| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,730,340 | C→T | 7.9% | T75T (ACC→ACT) | bglG → | transcriptional antiterminator of the bgl operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,730,340 | 0 | C | T | 7.9% | 275.0 / 10.3 | 89 | T75T (ACC→ACT) | bglG | transcriptional antiterminator of the bgl operon |
| Reads supporting (aligned to +/- strand): ref base C (29/53); new base T (4/3); total (33/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
GTCATGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTTATATCTCGCTAA > W3110S.gb/3730276‑3730410 | gTCATGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGAt > 1:2821896/1‑71 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg < 1:3629041/70‑1 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg < 1:3183446/70‑1 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg < 1:3066987/70‑1 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg > 1:2861177/1‑70 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg > 1:1951302/1‑70 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg < 1:1328333/70‑1 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg > 1:2161289/1‑70 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg > 1:2553602/1‑70 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg < 1:3847857/70‑1 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg < 1:2258344/70‑1 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg < 1:533951/70‑1 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg > 1:886890/1‑70 (MQ=255) aTGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCg < 1:2242801/70‑1 (MQ=255) tgaactgaacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACTTGTGATCg < 1:1088135/69‑1 (MQ=255) aactgaacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGtatt < 1:3190745/71‑1 (MQ=255) aactgaacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGtatt < 1:2231491/71‑1 (MQ=255) actgaacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGtatt < 1:3313994/70‑1 (MQ=255) ctgaacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGtatt < 1:2572826/69‑1 (MQ=255) tgaacGGGCGATTAAGCGCACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACTTGTGATCg < 1:3869906/64‑1 (MQ=255) tgaacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACTTGTGATCg < 1:969956/64‑1 (MQ=255) gaacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGtattat > 1:1425562/1‑69 (MQ=255) gaacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGtattat > 1:2693296/1‑69 (MQ=255) gaacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTAtct > 1:1496563/1‑71 (MQ=255) aacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACTTGTGATCGta > 1:3045493/1‑64 (MQ=255) aacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACTTGTGATCGta > 1:3191334/1‑64 (MQ=255) aacGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACTTGTGATCGta > 1:4073039/1‑64 (MQ=255) acGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGAt > 1:1001725/1‑59 (MQ=255) acGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGAt > 1:3597504/1‑59 (MQ=255) cGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCtt < 1:3621662/71‑1 (MQ=255) cGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCtt < 1:2868769/71‑1 (MQ=255) cGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCtt < 1:3541183/71‑1 (MQ=255) gATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAgcg < 1:3822147/71‑1 (MQ=255) gATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAgcg < 1:2540526/71‑1 (MQ=255) gATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAgcg < 1:2545385/71‑1 (MQ=255) aTTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGt < 1:2445616/55‑1 (MQ=255) aTTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTAtct < 1:1316254/62‑1 (MQ=255) ttAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAgcg > 1:2441244/1‑69 (MQ=255) tAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGta > 1:476749/1‑54 (MQ=255) tAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAg > 1:1738522/1‑71 (MQ=255) aaGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAg > 1:922392/1‑70 (MQ=255) aGCGAACTCTTAAGTCATATTGCTCTTGAGGTGATGGCAACCTGTGTTCTTATTATCTCTTTAGCGCAgg < 1:3661659/70‑1 (MQ=255) aGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAgg < 1:1333932/70‑1 (MQ=255) gAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAgc < 1:3921845/70‑1 (MQ=255) aCTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACTTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCt > 1:3873345/1‑71 (MQ=255) aCTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCt < 1:2735564/71‑1 (MQ=255) aCTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCt < 1:612452/71‑1 (MQ=255) ctctTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCtt < 1:2746701/71‑1 (MQ=255) aaGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATctct > 1:4052353/1‑49 (MQ=255) aaGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGa > 1:2227999/1‑70 (MQ=255) aGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGaaa < 1:1002336/71‑1 (MQ=255) aGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGaaa < 1:97479/71‑1 (MQ=255) aGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGaaa < 1:984488/71‑1 (MQ=255) gTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCtt < 1:1102783/48‑1 (MQ=255) gTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCtt < 1:1233742/48‑1 (MQ=255) gTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCtt < 1:1056535/48‑1 (MQ=255) tCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGtatt < 1:2994558/40‑1 (MQ=255) tCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAAt < 1:3286335/71‑1 (MQ=255) atatTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAAtt < 1:2205193/70‑1 (MQ=255) atatTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTa < 1:516506/71‑1 (MQ=255) atatTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTa < 1:1682994/71‑1 (MQ=255) atatTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTa > 1:442109/1‑71 (MQ=255) tatTCCTCTTGAGGTGATGGCAACCTGTGATCGtat < 1:3490807/36‑1 (MQ=255) tatTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCtt < 1:1087112/44‑1 (MQ=255) tatTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAgcgc < 1:3419192/50‑1 (MQ=255) atTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGaaaa > 1:2507684/1‑66 (MQ=255) ttCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCATGAGCGCTTGGGAAAATTACAg < 1:410794/71‑1 (MQ=255) ttCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAg > 1:821555/1‑71 (MQ=255) ttCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAg < 1:2092630/71‑1 (MQ=255) ttCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAg < 1:1237651/71‑1 (MQ=255) ttCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAg < 1:485568/71‑1 (MQ=255) ttCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAg < 1:181378/71‑1 (MQ=255) ccTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGa > 1:3941283/1‑71 (MQ=255) ctctTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGAc < 1:2316005/71‑1 (MQ=255) ctctTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGAc < 1:787374/71‑1 (MQ=255) aGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTAtt < 1:995193/71‑1 (MQ=255) aGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTAtc < 1:1000048/71‑2 (MQ=255) gTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTta < 1:3567310/71‑1 (MQ=255) tGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTtat < 1:3856561/71‑1 (MQ=255) tGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTtat < 1:1979156/71‑1 (MQ=255) aTGGCAACCTGTTATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTtat > 1:1596019/1‑69 (MQ=255) aTGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTa > 1:1004084/1‑64 (MQ=255) aTGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTa > 1:1457044/1‑64 (MQ=255) aTGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTa > 1:150741/1‑64 (MQ=255) aTGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTtata > 1:629449/1‑70 (MQ=255) ggCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAg > 1:3798786/1‑55 (MQ=255) ggCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAg > 1:2377855/1‑55 (MQ=255) gCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTTATAtctc > 1:3138382/1‑71 (MQ=255) gCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTTATAtctc < 1:3076350/71‑1 (MQ=255) ccTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTTCAGGACAGTATTTATATCTCGCt > 1:3368264/1‑70 (MQ=255) ccTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTtat > 1:543852/1‑62 (MQ=255) ccTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTta < 1:810922/61‑1 (MQ=255) ccTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTta < 1:393111/61‑1 (MQ=255) ccTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTTATATCTCGCt > 1:717356/1‑70 (MQ=255) cTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTTATATCTCGCTaa > 1:3840567/1‑71 (MQ=255) | GTCATGAACTGAACGGGCGATTAAGCGAACTCTTAAGTCATATTCCTCTTGAGGTGATGGCAACCTGTGATCGTATTATCTCTTTAGCGCAGGAGCGCTTGGGAAAATTACAGGACAGTATTTATATCTCGCTAA > W3110S.gb/3730276‑3730410 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |