| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,718,376 | G→A | 36.7% | intergenic (+9/‑42) | atpA → / → atpG | F1 sector of membrane‑bound ATP synthase, alpha subunit/F1 sector of membrane‑bound ATP synthase, gamma subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,718,376 | 0 | G | A | 36.7% | 44.2 / 49.1 | 49 | intergenic (+9/‑42) | atpA/atpG | F1 sector of membrane‑bound ATP synthase, alpha subunit/F1 sector of membrane‑bound ATP synthase, gamma subunit |
| Reads supporting (aligned to +/- strand): ref base G (18/13); new base A (11/7); total (29/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
CGAAATCGAAGGCAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCGGCGCAAAAGAGATACG > W3110S.gb/3718307‑3718440 | cgaAATCGAAGGCAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTggcgg < 1:989713/71‑1 (MQ=255) aaTCGAAGGCAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCtt < 1:1858702/71‑1 (MQ=255) aTCGAAGGCAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTg > 1:2262880/1‑71 (MQ=255) tCGAAGGCAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTggcg < 1:815270/65‑1 (MQ=255) tCGAAGGCAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTggcg < 1:1390424/65‑1 (MQ=255) tCGAAGGCAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTggcg < 1:2103058/65‑1 (MQ=255) gAAGGCAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCt < 1:3404438/71‑1 (MQ=255) gAAGGCAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCt < 1:1931814/71‑1 (MQ=255) gAAGGCAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGG‑GGCTTGCCtt < 1:2195539/71‑1 (MQ=255) aaGGCAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCAGCTTGCCtt < 1:3240335/71‑1 (MQ=255) cAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑ggg > 1:2510113/1‑71 (MQ=255) cAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑ggg > 1:2218203/1‑71 (MQ=255) aaGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGc < 1:2762175/71‑1 (MQ=255) aaGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGc < 1:963838/71‑1 (MQ=255) aaGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGc < 1:4045854/71‑1 (MQ=255) aaGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGc < 1:2547990/71‑1 (MQ=255) aGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTggcgg < 1:3102492/57‑1 (MQ=255) gCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCAGCTTGCCtt < 1:1410764/64‑1 (MQ=255) cTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGCAgg > 1:2024295/1‑71 (MQ=255) cTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGCAgg > 1:1522879/1‑71 (MQ=255) cTGAAAGGCATCCTCGATTCCTTAAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGCAgg > 1:2895182/1‑71 (MQ=255) gAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGCAGGcc > 1:3756259/1‑71 (MQ=255) gAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGCAGGcc > 1:2801108/1‑71 (MQ=255) aaGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGCAGGCCGc < 1:2858675/71‑1 (MQ=255) aGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTggcggc > 1:1113772/1‑51 (MQ=255) tacTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCAGCTTGCCTTa < 1:3567790/52‑1 (MQ=255) tCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCtt < 1:1041800/48‑1 (MQ=255) tCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCtt < 1:3952540/48‑1 (MQ=255) tCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGCAGGCCGCAAGGc > 1:2287391/1‑70 (MQ=255) tCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCAGCTTGCCTTa < 1:1873289/54‑1 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTggcgg > 1:1077711/1‑43 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTggcgg > 1:3730387/1‑43 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTggcagc > 1:1191007/1‑44 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTggcagc > 1:3914425/1‑44 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGcc > 1:306870/1‑49 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGcc > 1:3273883/1‑49 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGcc > 1:1311090/1‑49 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTAGGGGCAGGCCGCAAGGCAt > 1:148422/1‑71 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGCAGGCCGCaa > 1:1981856/1‑65 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGCAGGCCGCAAGGCAtt > 1:2346259/1‑71 (MQ=255) cTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGCAGGCCGCAAGGCAtt > 1:2125950/1‑71 (MQ=255) tCCTTCTAAGCAACCCAATCCTGGTAACGTCTGGCAGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTgag > 1:527593/1‑68 (MQ=255) tCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCAGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTgag > 1:1035531/1‑68 (MQ=255) tCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCAGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTgag > 1:873099/1‑68 (MQ=255) tCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCAGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTgag > 1:372424/1‑68 (MQ=255) cAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCt > 1:765221/1‑71 (MQ=255) aaaGCAACCCAATCCTGGTAACGTCTGGCGGCTGGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCt < 1:527701/70‑1 (MQ=255) aGCAACCCAATCCTGGTAACGTCTGGCAGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCTCAt < 1:790595/71‑1 (MQ=255) aGCAACCCAATCCTGGTAACGTCTGCCAGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCTCAt < 1:1928609/71‑1 (MQ=255) aaCCCAATCCTGGTAACGTCTGGCAGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGc > 1:2218126/1‑71 (MQ=255) aaCCCAATCCTGGTAACGTCTGGCAGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGc > 1:1899185/1‑71 (MQ=255) aaCCCAATCCTGGTAACGTCTGGCAGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGc > 1:304391/1‑71 (MQ=255) cAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCGgc > 1:2908647/1‑71 (MQ=255) cAATCCTGGTAACGTCTGGCAGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCgg > 1:505170/1‑70 (MQ=255) cAATCCTGGTAACGTCTGGCAGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCgg > 1:2975387/1‑70 (MQ=255) cAATCCTGGTAACGTCTGGCAGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCGgc > 1:3869013/1‑71 (MQ=255) cTGGTAACGTCTGGCGGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCGGCGCaa < 1:2011770/70‑1 (MQ=255) cTGGTAACGTCTGGCAGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCGGCGCaa < 1:2007549/70‑1 (MQ=255) tGGCAGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCGGCGCAAAAGAGATACg > 1:808442/1‑69 (MQ=255) | CGAAATCGAAGGCAAGCTGAAAGGCATCCTCGATTCCTTCAAAGCAACCCAATCCTGGTAACGTCTGGCGGCTTGCCTTA‑GGGCAGGCCGCAAGGCATTGAGGAGAAGCTCATGGCCGGCGCAAAAGAGATACG > W3110S.gb/3718307‑3718440 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |