| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 301,283 | A→C | 21.6% | V172G (GTG→GGG) | yagT ← | predicted xanthine dehydrogenase, 2Fe‑2S subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 301,283 | 0 | A | C | 21.6% | 47.9 / 20.4 | 37 | V172G (GTG→GGG) | yagT | predicted xanthine dehydrogenase, 2Fe‑2S subunit |
| Reads supporting (aligned to +/- strand): ref base A (15/14); new base C (2/6); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGC > W3110S.gb/301217‑301353 | gttTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCa > 1:627647/1‑67 (MQ=255) tCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCCCCGCTAc > 1:433172/1‑71 (MQ=255) tCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTAc > 1:1253516/1‑71 (MQ=255) tCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTAc > 1:2432338/1‑71 (MQ=255) gAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACt < 1:2250849/68‑1 (MQ=255) aGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACtgatg < 1:178638/71‑1 (MQ=255) aaCCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCCCCGCTACTGATGAGCaaa < 1:855713/71‑1 (MQ=255) aaCCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCCCCGCTACTGATGAGCaa < 1:2851065/70‑1 (MQ=255) aCCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCaaa > 1:3359676/1‑70 (MQ=255) cAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAAttt < 1:964934/71‑1 (MQ=255) aCCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGa < 1:2961091/71‑1 (MQ=255) tgacTGGGAATGCCGTCCTGAATCTCTTTTAGCCCCGCTACTGATGAGCaaa < 1:885635/52‑1 (MQ=255) cTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCa < 1:1926755/65‑1 (MQ=255) gggAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGt > 1:3789536/1‑65 (MQ=255) ccGTCCTGAATCTCTTTTAGCACAGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTgg > 1:1120666/1‑69 (MQ=255) tCCTGAATCTCTTTTAGCACCGCTACTGATGAGCaaa < 1:759111/37‑1 (MQ=255) cTGAATCTCTTTTAGCACCGCTACTGATGAGCAAAtt > 1:3901721/1‑37 (MQ=255) cTGAATCTCTTTTAGCACCGCTACTGATGAGCAAAtt > 1:3171983/1‑37 (MQ=255) cTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcat < 1:3890173/71‑1 (MQ=255) cTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcat > 1:1200576/1‑71 (MQ=255) cTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcat > 1:3038542/1‑71 (MQ=255) gAATCTCTTTTAGCACCGCTACTGATGAGCAATTTTGCCCGGAGGTGc < 1:2709208/48‑1 (MQ=255) gAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGc < 1:1588359/48‑1 (MQ=255) gAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcatca > 1:2866443/1‑71 (MQ=255) gAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcatca > 1:3181042/1‑71 (MQ=255) aaTCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGc < 1:182808/47‑1 (MQ=255) tctcTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCAc < 1:3699645/56‑1 (MQ=255) tctTTTAGCCCCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCtt > 1:228946/1‑71 (MQ=255) ttttAGCCCCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCtt < 1:1867395/69‑1 (MQ=255) ttAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcatca > 1:2925428/1‑62 (MQ=255) ttAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcatca > 1:2538124/1‑62 (MQ=255) ttAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGAt > 1:600943/1‑70 (MQ=255) ttAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGAt > 1:1928581/1‑70 (MQ=255) tAGCCCCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATa < 1:3586468/70‑1 (MQ=255) tAGCCCCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATa < 1:4069889/70‑1 (MQ=255) aGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATaa < 1:3375163/70‑1 (MQ=255) aGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATaa < 1:3528820/70‑1 (MQ=255) gCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATaa < 1:3926742/69‑1 (MQ=255) aCCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGgc < 1:3651044/71‑1 (MQ=255) | GTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGC > W3110S.gb/301217‑301353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |