Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	4,074,022	G→A	20.4%	A63T (GCC→ACC)	glgP →	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,074,022	0	G	A	20.4%	94.1 / 18.8	44	A63T (GCC→ACC)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (14/21);  new base A (3/6);  total (17/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01

TGGCTGAACGCAACGTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGTTTTTGATTGGCCGTACG  >  W3110S.gb/4073956‑4074087                                                                   |                                                                  tagCTGAACGCAACGTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTAcc                                                                 >  1:196725/3‑69 (MQ=255) tGGCTGAACGCAACGTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTAcc                                                                 >  1:642025/1‑69 (MQ=255) tGGCTGAACGCAACGTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTAcc                                                                 >  1:609314/1‑69 (MQ=255)       aaCGCAACGTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTc                                                         <  1:3805300/71‑1 (MQ=255)         cGCAACGTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCg                                                        >  1:901874/1‑70 (MQ=255)             aCGTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCGCAAGa                                                   <  1:382591/71‑1 (MQ=255)             aCGTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCGCAAGa                                                   <  1:1996747/71‑1 (MQ=255)             aCGTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCGCAAGa                                                   <  1:1988725/71‑1 (MQ=255)             aCGTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCGCAAGa                                                   <  1:78119/71‑1 (MQ=255)             aCGTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTACCCAGTTGTCGCAAGa                                                   <  1:2411586/71‑1 (MQ=255)             aCGTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTACCCAGTTGTCGCAAGa                                                   <  1:2259091/71‑1 (MQ=255)              cGTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCGc                                                       <  1:1611157/66‑1 (MQ=255)               gTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCGCAAGaaa                                                 >  1:2746707/1‑71 (MQ=255)                      gCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTc                                           <  1:3907591/70‑1 (MQ=255)                          tgCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGtt                                      <  1:1295450/71‑1 (MQ=255)                            cgcgATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGttt                                     <  1:1501237/70‑1 (MQ=255)                            cgcgATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGttt                                     <  1:1060289/70‑1 (MQ=255)                            cgcgATCGTCTCGTGGAGCGCTGG‑TACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTtac                                   >  1:1952399/1‑71 (MQ=255)                              cgATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTtact                                  <  1:3960305/71‑1 (MQ=255)                                      tcGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTc                           >  1:1979961/1‑70 (MQ=255)                                        gTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTc                           <  1:2399530/68‑1 (MQ=255)                                        gTGGAGCGCTGGTTACGTTCAAACCGTACCCAGTTCTCGCAAGAAACTCGTCAGGTTTACTACCTGTc                           <  1:153250/68‑1 (MQ=255)                                                  ggTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGttt                 >  1:3805847/1‑68 (MQ=255)                                                   gTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGttttt               <  1:3566588/69‑1 (MQ=255)                                                   gTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGttttt               <  1:3662579/69‑1 (MQ=255)                                                   gTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGttttt               <  1:3584228/69‑1 (MQ=255)                                                   gTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGttttt               <  1:3628573/69‑1 (MQ=255)                                                   gTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGTTTTTg              <  1:1312800/70‑1 (MQ=255)                                                   gTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGTTTTTg              <  1:2810995/70‑1 (MQ=255)                                                   gTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGTTTTTg              <  1:3710174/70‑1 (MQ=255)                                                    ttACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGTTTTTGAt            <  1:1148739/71‑1 (MQ=255)                                                     tACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGTTTTTGAtt           >  1:2369057/1‑71 (MQ=255)                                                      aCGTTCAAACCGTGCCCAGTTGTCGCAAGAAAATCGTCAGGTTTACTACCTGTCGATGGAGTTTTTGATTg          >  1:13080/1‑71 (MQ=255)                                                         ttCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGTTTTTGAtt           >  1:3572298/1‑67 (MQ=255)                                                         ttCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGTTTTTGATTGGcc       >  1:216410/1‑71 (MQ=255)                                                         ttCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGTTTTTGATTGGc        >  1:239873/1‑70 (MQ=255)                                                         ttCAAACCGTACCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATg                       <  1:3384404/55‑1 (MQ=255)                                                         ttCAAACCGTACCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATg                       <  1:1684094/55‑1 (MQ=255)                                                         ttCAAACCGTACCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATg                       <  1:608701/55‑1 (MQ=255)                                                              aCCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGtt                  <  1:2017438/55‑1 (MQ=255)                                                              aCCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGTTTTTGATTGGCCGTGCg  >  1:3486796/1‑71 (MQ=255)                                                              aCCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGTTTTTGATTGGCCGTACg  >  1:2031988/1‑71 (MQ=255)                                                              aCCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGTTTTTGATTGGCCGTACg  >  1:1512869/1‑71 (MQ=255)                                                              aCCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGTTTTTGATTGGCCGTACg  >  1:1146481/1‑71 (MQ=255)                                                                   |                                                                  TGGCTGAACGCAACGTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCAAACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGTTTTTGATTGGCCGTACG  >  W3110S.gb/4073956‑4074087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑