| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,208,832 | A→G | 32.9% | H126R (CAC→CGC) | yrdD → | predicted DNA topoisomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,208,832 | 0 | A | G | 32.9% | 84.7 / 70.9 | 76 | H126R (CAC→CGC) | yrdD | predicted DNA topoisomerase |
| Reads supporting (aligned to +/- strand): ref base A (23/28); new base G (13/12); total (36/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01 | |||||||||||
AATTACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGCCCTG > W3110S.gb/4208764‑4208900 | aaTTACAAGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCAc < 1:139509/70‑1 (MQ=255) tACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCActct > 1:3654944/1‑70 (MQ=255) tACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCActct > 1:3723266/1‑70 (MQ=255) tACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCtt > 1:1057638/1‑71 (MQ=255) tACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCtt > 1:3650284/1‑71 (MQ=255) tACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCtt > 1:3793167/1‑71 (MQ=255) tACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCtt > 1:424506/1‑71 (MQ=255) tACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCtt > 1:1280768/1‑71 (MQ=255) tACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCtt > 1:51116/1‑71 (MQ=255) tACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCAGCTCCCGTTATGGCAAAACATTTCActct > 1:3664813/1‑70 (MQ=255) aCATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTtg > 1:1805622/1‑71 (MQ=255) aCATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCActct > 1:3292167/1‑69 (MQ=255) aCATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTtg > 1:401861/1‑71 (MQ=255) aCATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTtg > 1:711997/1‑71 (MQ=255) cATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTtgt < 1:300808/71‑1 (MQ=255) cATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTtgt < 1:4045958/71‑1 (MQ=255) cATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTtgt < 1:3195843/71‑1 (MQ=255) aTGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTtgtg < 1:2674805/71‑1 (MQ=255) aTGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTtgtg < 1:2167162/71‑1 (MQ=255) cccccAATGTCGGAC‑‑GCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGc > 1:485452/1‑71 (MQ=255) cAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCt > 1:220496/1‑70 (MQ=255) cAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTa > 1:1329081/1‑71 (MQ=255) aaTGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTAc < 1:2955126/71‑1 (MQ=255) tGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTtgtg < 1:117730/61‑1 (MQ=255) gTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCg > 1:2371655/1‑71 (MQ=255) gTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCg > 1:3006040/1‑71 (MQ=255) gTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCg > 1:3922335/1‑71 (MQ=255) tCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCgg < 1:2739799/71‑1 (MQ=255) tCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCgg < 1:3440602/71‑1 (MQ=255) tCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCgg < 1:1052516/71‑1 (MQ=255) tCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCgg < 1:3007931/71‑1 (MQ=255) cGGACGGGCCATCTGTTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTtgtg < 1:1473070/58‑1 (MQ=255) ggACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTtgtg < 1:2140191/57‑1 (MQ=255) cGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCt > 1:32287/1‑60 (MQ=255) ggCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCa < 1:1482858/71‑1 (MQ=255) ggCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCa < 1:4043409/71‑1 (MQ=255) gCCATGTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCa < 1:2827779/70‑1 (MQ=255) gCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCaa > 1:1421609/1‑71 (MQ=255) gCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCaa > 1:3185728/1‑71 (MQ=255) gCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCaa < 1:3768999/71‑1 (MQ=255) cATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTAc > 1:3011332/1‑57 (MQ=255) cATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAAtt > 1:2864839/1‑71 (MQ=255) tCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCgg < 1:1807149/59‑1 (MQ=255) cTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGc > 1:144060/1‑71 (MQ=255) tGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGc > 1:3937316/1‑70 (MQ=255) tGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGc > 1:367958/1‑70 (MQ=255) tCCAGCGCCGCTCCCGTTATTGCAAAAAATTTCActct < 1:3021815/38‑1 (MQ=37) tCCAGCGCCGCTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCAtt < 1:1638478/71‑1 (MQ=255) aGCGCCGCTCCCGTTATGGCAAAAGATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCAtt < 1:2482914/68‑1 (MQ=255) aGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAAc < 1:2762660/71‑1 (MQ=255) aGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAAc < 1:1638736/71‑1 (MQ=255) gcagcTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCAt > 1:214328/4‑64 (MQ=255) ccgcTCCCGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCa < 1:1611384/71‑1 (MQ=255) ccgcTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTc > 1:999246/1‑70 (MQ=255) ccGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGc < 1:2019039/54‑1 (MQ=255) ccGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAAccc < 1:1252431/70‑1 (MQ=255) cGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCAt < 1:35853/71‑1 (MQ=255) cGTTATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCAt < 1:2837309/71‑1 (MQ=255) ttATGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTc < 1:2242457/44‑1 (MQ=255) ttATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCa < 1:1581975/45‑1 (MQ=255) ttATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAAcc < 1:2195180/66‑1 (MQ=255) tATGGCAAAACATTTCACTCTTGTGATCGCTAccc > 1:400838/1‑35 (MQ=255) aTGGCAAAACATTTCGCTCTTGTGATCGCTACCCGGAGTGTCa < 1:2544712/43‑1 (MQ=255) aTGGCAAAACATTTCACTCTTGTGATCGCTACCCGGa > 1:595039/1‑37 (MQ=255) aTGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAAtt > 1:752395/1‑46 (MQ=255) aTGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCt > 1:66178/1‑71 (MQ=255) tGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATa < 1:1681502/67‑1 (MQ=255) tGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATa < 1:3315478/67‑1 (MQ=255) gCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGc > 1:634883/1‑67 (MQ=255) cAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGgag < 1:3101420/71‑1 (MQ=255) aaaaCATTTCACTCTTGTGATCGCTACCGGGAGTGTCa < 1:438063/38‑1 (MQ=255) aaaaCATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCaaa > 1:688551/1‑57 (MQ=255) tttCGCTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGccc > 1:1294031/1‑71 (MQ=255) tttCACTCTTGTGATCGCTACCCGGAGTGTCAAttt < 1:2571188/36‑1 (MQ=255) cACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGCCCTg < 1:1154817/70‑1 (MQ=255) cACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGCCCTg < 1:3823411/70‑1 (MQ=255) | AATTACATGCCCCCAATGTCGGACGGGCCATCTGGTCCAGCGCCGCTCCCGTTATGGCAAAACATTTCACTCTTGTGATCGCTACCCGGAGTGTCAATTTGCCATTAACTTCAAACCCATAGCTGGAGAATGCCCTG > W3110S.gb/4208764‑4208900 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |