| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,701,075 | T→C | 35.3% | L107L (TTA→TTG) | rbsC ← | D‑ribose transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,701,075 | 0 | T | C | 35.3% | 46.9 / 51.1 | 51 | L107L (TTA→TTG) | rbsC | D‑ribose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (19/14); new base C (16/2); total (35/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CGTAGCGATAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTGCGC > W3110S.gb/3701006‑3701145 | cGTAGCGATAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCt > 1:687500/1‑70 (MQ=255) gCGATAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAAcgc < 1:3128066/71‑1 (MQ=255) gCGATAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAAcgc < 1:3465346/71‑1 (MQ=255) gCGATAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAAcgc > 1:2825570/1‑71 (MQ=255) gCGATAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAAcgc > 1:1814178/1‑71 (MQ=255) aTAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCt < 1:2885706/63‑1 (MQ=255) tAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCt < 1:2385899/62‑1 (MQ=255) tAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCga > 1:43556/1‑69 (MQ=255) aaaCGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGagag > 1:1357116/1‑71 (MQ=255) aaCGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGagc < 1:3930388/71‑1 (MQ=255) aaCGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGagag > 1:2231636/1‑70 (MQ=255) aaCGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGagag > 1:3811515/1‑70 (MQ=255) cGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGagcag > 1:642661/1‑71 (MQ=255) gCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGagcagc < 1:1328511/71‑1 (MQ=255) gACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGa > 1:976781/1‑71 (MQ=255) gACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGAGCAGCAGCGa > 1:3111856/1‑71 (MQ=255) gACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGAGCAGCAGCGa > 1:2154978/1‑71 (MQ=255) gACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGAGCAGCAGCGa > 1:115042/1‑71 (MQ=255) gACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGAGCAGCAGCGa > 1:2641868/1‑71 (MQ=255) gcgACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAg > 1:1444322/1‑71 (MQ=255) gcgACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAg > 1:2807311/1‑71 (MQ=255) tCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGagca > 1:1796780/1‑53 (MQ=255) tCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGagca > 1:554739/1‑53 (MQ=255) gCTACAATCACCCCGGTTACCGCACCAATTGCGTCACCTAACGCGAGAGCAGCAGCGACAGCCACCAgcgc < 1:2829168/71‑1 (MQ=255) gCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAgcg < 1:4079057/70‑1 (MQ=255) gCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAgcg < 1:1286897/70‑1 (MQ=255) gCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGAGCAGCAGCGACAGCCACCAgcg < 1:58327/70‑1 (MQ=255) cAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCAtt > 1:335258/1‑70 (MQ=255) cAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCAtt < 1:1046247/70‑1 (MQ=255) cACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACtt > 1:2880640/1‑71 (MQ=255) cACCCCGGTTACCGCACCAATTGCGGCACCCAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACtt > 1:139887/1‑71 (MQ=255) aCCCCGGTTACCGCACCAATTGCGGCACCTAACGCga > 1:3213189/1‑37 (MQ=255) aCCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTc < 1:1449296/71‑1 (MQ=255) ccccGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCa < 1:2588433/71‑1 (MQ=255) cccGGTTACCGCACCAATTGCGGCACCCAACGCTAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCaa < 1:1454407/71‑1 (MQ=255) tACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGGGACAGCCACCAGCGCATTGACTTCAATGCcga > 1:243557/1‑71 (MQ=255) tACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCcga > 1:598971/1‑71 (MQ=255) tACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCcga > 1:1891827/1‑71 (MQ=255) aCCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATa < 1:583745/70‑1 (MQ=255) aCCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAg < 1:356774/71‑1 (MQ=255) aCCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAg < 1:898984/71‑1 (MQ=255) aaTTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCcgacga > 1:3772184/1‑65 (MQ=255) aaTTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCcgacga > 1:538255/1‑65 (MQ=255) aaTTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCcgacga > 1:822540/1‑65 (MQ=255) aaTTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCcgacga > 1:1991391/1‑65 (MQ=255) aaTTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCcgacga > 1:2108367/1‑65 (MQ=255) aaTTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAg > 1:3967724/1‑68 (MQ=255) aaTTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGa > 1:1009612/1‑69 (MQ=255) aaTTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGa > 1:3165222/1‑69 (MQ=255) aaTTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGa > 1:1290747/1‑69 (MQ=255) aaTTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATg < 1:992095/71‑1 (MQ=255) aaTTGCGGCACCCAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATg > 1:2189946/1‑71 (MQ=255) aTTGCGGCACCTAACGCGAGAGCAGAAGCGACAGCCACCAGCGCATTGACTTCAATGCcgacga > 1:1233660/1‑64 (MQ=255) gCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATgcag > 1:3977004/1‑70 (MQ=255) tAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCaa > 1:2498934/1‑44 (MQ=255) tAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCaa > 1:176173/1‑44 (MQ=255) tAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCcgac < 1:1741254/51‑1 (MQ=255) tAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCcgac < 1:1750102/51‑1 (MQ=255) tAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTgcgc > 1:1686291/1‑71 (MQ=255) tAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTgcgc > 1:3374947/1‑71 (MQ=255) | CGTAGCGATAAACGCCTGGACGCGACCTTTCGCTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTGCGC > W3110S.gb/3701006‑3701145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |