| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,748,215 | G→A | 31.7% | S256S (AGC→AGT) | tnaB ← | tryptophan transporter of low affinity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,748,215 | 0 | G | A | 31.7% | 52.8 / 33.1 | 41 | S256S (AGC→AGT) | tnaB | tryptophan transporter of low affinity |
| Reads supporting (aligned to +/- strand): ref base G (10/18); new base A (8/5); total (18/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGTTTGGTGCCGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATAAATCACCAGCGCC > W3110S.gb/3748146‑3748275 | cTGTTTGGTGCCGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGc > 1:2352442/1‑71 (MQ=255) tttGGTGCCGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCtt < 1:101062/70‑1 (MQ=255) tttGGTGCCGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAACt < 1:25252/69‑1 (MQ=255) tGGTGCCGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAACTTtct > 1:3105960/1‑71 (MQ=255) tGGTGCCGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAACTTtct > 1:3656872/1‑71 (MQ=255) ccGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCgg < 1:3697316/71‑1 (MQ=255) ccGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAACTTTCTCGCgg < 1:1018569/71‑1 (MQ=255) ccGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAACTTTCTCGCgg < 1:1193202/71‑1 (MQ=255) gAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGaa < 1:953192/71‑1 (MQ=255) aGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGaa < 1:3846054/70‑1 (MQ=255) ggAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAata < 1:3069670/71‑1 (MQ=255) gAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGc < 1:33845/56‑1 (MQ=255) gAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAata > 1:2934759/1‑70 (MQ=255) aaCGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAACTTTCTCGCGGAATAtt < 1:3588269/71‑1 (MQ=255) aTTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAATATTccc > 1:2556103/1‑70 (MQ=255) aTTTCACCAGCGAATCAACGTTGCCGCCT‑‑GGAGATAATCGCCTTAAAGCtt < 1:91114/51‑1 (MQ=37) tttCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGaa < 1:2709265/62‑1 (MQ=255) cAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGc < 1:249777/71‑1 (MQ=255) aGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGaa < 1:966057/55‑1 (MQ=255) aGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGc > 1:3962973/1‑70 (MQ=255) cGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAACTTTCTCGCGGAata > 1:1228353/1‑55 (MQ=255) cGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAACTTTCTCGCGGAata > 1:628938/1‑55 (MQ=255) cGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAACTTTCTCGCGGAata > 1:2374875/1‑55 (MQ=255) gAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCaa > 1:1902328/1‑69 (MQ=255) gAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCaa > 1:1359720/1‑69 (MQ=255) tCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGaa < 1:3302424/49‑1 (MQ=255) tCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAACTTTCTCGCGTAATATTCCCCATGGTGCAATagag > 1:2872248/1‑71 (MQ=255) cGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCag < 1:2161752/71‑1 (MQ=255) cGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCag < 1:1715273/71‑1 (MQ=255) gagaTAATCGCCTTAAAACTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATAAATCAc < 1:1869114/71‑1 (MQ=255) agaTAATCGCCTTAATGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATAAATCAc < 1:1823345/70‑1 (MQ=255) aTAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAAt > 1:307251/1‑46 (MQ=255) aTAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCagaaga < 1:3041461/58‑1 (MQ=255) aaTCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAgaga > 1:479435/1‑58 (MQ=255) aTCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATa > 1:1181762/1‑59 (MQ=255) tggCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATAAATCACCAGCGcc < 1:3323177/69‑1 (MQ=255) gCCTTAAAACTTTCTCGCGGAATATTCCCCATGGTGCAATa > 1:642452/1‑41 (MQ=255) gCCTTAAAACTTTCTCGCGGAATATTCCCCATGGTGCAATa > 1:2550455/1‑41 (MQ=255) ccTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCa < 1:1301168/37‑1 (MQ=255) ccTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCa < 1:2614374/37‑1 (MQ=255) aaaaCTTTCTCGCGGAATATTCCCCATGGTGCAAt > 1:3237445/1‑35 (MQ=255) aaaaCTTTCTCGCGGAATATTCCCCATGGTGCAAt > 1:1935817/1‑35 (MQ=255) aaGATTTCTCGCGGAATATTCCCCATGGTGCAATa > 1:2252837/1‑35 (MQ=255) | CTGTTTGGTGCCGAGGAACGATTTCACCAGCGAATCAACGTTGCCGCCTGAGGAGATAATCGCCTTAAAGCTTTCTCGCGGAATATTCCCCATGGTGCAATAGAGCCAGAAGAGATAAATCACCAGCGCC > W3110S.gb/3748146‑3748275 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |