| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 338,000 | A→G | 14.8% | H151R (CAC→CGC) | yahG → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 338,000 | 0 | A | G | 14.8% | 115.5 / 13.1 | 50 | H151R (CAC→CGC) | yahG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (28/14); new base G (5/2); total (34/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01 | |||||||||||
CGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAA > W3110S.gb/337931‑338067 | cGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCAc < 1:2435688/71‑1 (MQ=255) gAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACg < 1:3425882/71‑1 (MQ=255) tcGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCGCGACTGCGTg > 1:4029691/1‑71 (MQ=255) tcGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCgag > 1:2249448/1‑69 (MQ=255) tcGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTg > 1:2981489/1‑71 (MQ=255) gacgaGGCGGATGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTgg > 1:2457319/1‑70 (MQ=255) gaGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATc < 1:3105380/71‑1 (MQ=255) tGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCGCGACTGCGTGGGATCGATGGCgg > 1:1091900/1‑71 (MQ=255) tGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCGCGACTGCGTGGGATCGATGGCgg > 1:2304884/1‑71 (MQ=255) tGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCgg > 1:1386896/1‑71 (MQ=255) tGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCgg > 1:1251545/1‑71 (MQ=255) cTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGgtgt > 1:4006146/1‑71 (MQ=255) gcggcTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTAc > 1:922973/1‑71 (MQ=255) gcTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTg > 1:3858097/1‑44 (MQ=255) cGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGcc < 1:2255914/70‑1 (MQ=255) cGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGcc < 1:2491428/70‑1 (MQ=255) gaTCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGt > 1:1855157/1‑71 (MQ=255) aTCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCTGGTGTTACCTCGGCCTCGATGtt > 1:2182866/1‑71 (MQ=255) aTCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGtt < 1:474930/71‑1 (MQ=255) tCACCTTCTCGCCGTGTCACGAGCGCGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGttt < 1:2957111/71‑1 (MQ=255) cACCTTCTCGCCGTGTCACGAGCGCGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTa > 1:2038257/1‑71 (MQ=255) cACCTTCTCGCCGTGTCACGAGCGCGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTa > 1:1071647/1‑71 (MQ=255) aCCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTa < 1:3687009/70‑1 (MQ=255) aCCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTa < 1:2621851/70‑1 (MQ=255) aCCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTAt < 1:3513098/71‑1 (MQ=255) ttCTCGCCGTGTGACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGtt < 1:163909/65‑1 (MQ=255) ttCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGtt < 1:113623/65‑1 (MQ=255) ttCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGca > 1:3618965/1‑71 (MQ=255) tctcGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGca > 1:831079/1‑70 (MQ=255) tctcGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGca > 1:895545/1‑70 (MQ=255) ctcGCCGTGTCACGAGGGCGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGcac < 1:3998695/70‑1 (MQ=255) ctcGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTa > 1:1985981/1‑65 (MQ=255) ctcGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTa > 1:2519086/1‑65 (MQ=255) ctcGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGcaca > 1:231404/1‑71 (MQ=255) ctcGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGcaca > 1:267411/1‑71 (MQ=255) ctcGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGcac > 1:68983/1‑70 (MQ=255) gCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCg > 1:2058446/1‑71 (MQ=255) gCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCg > 1:99820/1‑71 (MQ=255) aCGAGCTCGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaaaa > 1:954/1‑70 (MQ=255) aCGAGCACGACTGCGTGGGATCGATGGCGGGTGTTAc > 1:3308094/1‑37 (MQ=255) aCGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaaaa > 1:1357578/1‑70 (MQ=255) aCGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaaaa > 1:3517328/1‑70 (MQ=255) aCGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaaaa > 1:1534336/1‑70 (MQ=255) aCGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaaaa > 1:3082109/1‑70 (MQ=255) aCGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaaaa > 1:3075630/1‑70 (MQ=255) aCGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaaaa > 1:2414922/1‑70 (MQ=255) aCGAGCACGACTGCGTGGGATCGATGG‑GGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaac > 1:2976576/1‑70 (MQ=255) aGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaacaaa < 1:1687854/71‑1 (MQ=255) aGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaacaaa < 1:1962697/71‑1 (MQ=255) cACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAaaaacaaa < 1:3288125/69‑1 (MQ=255) aCGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTaaaa < 1:1199226/62‑1 (MQ=255) | CGAAAGATCTCGACGAGGCGGCTGAACTGGCGGCTTCCGGGGAGATCACCTTCTCGCCGTGTCACGAGCACGACTGCGTGGGATCGATGGCGGGTGTTACCTCGGCCTCGATGTTTATGCACATCGTAAAAAACAAA > W3110S.gb/337931‑338067 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |