| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,903,549 | T→C | 19.1% | S104P (TCA→CCA) | bax → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,903,549 | 0 | T | C | 19.1% | 147.8 / 30.9 | 68 | S104P (TCA→CCA) | bax | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (39/16); new base C (6/7); total (45/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
TCTCCGGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTACA > W3110S.gb/3903479‑3903618 | tctcCGGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCAttt > 1:3098023/1‑71 (MQ=255)tctcCGGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCAttt > 1:2219501/1‑71 (MQ=255) cGGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCaa < 1:3058719/70‑1 (MQ=255) cGGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTCCaa < 1:2396864/70‑1 (MQ=255) tAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTAt < 1:3115036/71‑1 (MQ=255) tAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTCCAAAACAGt < 1:1319605/69‑1 (MQ=255) tAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTCCAAAACAGt < 1:1408987/69‑1 (MQ=255) aTGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTAt > 1:2276262/1‑69 (MQ=255) aTGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTAt > 1:3767785/1‑69 (MQ=255) aTGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTAt > 1:1868882/1‑69 (MQ=255) aTGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTAt > 1:1332092/1‑69 (MQ=255) aTGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGATCATTTCAAAACAGTAt > 1:2035323/1‑69 (MQ=255) aCATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGcc < 1:2031925/69‑1 (MQ=255) aCATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGcc < 1:210124/69‑1 (MQ=255) aCATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTCCAAAACa > 1:2672573/1‑58 (MQ=255) aCATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTCCAAAACAGTATCAGGGCCaa > 1:343497/1‑71 (MQ=255) aTTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATg < 1:2443806/71‑1 (MQ=255) tACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCaaaa > 1:2027490/1‑52 (MQ=255) tACCAGCCAAAATGCGGCAATTACTGCGGAACGTAACTGGCTCATTTCaaaa > 1:2001283/1‑52 (MQ=255) tACCAGACAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCaaaa > 1:7449/1‑52 (MQ=255) aCCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATgg > 1:871225/1‑69 (MQ=255) aCCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGt > 1:3927302/1‑70 (MQ=255) aCCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGt > 1:3494579/1‑70 (MQ=255) aCCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGt > 1:434957/1‑70 (MQ=255) gCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTAt < 1:91507/53‑1 (MQ=255) cAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACctgc > 1:214949/1‑71 (MQ=255) cAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACctgc > 1:2035842/1‑71 (MQ=255) aaaTGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGt < 1:3104113/47‑1 (MQ=255) aaaTGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTa < 1:3322301/48‑1 (MQ=255) aaaTGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTa < 1:4074723/48‑1 (MQ=255) aaTGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATc > 1:268754/1‑49 (MQ=255) aaTGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGcc > 1:448824/1‑55 (MQ=255) tGCGGCCATTACTGCGGAACGTAACTGGCTCATTCCaaa < 1:3662414/39‑1 (MQ=255) cGGCCATTACTGCGGAACGTAACTGGCTCATTTCaaa > 1:3387082/1‑37 (MQ=255) cGGCCATTACTGCGGAACGTAACTGGCTCATTTCaaa > 1:1924406/1‑37 (MQ=255) cGGCCATTACTGCGGAACGTAACTGGCTCATTTCaaa > 1:2119038/1‑37 (MQ=255) cGGCCATTACTGCGGAACGTAACTGGCTCATTTCaaa > 1:846903/1‑37 (MQ=255) cGGCCATTACTGCGGAACGTAACTGGCTCATTTCaaa > 1:1832955/1‑37 (MQ=255) cGGCCATTACTGCGGAACGTAACTGGCTCATTTCaaa > 1:3936981/1‑37 (MQ=255) aTTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTc < 1:2159874/53‑1 (MQ=255) ttACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGt < 1:1366301/65‑1 (MQ=255) ttACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTg < 1:3333971/66‑1 (MQ=255) ttACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTg < 1:2583260/66‑1 (MQ=255) ttACTGCGGAACGTAACTGGCTCATTCCAAAACAGt < 1:117099/36‑1 (MQ=255) ttACTGCGGAACGTAACTGGCTCATTCCAAAACAGt < 1:2421122/36‑1 (MQ=255) tACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCAc > 1:2455898/1‑53 (MQ=255) tACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCAc > 1:1378113/1‑53 (MQ=255) tACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCAc > 1:3695777/1‑53 (MQ=255) tACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCAc > 1:61641/1‑53 (MQ=255) aCTGCGGAACGTAACTGGCTCGTTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCt > 1:76663/1‑71 (MQ=255) aCTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCt > 1:438803/1‑71 (MQ=255) aCTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCt > 1:699831/1‑71 (MQ=255) aCTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCt > 1:3635048/1‑71 (MQ=255) aCTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCt > 1:1571921/1‑71 (MQ=255) aCTGCGGAACGTAACTGGCTCATTCCAAAACAGTAt > 1:662085/1‑36 (MQ=255) aCTGCGGAACGTAACTGGCTCATTCCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTc > 1:3348728/1‑70 (MQ=255) tGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTg > 1:1034523/1‑70 (MQ=255) cGGAACGTAACTGGCTCATTCCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGa < 1:692559/69‑1 (MQ=255) aaCGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGAc < 1:2545154/71‑1 (MQ=255) aaCGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGAc < 1:2519412/71‑1 (MQ=255) cGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGaaa > 1:1229064/1‑66 (MQ=255) cGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGaa > 1:735337/1‑65 (MQ=255) aCTGGCTCATTCCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGc > 1:775411/1‑70 (MQ=255) cTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCa < 1:1949717/71‑1 (MQ=255) ggCTCATTCCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGt > 1:654642/1‑53 (MQ=255) cATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCTTGCGCGTCTGAAAGa > 1:766494/1‑57 (MQ=255) cATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGa > 1:3438211/1‑57 (MQ=255) cATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGa > 1:1457784/1‑57 (MQ=255) aTTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGGGTCTGAAAGACATCGCCaa > 1:2591958/1‑65 (MQ=255) aTTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCaa > 1:1449504/1‑65 (MQ=255) tttCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGAc < 1:972815/56‑1 (MQ=255) tttCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGAc < 1:1888064/56‑1 (MQ=255) tttCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGAc < 1:1518110/56‑1 (MQ=255) tttCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTAc < 1:2867058/71‑1 (MQ=255) tttCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTAc < 1:1710998/71‑1 (MQ=255) tttCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTAc < 1:899150/71‑1 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTAc > 1:378282/1‑69 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTAc > 1:306279/1‑69 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTAc > 1:773278/1‑69 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTAc > 1:284094/1‑69 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTAc > 1:1301992/1‑69 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTACa < 1:626429/70‑1 (MQ=255) tCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTACa < 1:554136/70‑1 (MQ=255) | TCTCCGGACCGTAATGCCTTACATTACCAGCCAAAATGCGGCCATTACTGCGGAACGTAACTGGCTCATTTCAAAACAGTATCAGGGCCAATGGTCACCTGCTGAGCGTGCGCGTCTGAAAGACATCGCCAAACGCTACA > W3110S.gb/3903479‑3903618 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |