| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,184,035 | C→A | 25.5% | G3* (GGA→TGA) R269M (AGG→ATG) |
gspD ← gspC ← |
general secretory pathway component, cryptic general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,184,035 | 0 | C | A | 25.5% | 104.7 / 20.8 | 47 | G3* (GGA→TGA) R269M (AGG→ATG) | gspD gspC | general secretory pathway component, cryptic general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base C (18/17); new base A (5/7); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.83e-01 | |||||||||||
AGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCGGGGTACGCC > W3110S.gb/4183973‑4184102 | aGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCAt < 1:1211612/69‑1 (MQ=255)aGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCAt < 1:1782978/69‑1 (MQ=255) tCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGt > 1:2279252/1‑71 (MQ=255) tCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGt > 1:1944551/1‑71 (MQ=255) tCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGt > 1:1738147/1‑71 (MQ=255) cacaAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGa > 1:1156466/1‑60 (MQ=255) cacaAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGa > 1:300221/1‑60 (MQ=255) cacaAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGa > 1:1827548/1‑60 (MQ=255) cacaAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCcatt < 1:3976947/71‑1 (MQ=255) cacaAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcatt < 1:2391140/71‑1 (MQ=255) aaGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCcattcat < 1:2292263/71‑1 (MQ=255) aaGGCATGAATAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCcattcat < 1:3897413/71‑1 (MQ=255) aTGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTg > 1:3805107/1‑71 (MQ=255) aTGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTg > 1:3100809/1‑71 (MQ=255) tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGt < 1:1536296/71‑1 (MQ=255) tGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCAt < 1:549811/49‑1 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATg > 1:2536109/1‑48 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGa > 1:1195419/1‑49 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGa > 1:3045206/1‑49 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGa > 1:2629465/1‑49 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGa > 1:2440149/1‑49 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTa < 1:3039497/70‑1 (MQ=255) ctgcCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTcccc < 1:2031073/71‑1 (MQ=255) ccAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCg < 1:3342109/69‑1 (MQ=255) ccAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCg < 1:2110433/69‑1 (MQ=255) cAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTaa < 1:1600538/60‑1 (MQ=255) agcaagcaGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTaa < 1:106593/59‑1 (MQ=255) agcagGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTccc > 1:3178075/1‑58 (MQ=255) agcagGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACAtt < 1:2232397/71‑1 (MQ=255) cagGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTa < 1:85100/71‑1 (MQ=255) gTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCa < 1:1828918/71‑1 (MQ=255) tGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATc > 1:1362470/1‑69 (MQ=255) tGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATc > 1:2186412/1‑69 (MQ=255) gATTTTATTGAGTCCTTTCATGACGCAGTCcattca > 1:2020238/1‑36 (MQ=255) gATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAg > 1:2815439/1‑46 (MQ=255) gATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAg > 1:3852148/1‑46 (MQ=255) gATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAg > 1:1455929/1‑46 (MQ=255) aTTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGt < 1:1313347/42‑1 (MQ=255) aTTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGt < 1:384628/42‑1 (MQ=255) aTTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGt < 1:1692355/42‑1 (MQ=255) aTTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGt < 1:1173363/42‑1 (MQ=255) aTTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGc > 1:2174016/1‑71 (MQ=255) aTTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGc > 1:2918607/1‑71 (MQ=255) ttATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCACCGAc > 1:2339804/1‑51 (MQ=255) aTTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCgggg < 1:3587646/70‑1 (MQ=255) gTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCGGGGTACGcc < 1:2470577/71‑1 (MQ=255) gTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCGGGGTACGcc < 1:2095031/71‑1 (MQ=255) | AGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCGGGGTACGCC > W3110S.gb/4183973‑4184102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |