| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,046,323 | G→A | 46.5% | G38D (GGC→GAC) | livG → | leucine/isoleucine/valine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,046,323 | 0 | G | A | 46.5% | 8.0 / 56.6 | 43 | G38D (GGC→GAC) | livG | leucine/isoleucine/valine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (7/16); new base A (12/8); total (19/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01 | |||||||||||
GGCCTGCTGGCGGTGAACAACGTCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAACCACGGTTTTTAACTGTCTGACCGGATTCTACAAACCCACC > W3110S.gb/4046259‑4046384 | ggcCTGCTGGCGGTGAACAACGTCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTaa < 1:2131217/71‑1 (MQ=255) gcCTGCTGGCGGTGAACAACGTCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTaa < 1:3952231/70‑1 (MQ=255) ctgctgGCGGTGAACAACGTCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCt < 1:1482255/66‑1 (MQ=255) ctgctgGCGGTGAACAACGTCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTaa > 1:3479878/1‑68 (MQ=255) ggTGAACAACGTCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGACCCTAACGGTGCCgg > 1:1249365/1‑69 (MQ=255) gTGAACAACGTCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGACCCTAACGGTGCCGGaaa < 1:2293734/71‑1 (MQ=255) gTGAACAACGTCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGACCCTAACGGTGCCGGaaa < 1:2056839/71‑1 (MQ=255) gAACAACGTCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGACCCTAACGGTGCCGGaaaaa > 1:2578873/1‑71 (MQ=255) caacGTCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGACCCTaa > 1:3457890/1‑54 (MQ=255) caacGTCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGACCCTAACGGTGCCGGAAAAACCa > 1:3037387/1‑71 (MQ=255) caacGTCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGACCCTAACGGTGCCGGAAAAACCa > 1:2924482/1‑71 (MQ=255) aacGTCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc < 1:1785982/68‑1 (MQ=255) aacGTCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAAc < 1:1926956/68‑1 (MQ=255) tCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGACCTAAc > 1:3435095/1‑50 (MQ=255) tCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGACCCTAAc < 1:3513726/50‑1 (MQ=255) tCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGACCCTAAc < 1:2652634/50‑1 (MQ=255) aaTCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGACCCTAACGGTGCCGGAAAAACCACGGtttt > 1:1849013/1‑71 (MQ=255) aTCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAACCACGGttttt < 1:155644/71‑1 (MQ=255) ttGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTAACGGTGCCGGaaaa < 1:2127343/56‑1 (MQ=255) ttGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTAACGGTGCCGGaaa < 1:778928/55‑1 (MQ=255) ttGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTAACGGTGCCGGaaa < 1:2550317/55‑1 (MQ=255) ttGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGACCTAACGGTGCCGGaaa < 1:2562402/55‑1 (MQ=255) ttGAACTGTACCCGCAGGAGATCGACTCGTTAATCGGCCCTAACGGTGCCGGAAAAACCACGGTTTTTaa > 1:1177538/1‑70 (MQ=255) gAACTGTACCCGCAGGAGATCGTCTCGTTAATCGACCCTAACGGTGCCGGAAAAACCACGGTTTTTa < 1:1309788/67‑1 (MQ=255) cTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCt < 1:2115228/36‑1 (MQ=255) cTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCt < 1:540220/36‑1 (MQ=255) cTGTACCCGCAGGAGATCGTCTCGTTAATCGACCCt < 1:3673674/36‑1 (MQ=255) tGTACCCGCAGGAGATCGTCTCGTTAATCGACCCTAACGGTGCCGGAAAAACCACGGTTTTTAACTGTc > 1:2871212/1‑69 (MQ=255) gTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTaa > 1:2416125/1‑36 (MQ=255) gTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTaa > 1:3925625/1‑36 (MQ=255) gTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTaa > 1:3517036/1‑36 (MQ=255) cccGCAGGAGATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAACCACGGTTTTTAACTGTCTGAcag < 1:3905408/71‑3 (MQ=255) cccGCAGGAGATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAACCACGGTTTTTAACTGTCTGACCg < 1:2656609/71‑1 (MQ=255) aGGAGATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAACCACGGTTTTTAACTGTCTGACCGGATTc < 1:1298018/71‑1 (MQ=255) gaTCGTCTCGTTAATCGACCCTAACGGTGCCGGaaaaa > 1:1152106/1‑38 (MQ=255) gaTCGTCTCGTTAATCGACCCTAACGGTGCCGGAAAAACCa > 1:2971139/1‑41 (MQ=255) gaTCGTCTCGTTAATCGACCCTAACGGTGCCGGAAAAACCa > 1:1919024/1‑41 (MQ=255) gaTCGTCTCGTTAATCGACCCTAACGGTGCCGGAAAAACCa > 1:3964600/1‑41 (MQ=255) aTCGTCTCGTTAATCGACCCTAACGGTGCCGGAAAAACCACGGTTTTTAACTGTCTGACCGGATTCTACaa < 1:3503700/71‑1 (MQ=255) tCGTCTCGTTAATCGACCCTAACGGTGCCGGAAAAACCACGGTTTTTAACTGTCTGACCGGATTCTACaa < 1:930828/70‑1 (MQ=255) tctcGTTAATCGGCCCTAACGGTGCCGGAAAAACCACGGTTTTTAACTGTCTGACCGGATTCTACAAAccc < 1:1839596/71‑1 (MQ=255) gTTAATCGGCCCTAACGGTGCCGGAAAAACCACGGTTTTTAACTGTCTGACCGGATTCTACAAACCCAcc > 1:1005348/1‑70 (MQ=255) aaTCGACCCTAACGGTGCCGGAAAAACCACGGTTTTTAAc > 1:483402/1‑40 (MQ=255) | GGCCTGCTGGCGGTGAACAACGTCAATCTTGAACTGTACCCGCAGGAGATCGTCTCGTTAATCGGCCCTAACGGTGCCGGAAAAACCACGGTTTTTAACTGTCTGACCGGATTCTACAAACCCACC > W3110S.gb/4046259‑4046384 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |