| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,671,661 | G→A | 29.1% | R204Q (CGG→CAG) | rhlB → | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,671,661 | 0 | G | A | 29.1% | 84.4 / 40.0 | 55 | R204Q (CGG→CAG) | rhlB | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (25/14); new base A (9/7); total (34/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GTTCCGCCGTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGAACAGAAAA > W3110S.gb/3671596‑3671729 | gTTCCGCCGTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTAc > 1:1458007/1‑71 (MQ=255) ttCCGCCGTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTAc < 1:3969709/70‑1 (MQ=255) ccgccgTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGt > 1:1544529/1‑70 (MQ=255) cgccgTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTg < 1:631305/70‑1 (MQ=255) tATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTg < 1:2254440/70‑1 (MQ=255) tATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTg < 1:2929413/70‑1 (MQ=255) ccgccTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTa > 1:808671/1‑57 (MQ=255) ccgccTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTa > 1:1613832/1‑57 (MQ=255) ccgccTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTa > 1:2754994/1‑57 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCa < 1:3618124/70‑1 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCa < 1:1441179/70‑1 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAg > 1:4085141/1‑68 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAg > 1:549788/1‑68 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAg > 1:3632328/1‑68 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAg > 1:3288307/1‑68 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAg > 1:1739300/1‑68 (MQ=255) cAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCa < 1:1505287/70‑1 (MQ=255) aCCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTg < 1:2108654/51‑1 (MQ=255) aCCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTg < 1:1907075/51‑1 (MQ=255) ccAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGaa > 1:1658277/1‑52 (MQ=255) ccAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGaa > 1:3556278/1‑52 (MQ=255) cAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGaa > 1:972566/1‑51 (MQ=255) gcCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAAt < 1:110812/71‑1 (MQ=255) gcCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAAt < 1:1778153/71‑1 (MQ=255) tCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACaa > 1:25770/1‑67 (MQ=255) tCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACaa > 1:1694087/1‑67 (MQ=255) tCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACaa > 1:3675772/1‑67 (MQ=255) aaCATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCa > 1:337070/1‑56 (MQ=255) aaCATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCa > 1:869155/1‑56 (MQ=255) tGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGaa > 1:503820/1‑38 (MQ=255) tGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAata > 1:1523245/1‑70 (MQ=255) cTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAatatat > 1:1332914/1‑71 (MQ=255) ttCTCCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTg < 1:1986258/70‑1 (MQ=255) ctcCGCGACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGATCa > 1:521316/1‑53 (MQ=255) ctcCGCCACGCTTTCGTATCGGGTACGTGAACTGGCGTTCGAGCAGATGAACaa > 1:1296119/1‑54 (MQ=255) ctcCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGa < 1:889354/69‑1 (MQ=255) ctcCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAg > 1:3868121/1‑71 (MQ=255) ctcCGCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAg > 1:1620213/1‑71 (MQ=255) cGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGaa > 1:359536/1‑67 (MQ=255) gcgaCGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTgg < 1:2140536/67‑1 (MQ=255) gCCACGCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGa < 1:166163/71‑1 (MQ=255) cACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACa > 1:3656107/1‑47 (MQ=255) cACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACa > 1:998929/1‑47 (MQ=255) aCGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAAcc > 1:105675/1‑71 (MQ=255) gCTTTCGTACCAGGTACGTGAACTGGCGTTCGAGCAGATGAACAAt < 1:2427545/46‑1 (MQ=255) ttCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGa < 1:520690/57‑1 (MQ=255) cGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAg > 1:3800381/1‑57 (MQ=255) tACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGAACAGaa < 1:423268/71‑1 (MQ=255) tACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGAACAGaa < 1:142412/71‑1 (MQ=255) tACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGAACAGaa < 1:1471425/71‑1 (MQ=255) tACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGAACAGaa < 1:2977498/71‑1 (MQ=255) ccGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGAACAGaaaa > 1:1222672/1‑71 (MQ=255) ccGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGAACAGaaaa > 1:3736708/1‑71 (MQ=255) ccGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGAACAGaaaa > 1:2941830/1‑71 (MQ=255) ccGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGAACAGaaaa > 1:2527463/1‑71 (MQ=255) | GTTCCGCCGTATGCCGCCTGCAAACCAGCGCCTCAACATGCTGTTCTCCGCCACGCTTTCGTACCGGGTACGTGAACTGGCGTTCGAGCAGATGAACAATGCCGAATATATTGAAGTGGAACCGGAACAGAAAA > W3110S.gb/3671596‑3671729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |