| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 306,593 | T→C | 44.7% | N655S (AAC→AGC) | yagX ← | predicted aromatic compound dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 306,593 | 0 | T | C | 44.7% | 6.6 / 49.2 | 38 | N655S (AAC→AGC) | yagX | predicted aromatic compound dioxygenase |
| Reads supporting (aligned to +/- strand): ref base T (13/8); new base C (4/13); total (17/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.47e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCGTCGTCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATT > W3110S.gb/306523‑306663 | aCCGTCGTCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGt > 1:1508628/1‑71 (MQ=255) gtcCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGCTTTTAc < 1:2846106/71‑1 (MQ=255) gtcCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGCTTTTAc < 1:2978666/71‑1 (MQ=255) gtcCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGCTTTTAc < 1:1316627/71‑1 (MQ=255) gtcCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGCTTTTAc < 1:3661824/71‑1 (MQ=255) ccAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTa > 1:3566625/1‑65 (MQ=255) gtgtTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGCTTTTACCCTGCCAGCCGAc < 1:4069697/71‑1 (MQ=255) gtTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGCTTTTACCCTGCCAGCCGACGc > 1:1892798/1‑71 (MQ=255) ttGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtttt < 1:1036363/52‑1 (MQ=255) ttGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtt < 1:3836604/50‑1 (MQ=255) gAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGc > 1:2426114/1‑71 (MQ=255) gAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGc > 1:3973315/1‑71 (MQ=255) gAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGc > 1:104612/1‑71 (MQ=255) aaTATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGcc < 1:1436318/71‑1 (MQ=255) tatCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtttt < 1:2479302/47‑1 (MQ=255) ccAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGt > 1:687961/1‑71 (MQ=255) gCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGCCa < 1:3234384/60‑1 (MQ=255) gCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGCCa < 1:2939235/60‑1 (MQ=255) cGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtttt > 1:1822193/1‑36 (MQ=255) cGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtttt > 1:544627/1‑36 (MQ=255) cGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtttt > 1:2079081/1‑36 (MQ=255) cGTTGCCATCAGTCCGCCCGCTGGCAGCAATGtttt > 1:240409/1‑36 (MQ=255) ttGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGtt > 1:3689678/1‑71 (MQ=255) gCCATCAGTCCGCCCGCTGGCAGCAATGTTCTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTgg > 1:2657923/1‑71 (MQ=255) ccATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTgg < 1:3874174/70‑1 (MQ=255) ccATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGt < 1:2529328/71‑1 (MQ=255) ccATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGt < 1:1168841/71‑1 (MQ=255) ccATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGt < 1:3365970/71‑1 (MQ=255) ccATCAGTCCGCCCGCTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTgg < 1:935587/70‑1 (MQ=255) ccATCAGTCCGCCCGCTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTgg < 1:912041/70‑1 (MQ=255) ccATCAGTCCGCCCGCTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTgg < 1:481100/70‑1 (MQ=255) tCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTAtt > 1:2077784/1‑71 (MQ=255) cAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTAtt > 1:3142095/1‑70 (MQ=255) aGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGa > 1:1977493/1‑71 (MQ=255) aGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGa > 1:1460433/1‑71 (MQ=255) aGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGa > 1:1484242/1‑71 (MQ=255) aGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGa > 1:1575462/1‑71 (MQ=255) aGTCCGCCCGCTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGa < 1:26479/71‑1 (MQ=255) cgcccgcTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACg > 1:1835795/1‑69 (MQ=255) cgcccgcTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACg > 1:3621446/1‑69 (MQ=255) cTGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTc < 1:3684632/71‑1 (MQ=255) tGGCAGCAATGCTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACg < 1:1475376/62‑1 (MQ=255) gcagcaATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTccgc > 1:3337509/1‑71 (MQ=255) gcaATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGa < 1:3606134/55‑1 (MQ=255) aaTGTTTTTACCCTGCCAGCCGACGCTGACATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCgcgc < 1:2301690/71‑1 (MQ=255) tGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTc < 1:201068/38‑1 (MQ=255) tttttACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTAtt < 1:3032673/47‑1 (MQ=255) tttttACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTAtt > 1:401811/1‑71 (MQ=255) tttttACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTAtt > 1:1147200/1‑71 (MQ=255) | ACCGTCGTCCTCCAGCCCGGTGTTGAATATCACCCCAGCGTTGCCATCAGTCCGCCCGCTGGCAGCAATGTTTTTACCCTGCCAGCCGACGCTGCCATTGGCGGTCAAGTTGGTATTGACGTAGCCGTCCGCCGCGCTATT > W3110S.gb/306523‑306663 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |