Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,824,368 G→A 100% pseudogene (501/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,3680GA100.0% 188.2 / NA 64pseudogene (501/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/33);  total (31/33)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGGAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACG  >  W3110S.gb/3824301‑3824432
                                                                   |                                                                
tggAAAACGGCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAAc                                                               <  1:3459786/71‑1 (MQ=255)
         gCAAGCTGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGc                                                       >  1:3943012/1‑70 (MQ=255)
               tGAAAACCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGc                                                >  1:610660/1‑71 (MQ=255)
                 aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCg                                               <  1:350950/70‑1 (MQ=255)
                 aaaaCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCg                                               <  1:3496848/70‑1 (MQ=255)
                    aCCAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCg                                               <  1:3361217/67‑1 (MQ=255)
                      cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                         <  1:1021298/71‑1 (MQ=255)
                      cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                         <  1:2033232/71‑1 (MQ=255)
                      cAACCCGATGAAAGGGATGGTAGCCGCAGTTTCTGGCGGTATTGTAACCGGTGAAGCAATTTGCGATCTgg                                         <  1:3730322/71‑1 (MQ=255)
                      cAACCCGATGAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                         <  1:1766045/70‑1 (MQ=255)
                        acccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                       <  1:606789/69‑1 (MQ=255)
                         cccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCg                                               <  1:3612276/61‑1 (MQ=255)
                          ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa                                              >  1:211847/1‑62 (MQ=255)
                          ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGAt                                             >  1:1648232/1‑63 (MQ=255)
                          ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGAt                                             >  1:2002473/1‑63 (MQ=255)
                          ccGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGAt                                             >  1:2570181/1‑63 (MQ=255)
                              tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATa                                     <  1:2110420/67‑1 (MQ=255)
                              tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATa                                     <  1:4010269/67‑1 (MQ=255)
                              tGAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGCAATa                                     <  1:1312683/67‑1 (MQ=255)
                               gAAAGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                 >  1:3640234/1‑70 (MQ=255)
                                 aaGGGATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                              <  1:695342/71‑1 (MQ=255)
                                   gggATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGaa                                       >  1:2627856/1‑60 (MQ=255)
                                   gggATGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGa                            >  1:3939322/1‑71 (MQ=255)
                                      aTGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTgg                                         <  1:2792880/55‑1 (MQ=255)
                                       tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                      <  1:2779632/41‑2 (MQ=37)
                                       tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                      <  1:3073153/41‑2 (MQ=37)
                                       tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                      <  1:3927718/41‑2 (MQ=37)
                                       tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                      <  1:2212050/41‑2 (MQ=37)
                                       tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                      <  1:3548620/41‑2 (MQ=37)
                                       tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                      <  1:93076/41‑2 (MQ=37)
                                       tGGTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGca                                                      <  1:1062499/41‑2 (MQ=37)
                                         gTAGCCTCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                              <  1:550/63‑1 (MQ=255)
                                         gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCg                                               <  1:2418701/46‑1 (MQ=25)
                                         gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                              >  1:681364/1‑63 (MQ=255)
                                         gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                              >  1:3337982/1‑63 (MQ=255)
                                         gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                              >  1:4070183/1‑63 (MQ=255)
                                         gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGaa                              >  1:3494366/1‑63 (MQ=255)
                                         gTAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGc                      >  1:2409979/1‑71 (MQ=255)
                                          tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa                                              >  1:841309/1‑46 (MQ=25)
                                          tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa                                              >  1:1793118/1‑46 (MQ=25)
                                          tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGa                                              >  1:1723619/1‑46 (MQ=25)
                                          tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGcc                     >  1:3556558/1‑71 (MQ=255)
                                          tAGCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGcc                     >  1:3234946/1‑71 (MQ=255)
                                            gCCGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg                    <  1:363868/70‑1 (MQ=255)
                                             ccGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                  <  1:3462913/71‑1 (MQ=255)
                                             ccGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                  <  1:3884274/71‑1 (MQ=255)
                                              cGCAGTTTCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCg                    >  1:975391/1‑68 (MQ=255)
                                                    ttCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGAcc            >  1:3221884/1‑70 (MQ=255)
                                                     tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGa                                        >  1:3889590/1‑41 (MQ=19)
                                                     tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa          >  1:1003676/1‑71 (MQ=255)
                                                     tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa          >  1:107740/1‑71 (MQ=255)
                                                     tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa          >  1:1253759/1‑71 (MQ=255)
                                                     tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa          >  1:806512/1‑71 (MQ=255)
                                                     tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa          >  1:2713519/1‑71 (MQ=255)
                                                     tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa          >  1:2740603/1‑71 (MQ=255)
                                                     tCTGTCGGTATTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGa          >  1:3724334/1‑71 (MQ=255)
                                                           ggtaTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGaa    >  1:1427256/4‑71 (MQ=255)
                                                             taTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                  <  1:625363/54‑1 (MQ=255)
                                                             taTTGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCa                  <  1:3338627/54‑1 (MQ=255)
                                                               ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                 <  1:2484554/38‑1 (MQ=21)
                                                               ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                 <  1:2361327/38‑1 (MQ=21)
                                                               ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                 <  1:2071848/38‑1 (MQ=21)
                                                               ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGtt                                 <  1:1858261/38‑1 (MQ=21)
                                                               ttGTAAACGGTGAAGCAATTTGCGATCTGGAATACGTTGAAGACTCAGCCGCAGAGACCGACATGAACg  >  1:1263306/1‑69 (MQ=255)
                                                                tgtaAACGGTGAAGCAATTTGCGATCTGGAATTCGtt                                 <  1:3684138/37‑1 (MQ=12)
                                                                tgtaAACGGTCAAGCAATTTGCGATCTGGAATACGtt                                 <  1:602387/37‑1 (MQ=12)
                                                                   |                                                                
TGGAAAACGGCAAGCTGAAAACCAATCCGATGAAAGGGATGGTAGCCGCAGTTTCTGTCGGAATTGTGAACGGCGAAGCGGTTTGCGATCTGGAATACGTTGAAGACTCTGCCGCAGAGACCGACATGAACG  >  W3110S.gb/3824301‑3824432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: