Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,867,919 C→T 14.6% G73D (GGC→GAC)  mtlA ← fused mannitol‑specific PTS enzyme IIABC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,867,9190CT14.6% 126.6 / 12.4 48G73D (GGC→GAC) mtlAfused mannitol‑specific PTS enzyme IIABC components
Reads supporting (aligned to +/- strand):  ref base C (25/16);  new base T (3/4);  total (28/20)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGATCATCGGCCCGACCAGCT  >  W3110S.gb/3867851‑3867988
                                                                    |                                                                     
tCGGCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCgccgcc                                                                      >  1:2765641/1‑70 (MQ=255)
tCGGCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGTCt                                                                     <  1:3979499/71‑1 (MQ=255)
tCGGCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGTCt                                                                     <  1:3106881/71‑1 (MQ=255)
tCGGCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGTCt                                                                     <  1:2397246/71‑1 (MQ=255)
tCGGCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGTCt                                                                     <  1:2258406/71‑1 (MQ=255)
 cGGCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTa                                                                    >  1:1222969/1‑71 (MQ=255)
 cGGCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTa                                                                    >  1:561111/1‑71 (MQ=255)
 cGGCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTa                                                                    >  1:2208021/1‑71 (MQ=255)
 cGGCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTa                                                                    >  1:1801972/1‑71 (MQ=255)
  ggCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTAc                                                                   >  1:2046831/1‑71 (MQ=255)
  ggCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTAc                                                                   >  1:2833114/1‑71 (MQ=255)
     aTGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGTCTACCAg                                                                >  1:1749823/1‑71 (MQ=255)
        tCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCt                                                              >  1:39293/1‑70 (MQ=255)
        tCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCt                                                              >  1:2872459/1‑70 (MQ=255)
        tCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCt                                                              >  1:3167163/1‑70 (MQ=255)
          tGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTa                                                            >  1:356481/1‑70 (MQ=255)
                 acgaTAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGC‑‑CCTACCAGCTTACCACCGGtat                                                  <  1:3530790/71‑1 (MQ=255)
                   gaTAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCt                                                              >  1:1139675/1‑59 (MQ=255)
                   gaTAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGtat                                                  <  1:4043259/71‑1 (MQ=255)
                   gaTAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGtat                                                  <  1:2460822/71‑1 (MQ=255)
                   gaTAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGtat                                                  <  1:659898/71‑1 (MQ=255)
                   gaTAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGtat                                                  <  1:687538/71‑1 (MQ=255)
                   gaTAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGtat                                                  <  1:3118638/71‑1 (MQ=255)
                    aTAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGt                                                    <  1:3894113/68‑1 (MQ=255)
                      aaCGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAAc                                               >  1:2026816/1‑71 (MQ=255)
                        cGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCg                                             <  1:2025415/71‑1 (MQ=255)
                        cGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCg                                             <  1:3364119/71‑1 (MQ=255)
                        cGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCg                                             <  1:1499441/71‑1 (MQ=255)
                        cGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCg                                             <  1:1494735/71‑1 (MQ=255)
                           ccATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATc                                          <  1:21439/71‑1 (MQ=255)
                               ggtggtGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTac                                                           >  1:337699/1‑50 (MQ=255)
                               ggtggtGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTac                                                           >  1:1327800/1‑50 (MQ=255)
                                gtggtgATGGCACCGACTACGCCGCAACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATcagcag                                     >  1:3740218/1‑71 (MQ=255)
                                      aTGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAg                               >  1:2871976/1‑71 (MQ=255)
                                      aTGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAg                               >  1:691968/1‑71 (MQ=255)
                                             cGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGAt                                           <  1:4057168/52‑1 (MQ=255)
                                              gACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGAt                                           >  1:3140556/1‑51 (MQ=255)
                                              gACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGAt                                           >  1:274569/1‑51 (MQ=255)
                                              gACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGAt                                           >  1:758574/1‑51 (MQ=255)
                                               aCTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATcag                                        >  1:3373400/1‑53 (MQ=255)
                                               aCTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATcag                                        >  1:1621502/1‑53 (MQ=255)
                                                cTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTg                     <  1:2617587/71‑1 (MQ=255)
                                                  aCGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGa                    >  1:980953/1‑70 (MQ=255)
                                                   cgccgcCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGat                   <  1:2334848/70‑1 (MQ=255)
                                                   cgccgcCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGat                   <  1:3649735/70‑1 (MQ=255)
                                                          aCGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGATCATCGGcc           >  1:542628/1‑71 (MQ=255)
                                                            gTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGATCATCGGccc          >  1:1629748/1‑70 (MQ=255)
                                                             ttCGCCGTCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGATCATCGGCCCGa        >  1:399286/1‑71 (MQ=255)
                                                             ttCGCCGTCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGATCATCGGCCCGa        >  1:2044625/1‑71 (MQ=255)
                                                                  cgccTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGATCATCGGCCCGACCAGc   <  1:9593/71‑1 (MQ=255)
                                                                  cgccTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGATCATCGGCCCGACCAGc   <  1:3611903/71‑1 (MQ=255)
                                                                   gccTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGATCATCGGCCCGACCAGCt  >  1:2239180/1‑71 (MQ=255)
                                                                    |                                                                     
TCGGCATGTCTGCGCCGACGATAACGCCCATGGTGGTGATGGCACCGACTACGCCGCCACGTTCGCCGCCTACCAGCTTACCACCGGTATAACCGATCAGCAGCGGCAGGAGATAAGTGATCATCGGCCCGACCAGCT  >  W3110S.gb/3867851‑3867988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: