| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,712,033 | G→A | 31.8% | M328I (ATG→ATA) | gidA → | glucose‑inhibited cell‑division protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,712,033 | 0 | G | A | 31.8% | 87.1 / 56.4 | 66 | M328I (ATG→ATA) | gidA | glucose‑inhibited cell‑division protein |
| Reads supporting (aligned to +/- strand): ref base G (24/21); new base A (10/11); total (34/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01 | |||||||||||
ATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCT > W3110S.gb/3711963‑3712099 | aTGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATg < 1:1179788/71‑1 (MQ=255) tGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGc < 1:652162/71‑1 (MQ=255) tGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGc < 1:2943179/71‑1 (MQ=255) aTTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAggg < 1:3402535/71‑1 (MQ=255) aTTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAggg < 1:554490/71‑1 (MQ=255) ttATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGa > 1:3071194/1‑71 (MQ=255) tATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGa > 1:3216946/1‑70 (MQ=255) tATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGCCCGCTCTATGCAgggg > 1:266452/1‑69 (MQ=255) cGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGaa < 1:1580786/71‑1 (MQ=255) cGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGaa < 1:2534068/71‑1 (MQ=255) cGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGa > 1:1307359/1‑70 (MQ=255) ctcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGaa < 1:788332/61‑1 (MQ=255) tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAAcgcg > 1:1176784/1‑66 (MQ=255) tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAAcgcg > 1:552094/1‑66 (MQ=255) tcCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAAcgcg > 1:2082313/1‑66 (MQ=255) caccaGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATc > 1:1283303/1‑70 (MQ=255) caccaGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATg < 1:292103/55‑1 (MQ=255) caccaGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGAt < 1:920351/69‑1 (MQ=255) caccaGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGCAAAACGCGAAGAt < 1:205687/69‑1 (MQ=255) ccaGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGa > 1:2168210/1‑55 (MQ=255) ccaGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGa > 1:2775590/1‑55 (MQ=255) ccaGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGaa > 1:3257774/1‑64 (MQ=255) ccaGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGaa > 1:4008544/1‑64 (MQ=255) ccaGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGaa > 1:1309647/1‑64 (MQ=255) aGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCg < 1:655741/71‑1 (MQ=255) aGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCg > 1:2621311/1‑71 (MQ=255) aGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCg > 1:1328203/1‑71 (MQ=255) aGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGAt > 1:1391504/1‑65 (MQ=255) gCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGaaaa > 1:1133817/1‑51 (MQ=255) cGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:3294435/71‑1 (MQ=255) cGTTCGATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:1484582/71‑1 (MQ=255) cGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATg > 1:2667173/1‑71 (MQ=255) cGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATg > 1:599756/1‑71 (MQ=255) gATGTGCAGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGc < 1:3298638/71‑1 (MQ=255) cAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGaa > 1:1511165/1‑44 (MQ=255) cAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGaa > 1:2654798/1‑44 (MQ=255) cAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGaa > 1:3569091/1‑44 (MQ=255) cAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGaa > 1:345065/1‑44 (MQ=255) cAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGa < 1:2621182/71‑1 (MQ=255) cAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGa < 1:2328781/71‑1 (MQ=255) aGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGc < 1:737913/64‑1 (MQ=255) aGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg < 1:1592669/71‑1 (MQ=255) aGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg < 1:1490731/71‑1 (MQ=255) aGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg < 1:167653/71‑1 (MQ=255) aGATGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg < 1:1884146/71‑1 (MQ=255) aTGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg < 1:2866032/69‑1 (MQ=255) tGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTAt < 1:983760/71‑1 (MQ=255) tGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTAt < 1:1531039/71‑1 (MQ=255) tGCAAATCGTCCGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTAt > 1:2160065/1‑71 (MQ=255) gCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTa > 1:1284183/1‑69 (MQ=255) gCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTAt > 1:1771740/1‑70 (MQ=255) gCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTAt > 1:3238395/1‑70 (MQ=255) cAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATg < 1:2000508/70‑1 (MQ=255) aaTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCggg < 1:1314914/51‑1 (MQ=255) aaTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCggg < 1:1479349/51‑1 (MQ=255) aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATc > 1:1304548/1‑39 (MQ=255) aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCg > 1:1801663/1‑40 (MQ=255) aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCg > 1:3625590/1‑40 (MQ=255) aTCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCg > 1:42510/1‑40 (MQ=255) cGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGActtct > 1:2795324/1‑71 (MQ=255) cGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCg < 1:3539637/70‑1 (MQ=255) cGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCg < 1:1207525/70‑1 (MQ=255) cGCTCTATACAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCg < 1:3108005/70‑1 (MQ=255) ctctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAg < 1:3307874/55‑1 (MQ=255) ctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCGATTGAGTATGACTTCTTCGATCCt > 1:2074310/1‑71 (MQ=255) ctATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCt > 1:2345602/1‑71 (MQ=255) tGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGt < 1:1804538/38‑1 (MQ=255) | ATGAAATTTATCCGAACGGTATCTCCACCAGCCTGCCGTTCGATGTGCAGATGCAAATCGTCCGCTCTATGCAGGGGATGGAAAACGCGAAGATCGTGCGTCCGGGTTATGCCATTGAGTATGACTTCTTCGATCCT > W3110S.gb/3711963‑3712099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |