Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,824,362 A→T 100% pseudogene (495/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,3620AT100.0% 185.4 / NA 63pseudogene (495/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (31/32);  total (31/32)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCTGGTGGAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGA  >  W3110S.gb/3824295‑3824424
                                                                   |                                                              
aGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTAtt                                                             >  1:3641157/1‑71 (MQ=255)
aGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTAtt                                                             >  1:2333468/1‑71 (MQ=255)
aGCTGGTGGAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTAt                                                              <  1:3849773/70‑1 (MQ=255)
      tggAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAAc                                                       <  1:3459786/71‑1 (MQ=255)
               gCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGc                                               >  1:3943012/1‑70 (MQ=255)
                     tGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGc                                        >  1:610660/1‑71 (MQ=255)
                       aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCg                                       <  1:3496848/70‑1 (MQ=255)
                       aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCg                                       <  1:350950/70‑1 (MQ=255)
                          aCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCg                                       <  1:3361217/67‑1 (MQ=255)
                            cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                 <  1:1021298/71‑1 (MQ=255)
                            cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                 <  1:2033232/71‑1 (MQ=255)
                            cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGGCGGTATTGTAACCGGTGAAGCAATTTGCGATCTgg                                 <  1:3730322/71‑1 (MQ=255)
                            cAACCCGATGAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                 <  1:1766045/70‑1 (MQ=255)
                              acccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                               <  1:606789/69‑1 (MQ=255)
                               cccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCg                                       <  1:3612276/61‑1 (MQ=255)
                                ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa                                      >  1:211847/1‑62 (MQ=255)
                                ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGAt                                     >  1:2002473/1‑63 (MQ=255)
                                ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGAt                                     >  1:1648232/1‑63 (MQ=255)
                                ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGAt                                     >  1:2570181/1‑63 (MQ=255)
                                    tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATtgta                                                          <  1:1766153/38‑2 (MQ=38)
                                    tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATtgta                                                          <  1:1326989/38‑2 (MQ=38)
                                    tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATtgta                                                          <  1:3038544/38‑2 (MQ=38)
                                    tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATtgta                                                          <  1:2985767/38‑2 (MQ=38)
                                    tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATa                             <  1:2110420/67‑1 (MQ=255)
                                    tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATa                             <  1:4010269/67‑1 (MQ=255)
                                    tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGCAATa                             <  1:1312683/67‑1 (MQ=255)
                                     gAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                         >  1:3640234/1‑70 (MQ=255)
                                       aaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                      <  1:695342/71‑1 (MQ=255)
                                         gggATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                               >  1:2627856/1‑60 (MQ=255)
                                         gggATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGa                    >  1:3939322/1‑71 (MQ=255)
                                            aTGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                 <  1:2792880/55‑1 (MQ=255)
                                             tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                              <  1:3073153/41‑2 (MQ=37)
                                             tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                              <  1:2779632/41‑2 (MQ=37)
                                             tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                              <  1:3927718/41‑2 (MQ=37)
                                             tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                              <  1:2212050/41‑2 (MQ=37)
                                             tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                              <  1:3548620/41‑2 (MQ=37)
                                             tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                              <  1:93076/41‑2 (MQ=37)
                                             tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                              <  1:1062499/41‑2 (MQ=37)
                                               gTAGCCTCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                      <  1:550/63‑1 (MQ=255)
                                               gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCg                                       <  1:2418701/46‑1 (MQ=25)
                                               gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                      >  1:4070183/1‑63 (MQ=255)
                                               gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                      >  1:681364/1‑63 (MQ=255)
                                               gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                      >  1:3337982/1‑63 (MQ=255)
                                               gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                      >  1:3494366/1‑63 (MQ=255)
                                               gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc              >  1:2409979/1‑71 (MQ=255)
                                                tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa                                      >  1:841309/1‑46 (MQ=25)
                                                tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa                                      >  1:1793118/1‑46 (MQ=25)
                                                tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa                                      >  1:1723619/1‑46 (MQ=25)
                                                tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGcc             >  1:3556558/1‑71 (MQ=255)
                                                tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGcc             >  1:3234946/1‑71 (MQ=255)
                                                  gCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg            <  1:363868/70‑1 (MQ=255)
                                                   ccGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa          <  1:3884274/71‑1 (MQ=255)
                                                   ccGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa          <  1:3462913/71‑1 (MQ=255)
                                                    cGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg            >  1:975391/1‑68 (MQ=255)
                                                          ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGAcc    >  1:3221884/1‑70 (MQ=255)
                                                           tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGa                                >  1:3889590/1‑41 (MQ=19)
                                                           tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa  >  1:2740603/1‑71 (MQ=255)
                                                           tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa  >  1:107740/1‑71 (MQ=255)
                                                           tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa  >  1:806512/1‑71 (MQ=255)
                                                           tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa  >  1:1253759/1‑71 (MQ=255)
                                                           tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa  >  1:2713519/1‑71 (MQ=255)
                                                           tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa  >  1:1003676/1‑71 (MQ=255)
                                                           tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa  >  1:3724334/1‑71 (MQ=255)
                                                                   |                                                              
AGCTGGTGGAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGA  >  W3110S.gb/3824295‑3824424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: