| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 330,966 | T→G | 13.7% | intergenic (+246/‑629) | betT → / → yahA | choline transporter of high affinity/predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 330,966 | 0 | T | G | 13.7% | 228.2 / 47.9 | 118 | intergenic (+246/‑629) | betT/yahA | choline transporter of high affinity/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (47/54); new base G (9/7); total (57/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
TTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCTGTGTGTGATA > W3110S.gb/330896‑331035 | ttCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAt > 1:85694/1‑71 (MQ=255)ttCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAt > 1:433033/1‑71 (MQ=255) tCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAtg > 1:971670/1‑71 (MQ=255) tCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAtg > 1:2812325/1‑71 (MQ=255) cAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAtgt > 1:2349152/1‑71 (MQ=255) cAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAtgt > 1:3104822/1‑71 (MQ=255) aTAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATgtgt < 1:242761/71‑1 (MQ=255) aTAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATgtgt < 1:1663673/71‑1 (MQ=255) tAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTa < 1:1395591/70‑1 (MQ=255) tAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTa > 1:2244711/1‑70 (MQ=255) tAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTa > 1:333761/1‑70 (MQ=255) tCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATc < 1:41429/71‑1 (MQ=255) tCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATc < 1:3855697/71‑1 (MQ=255) tCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATc < 1:557967/71‑1 (MQ=255) ttATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCaa > 1:587433/1‑71 (MQ=255) ttATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCaa > 1:868787/1‑71 (MQ=255) ttATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCaa > 1:1194460/1‑71 (MQ=255) tAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGa < 1:1372542/70‑1 (MQ=255) tAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGa < 1:2612025/70‑1 (MQ=255) tAATGTAAATATTGATGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGa < 1:2160953/70‑1 (MQ=255) tGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAAtt < 1:3624943/71‑1 (MQ=255) tAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTaaa > 1:145102/1‑57 (MQ=255) tAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTaa > 1:2351321/1‑71 (MQ=255) tAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTa < 1:2863459/70‑1 (MQ=255) tAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTa < 1:709268/70‑1 (MQ=255) tAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTa < 1:2104034/70‑1 (MQ=255) tAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTa < 1:2023011/70‑1 (MQ=255) tAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTaa < 1:423716/71‑1 (MQ=255) tAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTaa > 1:3912421/1‑71 (MQ=255) aaTATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACg > 1:3801557/1‑71 (MQ=255) aaTATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACg > 1:1006360/1‑71 (MQ=255) aaTATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACg > 1:3620562/1‑71 (MQ=255) aaTATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACg > 1:3909480/1‑71 (MQ=255) atTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCaa > 1:3075767/1‑71 (MQ=255) ttGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTaa > 1:3919703/1‑50 (MQ=255) ttGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAAc > 1:1132823/1‑56 (MQ=255) ttGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGa < 1:1735329/59‑1 (MQ=255) gCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCa > 1:861268/1‑71 (MQ=255) gCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCa > 1:2599266/1‑71 (MQ=255) gCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCa > 1:4038733/1‑71 (MQ=255) tGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCa < 1:2816067/65‑1 (MQ=255) aTCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTg < 1:1642698/52‑1 (MQ=255) aTCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCa < 1:362205/63‑1 (MQ=255) aTCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCa < 1:561340/63‑1 (MQ=255) aTCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCAtt < 1:3452930/69‑1 (MQ=255) tCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCa < 1:2759003/62‑1 (MQ=255) tCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTg < 1:2036259/70‑1 (MQ=255) cATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGt > 1:3802838/1‑70 (MQ=255) cATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGt > 1:956225/1‑70 (MQ=255) cATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGt > 1:1398715/1‑70 (MQ=255) aTTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTa > 1:1732220/1‑71 (MQ=255) aTTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTa > 1:64650/1‑71 (MQ=255) aTTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTa > 1:3941343/1‑71 (MQ=255) tGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTAtt > 1:1249206/1‑70 (MQ=255) aaaTGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTa < 1:1770101/70‑1 (MQ=255) aCGCATTATTCATGAGAAATGTGTATCGTAAATCaa > 1:2214657/1‑36 (MQ=255) aCGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTAtt > 1:789860/1‑63 (MQ=255) aCGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGtt > 1:1789894/1‑70 (MQ=255) aCGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGtt > 1:1445888/1‑70 (MQ=255) aCGCATTATTCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGtt > 1:2813042/1‑70 (MQ=255) cGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTa < 1:2515125/71‑1 (MQ=255) cGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTa < 1:2376186/71‑1 (MQ=255) ttattCATGAGAAATGTGTATCGTAAATCAACTGAAAt < 1:3081096/38‑1 (MQ=255) ttattCATGAGAAATGTGTATCGTAAATCAACTGAAAt < 1:1374861/38‑1 (MQ=255) ttattCATGAGAAATGTGTATCGTAAATCAACTGAAAt < 1:1980298/38‑1 (MQ=255) ttattCATGAGAAATGTGTATCGTAAATCAACTGAAAt < 1:2849579/38‑1 (MQ=255) ttattCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGc < 1:3503782/44‑1 (MQ=255) ttattCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTa > 1:1589764/1‑71 (MQ=255) tattCATGAGAAATGTGTATCGTAAATCAACTGAAAtt < 1:3875666/38‑1 (MQ=255) attGATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAgg > 1:522085/1‑61 (MQ=255) attCATGAGAAATGTGTATCGTAAATCAACTGAAATTAAc > 1:3577638/1‑40 (MQ=255) attCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAgg > 1:2185289/1‑61 (MQ=255) attCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAgg > 1:578817/1‑61 (MQ=255) attCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAgg > 1:488104/1‑61 (MQ=255) attCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAg > 1:3316487/1‑60 (MQ=255) attCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAg > 1:3016189/1‑60 (MQ=255) attCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAg > 1:2754997/1‑60 (MQ=255) attCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAg > 1:2510858/1‑60 (MQ=255) attCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATc > 1:2228051/1‑71 (MQ=255) attCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATc > 1:625020/1‑71 (MQ=255) attCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATc > 1:2765603/1‑71 (MQ=255) attCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATc > 1:943356/1‑71 (MQ=255) ttCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCt > 1:2474517/1‑71 (MQ=255) ttCATGAGAAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCt > 1:2583071/1‑71 (MQ=255) cATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtgt > 1:740393/1‑71 (MQ=255) cATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtg < 1:835315/70‑1 (MQ=255) aTGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtgt < 1:3633758/70‑1 (MQ=255) gagaAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtgtgtg > 1:4064592/1‑71 (MQ=255) agaAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGt > 1:1540419/1‑55 (MQ=255) agaAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGt > 1:1963599/1‑55 (MQ=255) agaAAGGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtgtgtgt < 1:1049834/71‑1 (MQ=255) gaAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCtgtgtgtg < 1:3313972/71‑1 (MQ=255) aTGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGttt < 1:2460384/52‑1 (MQ=255) aTGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGtt < 1:1022881/52‑1 (MQ=255) aTGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGtt < 1:886186/52‑1 (MQ=255) aTGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGtt < 1:1988220/52‑1 (MQ=255) aTGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGtt < 1:2871289/52‑1 (MQ=255) aTGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCTGTGTGTGata < 1:2429249/71‑1 (MQ=255) tgtgTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGttt < 1:3281545/52‑1 (MQ=255) tgtgTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGttt < 1:618885/52‑1 (MQ=255) | TTCAATAAGTTTAGTCTTATTTAATGTAAATATTGCTGATCATTTGAAATGACGCATTATTCATGAGAAATGTGTATCGTAAATCAACTGAAATTAACGCAACCATTTGTTATTTAAGGTTTAATTATCTGTGTGTGATA > W3110S.gb/330896‑331035 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |