| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,092,715 | G→A | 33.5% | A382V (GCG→GTG) | gntT ← | gluconate transporter, high‑affinity GNT I system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,092,715 | 0 | G | A | 33.5% | 41.4 / 32.8 | 39 | A382V (GCG→GTG) | gntT | gluconate transporter, high‑affinity GNT I system |
| Reads supporting (aligned to +/- strand): ref base G (12/13); new base A (3/10); total (15/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01 | |||||||||||
AAAGTACTCTTTGAACAGCCAGAAGCCCGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGATACCACCCGCAGTG > W3110S.gb/4092645‑4092783 | aaaGTACTCTTTGAACAGCCAGAAGCCCGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCg > 1:3111967/1‑71 (MQ=255) tttGAACAGCCAGAAGCCCGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCa > 1:3923796/1‑71 (MQ=255) tttGAACAGCCAGAAGCCCGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCa > 1:2905085/1‑71 (MQ=255) ttGAACAGCCAGAAGCCCGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAAc < 1:4080585/68‑1 (MQ=255) aGCCCGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCACAATAACCATCAGCTCCGGGCt < 1:904097/70‑1 (MQ=255) gCCCGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCACAATaa > 1:3588373/1‑53 (MQ=255) ccGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGc < 1:3600405/46‑1 (MQ=255) ccGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGc < 1:31233/46‑1 (MQ=255) ccGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAaca < 1:3020279/71‑1 (MQ=255) cGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGc < 1:367844/45‑1 (MQ=255) aTCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCcg > 1:3191576/1‑71 (MQ=255) tCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCAt > 1:1580406/1‑50 (MQ=255) gTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCcgtcg > 1:2258377/1‑71 (MQ=255) ttCACATGAGAGAAAATCACACTACCGGAACCAACCACAATAACCATCAGCTCCGGGCt < 1:951177/59‑1 (MQ=255) ttCACATGAGAGAAAATCACACTACCGGAACCAACCACAATAACCATCAGCTCCGGGCt < 1:1020949/59‑1 (MQ=255) ttCACATGAGAGAAAATCACACTACCGGAACCAACCACAATAACCATCAGCTCCGGGCTAaca < 1:319442/63‑1 (MQ=255) caTGAGAGAAAATCACACTACCGGAACCAACCTCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCa < 1:3943234/71‑1 (MQ=255) caTGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCa < 1:1380573/71‑1 (MQ=255) caTGAGAGAAAATCACACTACCGGAACCAACCACAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCa < 1:2479932/71‑1 (MQ=255) agagagaAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTaa > 1:2001021/2‑55 (MQ=255) agagaAAATCACACTACCGGAACCAACCGCAATAACCATc > 1:3729156/1‑40 (MQ=255) agaAAATCACACTACCGGAACCAACCACAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGc < 1:228019/71‑1 (MQ=255) tCACACTACCGGAACCAACCACAATAACCATCAGCTCCGGGCt < 1:2012641/43‑1 (MQ=255) tCACACTACCGGAACCAACCACAATAACCATCAGCTCCGGGCt < 1:427018/43‑1 (MQ=255) acacTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCcgc < 1:3171782/71‑1 (MQ=255) acacTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCcgc < 1:1419773/71‑1 (MQ=255) acacTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCcgc < 1:655122/71‑1 (MQ=255) cTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGAt < 1:2454985/71‑1 (MQ=255) cTACCGGAACCAACCACAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGc < 1:2049097/53‑1 (MQ=255) cTACCGGAACCAACCACAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGc < 1:1696099/53‑1 (MQ=255) cTACCGGAACCAACCACAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGa > 1:2166050/1‑70 (MQ=255) aCCGGAACCAACCACAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGATac > 1:1651007/1‑71 (MQ=255) ggAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTg > 1:1200409/1‑59 (MQ=255) ggAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGATaccac > 1:1765763/1‑71 (MQ=255) ggAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGATaccac > 1:843492/1‑71 (MQ=255) ccaaccGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGc > 1:1146656/1‑56 (MQ=255) ccaaccGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGATaccac > 1:3943528/1‑67 (MQ=255) accGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGATACCACCCGCAGt < 1:3751811/71‑1 (MQ=255) ccGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGATACCACCCGCAGTg < 1:3900689/71‑1 (MQ=255) ccGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGATACCACCCGCAGTg < 1:3803592/71‑1 (MQ=255) | AAAGTACTCTTTGAACAGCCAGAAGCCCGGATCGTTCACATGAGAGAAAATCACACTACCGGAACCAACCGCAATAACCATCAGCTCCGGGCTAACACCCGTCGTTGCAATCAGCGGTGCCGCGATACCACCCGCAGTG > W3110S.gb/4092645‑4092783 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |