| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,225,730 | G→A | 31.1% | T117I (ACT→ATT) | arpA ← | regulator of acetyl CoA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,225,730 | 0 | G | A | 31.1% | 60.1 / 35.5 | 45 | T117I (ACT→ATT) | arpA | regulator of acetyl CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (13/18); new base A (4/10); total (17/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
TAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTA > W3110S.gb/4225662‑4225795 | tAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAgt < 1:628380/70‑1 (MQ=255)tAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAatg > 1:185512/1‑71 (MQ=255) aaGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAgtg > 1:2544938/1‑70 (MQ=255) aGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTc > 1:2615296/1‑71 (MQ=255) aTTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGc < 1:329055/70‑1 (MQ=255) aTTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCg < 1:3371002/71‑1 (MQ=255) ttCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGt > 1:290576/1‑71 (MQ=255) cTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGtt > 1:803324/1‑70 (MQ=255) gggAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGa < 1:3794895/69‑1 (MQ=255) gggAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGa < 1:363531/69‑1 (MQ=255) aaTTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAgg > 1:308714/1‑68 (MQ=255) aaTTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTa > 1:2757958/1‑70 (MQ=255) ttctttttctttTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTaa < 1:312161/69‑1 (MQ=255) ctttttcttttCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAAtt > 1:2038175/1‑71 (MQ=255) ctttttcttttCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAAtt > 1:151417/1‑71 (MQ=255) tttcttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAAtt < 1:858914/68‑1 (MQ=255) tttcttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc < 1:2979715/70‑1 (MQ=255) tttcttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc < 1:3536959/70‑1 (MQ=255) tttcttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc < 1:3677148/70‑1 (MQ=255) tttcttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTc < 1:152103/70‑1 (MQ=255) tttcttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAAtt < 1:3088856/68‑1 (MQ=255) tttcttttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTc < 1:3214364/70‑1 (MQ=255) tctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAgg < 1:1352406/70‑1 (MQ=255) tCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGGTGAGGTAAAATTTCCATTAGGTAt < 1:3778798/69‑1 (MQ=255) tGATTTTAGGTTTAATTCTTGTTTATCAAGAgtgt > 1:3951455/1‑35 (MQ=255) tGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTAtttt > 1:1599208/1‑70 (MQ=255) gATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCAt > 1:2820165/1‑59 (MQ=255) aTTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTc > 1:2693310/1‑70 (MQ=255) aTTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTc > 1:3880123/1‑70 (MQ=255) aTTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCa < 1:666772/71‑1 (MQ=255) ttAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCaaa < 1:2475838/70‑1 (MQ=255) ttAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCaaa < 1:3548051/70‑1 (MQ=255) ggTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGTTATTTTTCAAatat < 1:1157355/70‑1 (MQ=255) ttAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTggg > 1:2464148/1‑71 (MQ=255) ttCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTaaa < 1:908181/37‑1 (MQ=255) ttCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTaaa < 1:3544758/37‑1 (MQ=255) ttCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTaaa < 1:3215793/37‑1 (MQ=255) cTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAAt > 1:3621997/1‑37 (MQ=255) cTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAAt > 1:2952638/1‑37 (MQ=255) cTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCa > 1:2708261/1‑42 (MQ=255) cTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATg > 1:3058501/1‑71 (MQ=255) cTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATg > 1:1842285/1‑71 (MQ=255) tttATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGttt < 1:1929218/71‑1 (MQ=255) tCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGt < 1:3073521/71‑1 (MQ=255) cAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGc < 1:1691903/58‑1 (MQ=255) aaGAGTGTCTGAGCGTTGAGTTAAAATTTCCATTAGGTATTTTTCAAATATTgggtc < 1:3497958/57‑3 (MQ=255) aaGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTa < 1:3532523/70‑1 (MQ=255) aaGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTa < 1:2515142/70‑1 (MQ=255) aaGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTa < 1:1127229/70‑1 (MQ=255) | TAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTA > W3110S.gb/4225662‑4225795 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |