| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,068,392 | T→C | 33.3% | M493T (ATG→ACG) | glgB → | 1,4‑alpha‑glucan branching enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,068,392 | 0 | T | C | 33.3% | 110.0 / 92.5 | 96 | M493T (ATG→ACG) | glgB | 1,4‑alpha‑glucan branching enzyme |
| Reads supporting (aligned to +/- strand): ref base T (24/40); new base C (17/15); total (41/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
GGCGGTCTGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTACA > W3110S.gb/4068331‑4068460 | ggCGGTCTGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGa > 1:2949904/1‑71 (MQ=255)ggCGGTCTGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGa > 1:76208/1‑71 (MQ=255) ttggCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGtt > 1:2446861/3‑71 (MQ=255) ggCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGttt < 1:3338305/70‑1 (MQ=255) cTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATc < 1:2218585/71‑1 (MQ=255) cTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCGCGACCCGGTTTATc < 1:3009424/71‑1 (MQ=255) cTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTTGACTACATGAAGCTCGACCCGGTTTATCg < 1:1070809/69‑1 (MQ=255) cTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACAt > 1:378959/1‑48 (MQ=255) cTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCg < 1:1154194/69‑1 (MQ=255) cTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGt > 1:2530919/1‑70 (MQ=255) cTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGt > 1:640164/1‑70 (MQ=255) cAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTat > 1:738328/1‑70 (MQ=255) cAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTat > 1:1406156/1‑70 (MQ=255) cAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTat > 1:266597/1‑70 (MQ=255) aaGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTatca > 1:127406/1‑71 (MQ=255) aGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTatcat < 1:317250/71‑1 (MQ=255) tGGAACCTCGGCTGTATGCATGACACCCTGGACTACATGAAGCTCGACCCGGttt < 1:599608/55‑1 (MQ=255) tGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTa < 1:173750/65‑1 (MQ=255) tGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTAtcatca > 1:920619/1‑71 (MQ=255) tGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTAtcatca > 1:2705682/1‑71 (MQ=255) tGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTAtcatca > 1:1698153/1‑71 (MQ=255) tGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTAtaatca > 1:842509/1‑71 (MQ=255) tGGAACCTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGCCCCGGTTTATCGTCAGTAtcatca > 1:494947/1‑71 (MQ=255) tGGAACCTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTAtcatca > 1:1984786/1‑71 (MQ=255) gAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCAc > 1:3515403/1‑70 (MQ=255) gAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCAc < 1:1455600/70‑1 (MQ=255) gAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCAc < 1:3513982/70‑1 (MQ=255) gAACCTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCg > 1:1456295/1‑49 (MQ=255) gAACCTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCAc > 1:3251664/1‑70 (MQ=255) ccTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGttt < 1:1389397/50‑1 (MQ=255) ccTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGttt < 1:4039957/50‑1 (MQ=255) ccTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGttt < 1:341305/50‑1 (MQ=255) ccTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGttt < 1:2173164/50‑1 (MQ=255) ccTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATa < 1:2362457/71‑1 (MQ=255) ccTCGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTATAATCACGAt > 1:3848345/1‑70 (MQ=255) cTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGttt < 1:989724/49‑1 (MQ=255) cTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATaa < 1:3879266/71‑1 (MQ=255) tCGGCTGGATGCATGACACACTGGAGTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATaa > 1:1327243/1‑70 (MQ=255) cGGCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATaa < 1:3333747/69‑1 (MQ=255) gCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTg < 1:1015200/71‑1 (MQ=255) gCTGGATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTg < 1:3989066/71‑1 (MQ=255) tGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTAt < 1:16565/45‑1 (MQ=255) gATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTatcat < 1:2981326/55‑1 (MQ=255) gATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGAt < 1:3574135/61‑1 (MQ=255) gATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGAt < 1:719921/61‑1 (MQ=255) gATGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGAt < 1:4010185/61‑1 (MQ=255) aTGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTat > 1:1620101/1‑51 (MQ=255) aTGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTat > 1:4053771/1‑51 (MQ=255) aTGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTat > 1:3582546/1‑51 (MQ=255) aTGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTa > 1:3669271/1‑50 (MQ=255) aTGCATGACACCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTa > 1:4058205/1‑50 (MQ=255) tGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTat < 1:3753611/50‑1 (MQ=255) tGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTat < 1:2722934/50‑1 (MQ=255) tGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTat < 1:447642/50‑1 (MQ=255) cATGACACCCTGGTCTACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCgg < 1:1658739/71‑1 (MQ=255) cATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATaaa > 1:3341851/1‑60 (MQ=255) cATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATaaa > 1:1903387/1‑60 (MQ=255) aTGACACCCTGGTCTACACGAAGCTCGACCCGGTTTCTCGGCAGTATCATCACGATAAACTGACCTTCgg < 1:3570732/70‑1 (MQ=255) aTGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCggg < 1:4063086/71‑1 (MQ=255) tGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATa < 1:2801425/56‑1 (MQ=255) acacCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGaa > 1:1775171/1‑69 (MQ=255) acacCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGAtt > 1:2025034/1‑71 (MQ=255) acacCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGAtt > 1:2908674/1‑71 (MQ=255) acacCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGAt < 1:1165774/70‑1 (MQ=255) acacCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGAt > 1:2894999/1‑70 (MQ=255) cacCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATtc < 1:3089105/71‑1 (MQ=255) cacCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATtc < 1:1528329/71‑1 (MQ=255) acCCTGGACTACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATtc < 1:2438763/70‑1 (MQ=255) cccTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATtctc > 1:639940/1‑71 (MQ=255) cTGGACTACATGAAGCTCGACCCGGTTTATCGTCAg < 1:898439/36‑1 (MQ=255) cTGGACTACATGAAGCTCGACCCGGTTTATCGTCAg < 1:2922452/36‑1 (MQ=255) cTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCAc < 1:994416/45‑1 (MQ=255) cTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATa < 1:3815882/49‑1 (MQ=255) tGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATtctc < 1:3410114/68‑1 (MQ=255) tGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATtctc < 1:2501108/68‑1 (MQ=255) ggACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATaaa > 1:3854427/1‑49 (MQ=255) ggACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATaaa > 1:2514230/1‑49 (MQ=255) ggACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATaaa > 1:337243/1‑49 (MQ=255) gACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTa > 1:1792623/1‑68 (MQ=255) gACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTAc < 1:1251049/69‑1 (MQ=255) gACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACaa > 1:466480/1‑71 (MQ=255) aCTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATtc < 1:293765/63‑1 (MQ=255) tACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATa < 1:3584893/43‑1 (MQ=255) tACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATa < 1:2429276/43‑1 (MQ=255) tACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATa < 1:3659517/43‑1 (MQ=255) tACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTAc < 1:324244/66‑1 (MQ=255) tACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCAc < 1:439077/39‑1 (MQ=255) tACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCAc < 1:2861769/39‑1 (MQ=255) tACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCAc < 1:2857070/39‑1 (MQ=255) tACACGAAGCTCGACCCGGTTTATCGTCAGTATCATCAc < 1:1041887/39‑1 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTaca > 1:3829723/1‑71 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTaca > 1:844382/1‑71 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTaca > 1:227881/1‑71 (MQ=255) cATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTac > 1:4024887/1‑70 (MQ=255) aTGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTg < 1:564057/45‑1 (MQ=255) aTGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTg < 1:3788957/45‑1 (MQ=255) aTGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTg < 1:3538349/45‑1 (MQ=255) | GGCGGTCTGGGCTTCTGGTACAAGTGGAACCTCGGCTGGATGCATGACACCCTGGACTACATGAAGCTCGACCCGGTTTATCGTCAGTATCATCACGATAAACTGACCTTCGGGATTCTCTACAACTACA > W3110S.gb/4068331‑4068460 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |