCAGGCAATGGTTCGATGAttttttt < 1:2315714/40‑1 (MQ=255) aaGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTa < 1:980693/49‑1 (MQ=255) aaGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCt < 1:1905290/70‑1 (MQ=255) aaGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCt < 1:1305168/70‑1 (MQ=255) aaGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCt < 1:2762772/70‑1 (MQ=255) aaGATTTTGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCt < 1:2859377/70‑1 (MQ=255) gATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAAt < 1:565976/71‑1 (MQ=255) gATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAAt < 1:2662036/71‑1 (MQ=255) tttGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAATGCt > 1:2281675/1‑71 (MQ=255) tttGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAATGCt > 1:4021281/1‑71 (MQ=255) tttGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAATGCt > 1:3378326/1‑71 (MQ=255) tttGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAATGCt > 1:494673/1‑71 (MQ=255) tttGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCAc > 1:3810856/1‑53 (MQ=255) tttGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCAc > 1:2341960/1‑53 (MQ=255) tttGCCTGCTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCAc > 1:3201872/1‑53 (MQ=255) ttGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAATGCtt > 1:2884495/1‑71 (MQ=255) ccTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAATGCTTGc < 1:3792151/70‑1 (MQ=255) gTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTAttt < 1:3188169/40‑1 (MQ=255) gTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAg < 1:1860191/52‑1 (MQ=255) | ATGTTTTTGGTACATTAGCAGTATATATCATCTCTATCATCACAATGATAGCCGAAGATTTTGCCTGTTCAGGCAATGGTTCGATGATTTTTTTGATTTGTTATTTTTCCACTTTCAGTATGCTAATGCTTGC > W3110S.gb/3633711‑3633843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |