Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,824,524 T→G 100% pseudogene (657/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,5240TG100.0% 135.6 / NA 41pseudogene (657/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/14);  total (27/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGAGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGATTTTT  >  W3110S.gb/3824459‑3824588
                                                                 |                                                                 
atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc                                                              >  1:1093123/1‑71 (MQ=255)
atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc                                                              >  1:1633637/1‑71 (MQ=255)
atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc                                                              >  1:1586438/1‑71 (MQ=255)
   gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc                                                            >  1:473461/1‑70 (MQ=255)
         cAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAg                                                        >  1:461268/1‑67 (MQ=255)
          aGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAggggga                                                   <  1:723407/71‑3 (MQ=255)
          aGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAggggga                                                   <  1:2357366/71‑3 (MQ=255)
                  gCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcgaatc                                            <  1:821239/71‑1 (MQ=255)
                  gCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcgaatc                                            <  1:1714270/71‑1 (MQ=255)
                     gAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAggggg                                                    >  1:1675742/1‑58 (MQ=255)
                     gAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAggggg                                                    >  1:3509509/1‑58 (MQ=255)
                     gAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAggggg                                                    >  1:2550553/1‑58 (MQ=255)
                     gAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAggggg                                                    >  1:2595932/1‑58 (MQ=255)
                        ggCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCg                                                           <  1:3919844/50‑1 (MQ=255)
                        ggCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCg                                                           <  1:3809537/50‑1 (MQ=255)
                        ggCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCg                                                           <  1:1996183/50‑1 (MQ=255)
                        ggCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTg                                       >  1:3641532/1‑70 (MQ=255)
                        ggCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTg                                       >  1:667357/1‑70 (MQ=255)
                        ggCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTg                                       >  1:1984047/1‑70 (MQ=255)
                        ggCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGt                                      <  1:1503848/71‑1 (MQ=255)
                        ggCGAGCCGTTCACCCATGAAGAGATACTCACCTTGTTGGCGCTGGACCGAgcg                                                      >  1:3489216/1‑52 (MQ=255)
                              ccGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGAc                                >  1:2986264/1‑71 (MQ=255)
                              ccGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGAc                                >  1:755158/1‑71 (MQ=255)
                               cGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACg                               <  1:3033738/71‑1 (MQ=255)
                                   cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAg                            >  1:727954/1‑70 (MQ=255)
                                        aTGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAggcg                      >  1:2727104/1‑71 (MQ=255)
                                         tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCAt                                         <  1:1281463/51‑1 (MQ=255)
                                         tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAg                            <  1:2281106/64‑1 (MQ=255)
                                         tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAggcg                      >  1:3707253/1‑70 (MQ=255)
                                         tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCAGTGTAGc                                   <  1:523119/57‑1 (MQ=255)
                                          gAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAggcggc                    >  1:1166520/1‑71 (MQ=255)
                                           aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGgcggcg                   >  1:3269975/1‑71 (MQ=255)
                                           aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGgcggcg                   >  1:1172906/1‑71 (MQ=255)
                                             gagCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTg                                       >  1:4076294/1‑49 (MQ=255)
                                             gagCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTg                                       >  1:784009/1‑49 (MQ=255)
                                                  aCTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGa                                 >  1:958847/1‑50 (MQ=255)
                                                      accTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGc                                   >  1:819289/3‑44 (MQ=37)
                                                      accTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACt         >  1:617599/3‑70 (MQ=255)
                                                      accTTGCTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACt         >  1:1618231/3‑70 (MQ=255)
                                                         ttgttgGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCa                             <  1:1941479/47‑1 (MQ=255)
                                                            ttgGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGAttttt  <  1:549979/71‑1 (MQ=255)
                                                                 |                                                                 
ATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGAGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCAAACTGATTTTT  >  W3110S.gb/3824459‑3824588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: