| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,085,470 | G→A | 20.0% | R622C (CGT→TGT) | malT ← | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,085,470 | 0 | G | A | 20.0% | 119.8 / 24.3 | 55 | R622C (CGT→TGT) | malT | DNA‑binding transcriptional activator, maltotriose‑ATP‑binding |
| Reads supporting (aligned to +/- strand): ref base G (25/19); new base A (2/9); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.03e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
TATTTGCCATTCCCCAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGATAAGACGACAA > W3110S.gb/4085403‑4085536 | tatTTGCCATTCCCCAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACAGGc > 1:2367046/1‑71 (MQ=255) tttGCCATTCCCCAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGc > 1:1955306/1‑69 (MQ=255) ccATTCCCCAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCg < 1:902480/70‑1 (MQ=255) ccATTCCCCAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCg < 1:2722377/70‑1 (MQ=255) ccATTCCCCAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCg < 1:2048608/70‑1 (MQ=255) ccATTCCCCAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCg < 1:2043592/70‑1 (MQ=255) ccccAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACAGGCCAGCGAGCAtt > 1:1921744/1‑71 (MQ=255) cccAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACAGGCCAGCGAGCATTg < 1:2362915/71‑1 (MQ=255) agGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCaa < 1:728733/71‑1 (MQ=255) agGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACAGGCCAGCGAGCATTGAATCaa < 1:951396/71‑1 (MQ=255) agGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACAGGCCAGCGAGCATTGAATCaa < 1:3819678/71‑1 (MQ=255) ggTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGa > 1:1625026/1‑65 (MQ=255) gTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACAGGCCAGCGAGCATTGAATCAACa < 1:1062427/71‑1 (MQ=255) ttttCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCAt > 1:773578/1‑60 (MQ=255) ttttCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCAt > 1:3575908/1‑60 (MQ=255) ttttCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCAt > 1:325911/1‑60 (MQ=255) ttCCAGACGGTTCAGCTGGCTACGGGCGTTATATAAATCACCACGGGCCAGCGAGCATTGAATCAACATTg > 1:850526/1‑71 (MQ=255) tCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTg < 1:2138892/59‑1 (MQ=255) gACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAgg > 1:3736619/1‑71 (MQ=255) gACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACAGGCCAGCGAGCATTGAATCAACATTGCCAgg < 1:2644434/71‑1 (MQ=255) tGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTg < 1:3589773/44‑1 (MQ=255) ggCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGt > 1:134234/1‑71 (MQ=255) ggCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGt > 1:722517/1‑71 (MQ=255) gCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGaa > 1:1668959/1‑44 (MQ=255) gCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGaa > 1:3876553/1‑44 (MQ=255) gCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGaa > 1:313569/1‑44 (MQ=255) cTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGtt > 1:598922/1‑70 (MQ=255) cTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTg > 1:144829/1‑71 (MQ=255) cTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTg > 1:3796183/1‑71 (MQ=255) tACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACAt > 1:2528024/1‑49 (MQ=255) aCGGGCGTTATCTAAATCACCACAGGCCAGCTAGCATTGAATCaa < 1:279363/45‑1 (MQ=255) cGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGc < 1:1504747/69‑1 (MQ=255) gggCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCtgt < 1:2587156/71‑1 (MQ=255) gggCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCtgt < 1:4033719/71‑1 (MQ=255) ggCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCtgtg > 1:1315597/1‑71 (MQ=255) ttATCTAAATCACCACGGGCCAGCGAGCATTGAATCaa < 1:3125871/38‑1 (MQ=255) ttATCTAAATCACCACAGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGc < 1:724156/57‑1 (MQ=255) ttATCTAAATCACCACAGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGc < 1:80469/57‑1 (MQ=255) aTCTAAATCACCACGGGCCAGCGAGCATTGAATCAACa > 1:1352948/1‑38 (MQ=255) aTCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGc > 1:2041532/1‑55 (MQ=255) aTCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGa > 1:3432512/1‑70 (MQ=255) tCTAAATCACCACGGGCCAGCGAGCATTGAATCaa < 1:1563010/35‑1 (MQ=255) tCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGtt < 1:3865369/58‑1 (MQ=255) tCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGtt < 1:3915915/58‑1 (MQ=255) aaTCACCACGGGCCAGCGAGCATTGAATCAACATTGc > 1:1414302/1‑37 (MQ=255) aaTCACCACGGGCCAGCGAGCATTGAATCAACATTGc > 1:1562774/1‑37 (MQ=255) aaTCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGATaa < 1:3729832/68‑1 (MQ=255) aTCACCACGGGCCAGCGAGCATTGAATCAACATTGc < 1:3822498/36‑1 (MQ=255) aTCACCACGGGCCAGCGAGCATTGAATCAACATTGc < 1:1374631/36‑1 (MQ=255) tCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGATAAgacga > 1:2548997/1‑71 (MQ=255) accacGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGATAAgacgac > 1:3642147/1‑70 (MQ=255) accacGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGATAAgacgac < 1:1460742/70‑1 (MQ=255) accacGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGATAAgacga > 1:464454/1‑69 (MQ=255) accacAGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGATAAgacgac < 1:1899580/70‑1 (MQ=255) ccacGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGATAAGACGACaa < 1:1108620/71‑1 (MQ=255) | TATTTGCCATTCCCCAGCAGGTTTTCCAGACGGTTCAGCTGGCTACGGGCGTTATCTAAATCACCACGGGCCAGCGAGCATTGAATCAACATTGCCAGGCACTGAAGCTGTTGCTGTGGCTGATAAGACGACAA > W3110S.gb/4085403‑4085536 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |