| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,714,423 | C→T | 14.3% | H61Y (CAC→TAC) | atpI → | ATP synthase, membrane‑bound accesory subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,714,423 | 0 | C | T | 14.3% | 71.7 / 4.6 | 28 | H61Y (CAC→TAC) | atpI | ATP synthase, membrane‑bound accesory subunit |
| Reads supporting (aligned to +/- strand): ref base C (15/9); new base T (2/2); total (17/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
TCTCTGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCA > W3110S.gb/3714355‑3714486 | tctcTGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc < 1:1713845/71‑1 (MQ=255)tctcTGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc < 1:1718635/71‑1 (MQ=255)tctcTGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCAc < 1:753125/71‑1 (MQ=255)tctcTGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCAc < 1:1508541/71‑1 (MQ=255) tctGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAcg > 1:2906382/1‑69 (MQ=255) tctGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCAc > 1:1788800/1‑69 (MQ=255) tGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAgg > 1:473462/1‑71 (MQ=255) aaTAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCa > 1:2144168/1‑66 (MQ=255) aTAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCa < 1:3445807/71‑1 (MQ=255) aTAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCa < 1:1884040/71‑1 (MQ=255) aGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATa > 1:1381978/1‑70 (MQ=255) ccTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCa > 1:1247957/1‑51 (MQ=255) ccTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCa > 1:2804118/1‑51 (MQ=255) ggCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAg > 1:1896265/1‑71 (MQ=255) aGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAg > 1:4003515/1‑49 (MQ=255) aGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAg > 1:2937070/1‑49 (MQ=255) gTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTc < 1:1221526/43‑1 (MQ=255) gTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTc < 1:1813336/43‑1 (MQ=255) tCTTTCTGCCTAACGTTTTCTTTATGATATTTGCCTGGCGTCAcc < 1:1704739/45‑1 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCAc > 1:2675015/1‑42 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGt > 1:2033863/1‑71 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGt > 1:277683/1‑71 (MQ=255) tAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGgcg < 1:71484/40‑1 (MQ=255) tAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGa < 1:2060453/53‑1 (MQ=255) aagTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACa > 1:2863323/3‑71 (MQ=255) aCGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGaaa > 1:2156774/1‑53 (MQ=255) aCGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCC‑‑GTGGCCTGGACAtt > 1:2475082/1‑71 (MQ=255) gTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACAtt < 1:2300216/71‑1 (MQ=255) tttGCCTGGCGTCACCAGGCGCATACACCAGCGAAAg > 1:2664927/1‑37 (MQ=255) cTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCa < 1:206546/71‑1 (MQ=255) | TCTCTGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCA > W3110S.gb/3714355‑3714486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |