| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,000,806 | A→T | 25.1% | D37V (GAT→GTT) | yhiO → | predicted universal stress (ethanol tolerance) protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,000,806 | 0 | A | T | 25.1% | 192.9 / 60.7 | 99 | D37V (GAT→GTT) | yhiO | predicted universal stress (ethanol tolerance) protein B |
| Reads supporting (aligned to +/- strand): ref base A (36/38); new base T (11/14); total (47/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 103 total aligned reads displayed.
TGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAGG > W3110S.gb/4000739‑4000874 | tGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc > 1:617843/1‑70 (MQ=255) ttGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:879957/66‑1 (MQ=255) ttGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:1815977/66‑1 (MQ=255) ttGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:3765372/66‑1 (MQ=255) ttGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTg < 1:1305665/71‑1 (MQ=255) ttGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTg < 1:3984268/71‑1 (MQ=255) tGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:2705401/65‑1 (MQ=255) gTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGc < 1:1348249/70‑1 (MQ=255) aCATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTa < 1:193768/70‑1 (MQ=255) aCATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTa < 1:3636135/70‑1 (MQ=255) cATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:2702977/59‑1 (MQ=255) cATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:2043857/59‑1 (MQ=255) cATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:3933474/59‑1 (MQ=255) tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:942895/71‑1 (MQ=255) tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:2240848/71‑1 (MQ=255) tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:1586883/71‑1 (MQ=255) tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:938011/71‑1 (MQ=255) tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:522099/71‑1 (MQ=255) cgcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGt < 1:3173084/71‑1 (MQ=255) gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:4027586/71‑1 (MQ=255) gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:2652661/71‑1 (MQ=255) gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:870938/71‑1 (MQ=255) gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:3009816/71‑1 (MQ=255) gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:3388663/71‑1 (MQ=255) gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:3482692/71‑1 (MQ=255) gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:3678658/71‑1 (MQ=255) gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:1797994/71‑1 (MQ=255) gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:3861725/71‑1 (MQ=255) aTTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAata > 1:483458/1‑64 (MQ=255) aTTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAata > 1:2019243/1‑64 (MQ=255) aTTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAata > 1:1522126/1‑64 (MQ=255) aTTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAata > 1:2442705/1‑64 (MQ=255) tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:1206434/47‑1 (MQ=255) tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa > 1:2355887/1‑61 (MQ=255) tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCa > 1:1312743/1‑60 (MQ=255) tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGAAACAGCGATCCATTGCTGTATCaa > 1:2956751/1‑61 (MQ=255) tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATgg < 1:570792/70‑1 (MQ=255) tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATg > 1:517364/1‑69 (MQ=255) tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAata > 1:161500/1‑61 (MQ=255) tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAata > 1:796744/1‑61 (MQ=255) tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATg > 1:810412/1‑69 (MQ=255) tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATg > 1:254284/1‑69 (MQ=255) tctcATCACTACGCGCGTTGTTAGTGGTACTGAGTAACTGCGATCCa > 1:2508717/1‑47 (MQ=255) tctcATAACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATGCATTGCTCTATCAATATGTTGa > 1:120761/1‑67 (MQ=255) tctaATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAata > 1:2924890/1‑61 (MQ=255) aCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAatat > 1:3070683/1‑55 (MQ=255) aCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCt > 1:265829/1‑71 (MQ=255) aCTACGCGCGTTGTTAGTGCTACTGCGTAACTTCGATCAATTGCTCTATCAATATGTTGATGGAGGGGGCt > 1:1908694/1‑71 (MQ=255) cTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCtt < 1:2399521/71‑1 (MQ=255) cgcgcgTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGa > 1:679244/1‑56 (MQ=255) cgcgcgTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCttttt < 1:2845652/71‑1 (MQ=255) gcgcgTTGTTAGTGGTACTGCTTAACTGCGTTCCATTGCTCTATCAATATCTTGATgg < 1:1085005/58‑1 (MQ=255) gcgcgTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATGGAGTGGGCtttttt < 1:82225/71‑1 (MQ=255) cgTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTAcc > 1:1880675/1‑71 (MQ=255) ttgttAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGtt < 1:1277415/48‑1 (MQ=255) ttgttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATc < 1:719088/40‑1 (MQ=255) ttgttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:2586694/42‑1 (MQ=255) ttgttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:3935691/42‑1 (MQ=255) gttAGTGGTACTGGGTAACTGCGATCCATTGCTCTATCaa > 1:406734/1‑40 (MQ=255) gttAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATTTGTTGATGGAGGGGGCTTTTTTACCTca < 1:2068920/70‑1 (MQ=255) gttAGTGGTACTGCGTAACTGCGATCCATTGCTCTa > 1:885548/1‑36 (MQ=255) aGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGa > 1:2518884/1‑49 (MQ=255) aGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGa > 1:438944/1‑49 (MQ=255) gTACTGCGTAACTGCGTTCCATTGCTCTATCAata > 1:1889268/1‑35 (MQ=255) gTACTGCGTAACTGCGATCCATTGCTCTATCAATatgatg > 1:3092303/1‑40 (MQ=255) gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:63522/39‑1 (MQ=255) gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa > 1:442966/1‑41 (MQ=255) gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa > 1:517129/1‑41 (MQ=255) gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa > 1:1485983/1‑41 (MQ=255) gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa > 1:3863668/1‑41 (MQ=255) gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa > 1:1849945/1‑41 (MQ=255) gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa > 1:1906453/1‑41 (MQ=255) gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa > 1:3161903/1‑41 (MQ=255) tACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:1522073/38‑1 (MQ=255) tACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:280762/38‑1 (MQ=255) aCTCCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTc < 1:518029/60‑1 (MQ=255) tGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATGGa < 1:1620821/41‑1 (MQ=255) tGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATGGa < 1:3342013/41‑1 (MQ=255) tGCGTAACTCCGTTCCATTGCTCTATCAATATGTTGATGGa < 1:473505/41‑1 (MQ=38) gCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATGGAgg > 1:1884274/1‑42 (MQ=255) gCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGc > 1:2920148/1‑64 (MQ=255) gCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGTGGCTTTTTTACCTCACATGGccaaccc > 1:2716216/1‑70 (MQ=255) cGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGccaaccc < 1:3302194/69‑1 (MQ=255) cGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGccaaccc < 1:3149042/69‑1 (MQ=255) cGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACATCACAAGGccaacccaa > 1:1068040/1‑71 (MQ=255) aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCcaacaa < 1:1536399/70‑1 (MQ=255) aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCaacaaa > 1:365210/1‑71 (MQ=255) aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCaacaaa > 1:3023654/1‑71 (MQ=255) aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCaacaaa > 1:1005685/1‑71 (MQ=255) cTGCGTTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca < 1:643502/52‑1 (MQ=255) cTGCGTTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca < 1:3952765/52‑1 (MQ=255) cTGCGTTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca < 1:3801662/52‑1 (MQ=255) gCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCt > 1:165484/1‑48 (MQ=255) gCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCt > 1:2781186/1‑48 (MQ=255) gCGATCCATTGCTCTATCAATATGTTGATGGAGCGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAg > 1:3489323/1‑71 (MQ=255) cGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAgg > 1:1446070/1‑71 (MQ=255) cGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAgg > 1:302611/1‑71 (MQ=255) aTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca < 1:1581116/47‑1 (MQ=255) aTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca < 1:733633/47‑1 (MQ=255) aTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca < 1:1909013/47‑1 (MQ=255) | TGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAGG > W3110S.gb/4000739‑4000874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |